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Zhamanbaeva G.T.,Al-Farabi Kazakh National University | Zhamanbaeva G.T.,Ben - Gurion University of the Negev | Murzakhmetova M.K.,Institute of Human and Animal Physiology | Murzakhmetova M.K.,Ben - Gurion University of the Negev | And 4 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2014

We studied the effects of ethanol extract from Hippophae rhamnoides L. leaves on the growth and differentiation of human acute myeloid leukemia cells (KG-1a, HL60, and U937). The extract of Hippophae rhamnoides L. leaves inhibited cell growth depending on the cell strain and extract dose. In a high concentration (100 μg/ml), the extract also exhibited a cytotoxic effect on HL60 cells. Hippophae rhamnoides L. leaves extract did not affect cell differentiation and did not modify the differentiating effect of calcitriol, active vitamin D metabolite. Inhibition of cell proliferation was paralleled by paradoxical accumulation of phase S cells (synthetic phase) with a reciprocal decrease in the count of G1 cells (presynthetic phase). The extract in a concentration of 100 μg/ml induced the appearance of cells with a subdiploid DNA content (sub-G1 phase cells), which indicated induction of apoptosis. The antiproliferative effect of Hippophae rhamnoides L. extract on acute myeloid leukemia cells was at least partially determined by activation of the S phase checkpoint, which probably led to deceleration of the cell cycle and apoptosis induction. © 2014, Springer Science+Business Media New York. Source


Seluyanov V.N.,Moscow State University | Kalinin E.M.,Moscow State University | Pack G.D.,Institute of Human and Animal Physiology | Mayevskaya V.I.,Institute of Human and Animal Physiology | Konrad A.N.,Latvian Academy of Sports Education
Human Physiology | Year: 2011

The goal of the study was to develop methods for estimating the anaerobic threshold from the rate of lung ventilation and heart rate variability during bicycle ergometer and treadmill tests with a stepwise increasing load. At the first stage, the method of estimation of the anaerobic threshold from lung ventilation data was developed. Forty-nine skilled ski racers participated in the experiment. They performed a treadmill ski-walking test with poles, with the slope gradually increasing from 0 to 25 degrees at a rate of one degree per minute. At the second stage, we developed a method for determining the anaerobic threshold from heart rate data. Eighty-six athletes of different sports specialties performed pedaling on a Monarch bicycle ergometer until exhaustion. The initial power was 25 W; the power increased by 25 W every 2 min. The pedaling rate remained constant (75 rpm). The lung ventilation, as well as oxygen consumption and carbon dioxide exhalation parameters, were measured using a COSMED K4 gas analyzer. Arterial blood was sampled from an earlobe or a finger; the blood lactate concentration was determined using an Akusport instrument. The RR intervals were recorded using a Polar s810i heart rate monitor. The results showed that the onset of ventilation anaerobic threshold (VAnT) determined by the graphical method coincided with the moment when blood lactate accumulated to 3. 8 ± 0. 1 mM in the treadmill test and 4. 1 ± 0. 6 mM in the bicycle ergometer test. The oxygen consumption at the VAnT level was found to be related to the variance of RR intervals (SD1). The following regression equation was derived: VO2AnT = 0. 35 + 0. 01SD1W + 0. 0016SD1HR + 0. 106SD1 (ms), l/min; (R = 0. 98, function estimation error, 0. 26 l/min, p < 0. 001), where W (W) is power, HR is heart rate (bpm), and SD1 is the variance of RR intervals (ms) at the moment of recording of the heart rate threshold. © 2011 Pleiades Publishing, Ltd. Source


Abdreshov S.N.,Institute of Human and Animal Physiology | Bulekbaeva L.E.,Institute of Human and Animal Physiology | Demchenko G.A.,Institute of Human and Animal Physiology
Bulletin of Experimental Biology and Medicine | Year: 2015

Dogs with experimental pancreatitis showed increased lymph flow, impaired rheological properties of the lymph and blood plasma, and increased plasma and blood levels of glucose, ALT, and AST. © 2015 Springer Science+Business Media New York. Source


Abdreshov S.N.,Institute of Human and Animal Physiology | Bulekbayeva L.E.,Institute of Human and Animal Physiology | Demshenko G.A.,Institute of Human and Animal Physiology
Bulletin of Experimental Biology and Medicine | Year: 2013

We studied contractile function of isolated mesenteric lymph nodes in rats with toxic hepatitis. We observed suppression of spontaneous and stimulated contractile activity of mesenteric lymph nodes and changes in biochemical composition of the lymph. We propose a method of correction of these dysfunctions with antioxidant α-tocopherol and Selen-Active. © 2013 Springer Science+Business Media New York. Source

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