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Jia X.,Tianjin Medical University | Liu J.,Tianjin Medical University | Gao Y.,Institute of Hepatobiliary Disease | Huang Y.,Tianjin Medical University | Du Z.,Tianjin Third Central Hospital
Archives of Medical Research | Year: 2014

Background and Aims: The diagnostic value of serum GPC3 in patients with hepatocellular carcinoma (HCC) remains controversial. Thus, we performed a systematic review and meta-analysis to assess the diagnostic accuracy of serum GPC3 for HCC. Methods: A systematic search was performed for the related studies. Sensitivity, specificity and other measures regarding the accuracy of serum GPC3 and alpha-fetoprotein (AFP) in the diagnosis of HCC were pooled using random-effects models. Summary receiver operating characteristic curve (sROC) analysis was used to summarize the overall test performance. Results: Nineteen studies were included in this meta-analysis. Pooled sensitivity, specificity and 95% confidence interval (CI) of serum GPC3 for the diagnosis of HCC were 55.2% (52.9-57.4%) and 84.2% (82.2-86.0%), respectively. When combining GPC3 with AFP, pooled sensitivity, specificity, and 95% CI were 75.7% (71.8-79.4%) and 83.3% (79.6-86.6%), respectively. The area under sROC (AUC) and 95% CI for AFP combined with GPC3 were 0.762 (0.649-0.875). For diagnosis of early HCC, pooled sensitivity and specificity of serum GPC3 were 55.1% (47.9-66.2%) and 97.0% (95.2-98.2%), respectively. The AUC of GPC3 for early HCC was 0.793 (0.668-0.917). Conclusions: This meta-analysis indicates that serum GPC3 has a comparable accuracy to AFP for the diagnosis of HCC, and there is an elevation in the sensitivity of diagnosis when GPC3 was combined with AFP. Diagnostic accuracy of serum GPC3 for early HCC is still unsatisfactory. © 2014 IMSS. Source


Shi J.,Tianjin Medical University | Sun Q.,The Third Central Hospital of Tianjin | Wang Y.,The Third Central Hospital of Tianjin | Jing X.,The Third Central Hospital of Tianjin | And 6 more authors.
Journal of Gastroenterology and Hepatology (Australia) | Year: 2014

Background and Aim: To compare the efficacy of microwave ablation (MWA) and surgical resection (RES) in the treatment of hepatocellular carcinoma (HCC) conforming to Milan criteria. Methods: Two hundred twenty-four patients met the inclusion criteria and were enrolled in the study. One hundred and seventeen patients received MWA, and 107 patients underwent RES. The primary endpoints were overall survival (OS) and disease-free survival (DFS). Results: The 1-, 3-, and 5-year OS rates were 94%, 70%, 52% for the MWA group and 94%, 72%, 60% for the RES group (P=0.513). The corresponding DFS rates for the two groups were 77%, 38%, 18% and 85%, 57%, 31% (P=0.005). In subgroup analyses of patients with solitary HCC ≤3cm, there were no significant differences in OS rates and DFS rates between the two groups (P=0.577 and P=0.140). For patients with solitary HCC 3 to 5cm, there was no significant differences in OS rates between the two groups (P=0.820), the DFS rates was significantly higher in the RES group than in the MWA group (P=0.014). Conclusions: MWA results in lower DFS rates than RES for HCC conforming to Milan criteria. However, the OS rates are comparable between the two therapies. For solitary HCC ≤3cm, MWA is as effective as RES. © 2014 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing Asia Pty Ltd. Source


Huang Y.,Tianjin Medical University | Huang Y.,Institute of Hepatobiliary Disease | Wang F.,Institute of Hepatobiliary Disease | Wang Y.,Institute of Hepatobiliary Disease | And 5 more authors.
Journal of Gastroenterology and Hepatology (Australia) | Year: 2014

Background and Aim: Recent studies have shown that imbalance between tumor-infiltrating interleukin (IL)-17+ T cells and regulatory T cells (Tregs) is an important regulator of progression in various cancers, but little is known regarding this imbalance in hepatocellular carcinoma (HCC). This study explored the role of imbalance between IL-17+ T cells and Tregs in the immunopathogenesis of HCC in patients with chronic hepatitis B (CHB) infection. Methods: Fifty-six of patient-matched tumors and peritumoral surgical specimens from 56 patient with HCC and 136 liver biopsies specimens from 46 patients with CHB, 37 with atypical hyperplasia (AH), and 53 with HCC were enrolled. The expressions of IL-17, FoxP3, CD4, and CD8 in liver tissue were measured by immunochemistry for the evaluation of liver-infiltrating lymphocytes. Results: The density of liver infiltrated FoxP3+ Tregs was increased in a stepwise manner from CHB to AH then HCC, while there was a decreasing trend for the density of IL-17+ T cells and CD8+ T cells. In surgical specimens of less differentiated HCC, the quantity of tumor-infiltrating FoxP3+ Tregs was significantly lower and IL-17+ T cells and CD8+ T cells were significantly higher. Additionally, peritumoral IL-17+ T cells were increased in poorly differentiated HCC. High intratumoral FoxP3+ Tregs with high intratumoral IL-17+ T cells showed a significantly lower overall survival (OS) and disease-free survival (DFS) compared with other groups (OS, P=0.033; DFS, P=0.004). High intratumoral FoxP3+ Tregs with high peritumoral IL-17+ T cells showed a significantly lower survival rate compared with other groups (OS, P<0.001 and DFS, P<0.001). Conclusion: Our findings suggest that intrahepatic IL-17+ T cells and FoxP3+ Tregs may cooperate to promote the progression of HCC. © 2013 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing Asia Pty Ltd. Source


Bai G.,Tianjin Third Central Hospital | Bai G.,Institute of Hepatobiliary Disease | Wu C.,Tianjin Third Central Hospital | Gao Y.,Institute of Hepatobiliary Disease | Shu G.,Tianjin Third Central Hospital
International Journal of Genomics | Year: 2015

This study has analyzed the gene expression patterns of an IPMN microarray dataset including normal pancreatic ductal tissue (NT), intraductal papillary mucinous adenoma (IPMA), intraductal papillary mucinous carcinoma (IPMC), and invasive ductal carcinoma (IDC) samples. And eight clusters of differentially expressed genes (DEGs) with similar expression pattern were detected by k-means clustering. Then a survey map of functional disorder in IPMN progression was established by functional enrichment analysis of these clusters. In addition, transcription factors (TFs) enrichment analysis was used to detect the key TFs in each cluster of DEGs, and three TFs (FLI1, ERG, and ESR1) were found to significantly regulate DEGs in cluster 1, and expression of these three TFs was validated by qRT-PCR. All these results indicated that these three TFs might play key roles in the early stages of IPMN progression. © 2015 Guiying Bai et al. Source


Xing Q.,Tianjin Medical University | Xing Q.,Institute of Hepatobiliary Disease | Luo Y.,Institute of Hepatobiliary Disease | Gao Y.,Institute of Hepatobiliary Disease | And 10 more authors.
Digestive and Liver Disease | Year: 2014

Background: Human induced pluripotent stem cells, which can be differentiated into hepatocyte-like cells, could provide a source for liver regeneration and bio-artificial liver devices. However, the functionality of hepatocyte-like cells is significantly lower than that of primary hepatocytes. Aims: To investigate whether serum from patients undergoing hepatectomy might promote differentiation from human induced pluripotent stem cells to hepatocyte-like cells. Methods: Serum from patients undergoing hepatectomy (acquired pre-hepatectomy and 3. hours, 1 day and 3 days post-hepatectomy) was used to replace foetal bovine serum when differentiating human induced pluripotent stem cells into hepatocyte-like cells. Properties of hepatocyte-like cells were assessed and compared with cells cultured in foetal bovine serum. Results: The differentiation efficiency and functionality of hepatocyte-like cells cultured in human serum 3. hours and 1 day post-hepatectomy were superior to those cultured in foetal bovine serum and human serum pre-hepatectomy. Human serum 3 days post-hepatectomy had an equal effect to that of human serum pre-hepatectomy. Some cytochrome P450 isozyme transcript levels of hepatocyte-like cells cultured in human serum were higher than those cultured in foetal bovine serum. Conclusion: Human serum, particularly that acquired relatively soon after hepatectomy, can enhance the differentiation efficiency and functionality of hepatocyte-like cells derived from human induced pluripotent stem cells. © 2014 Editrice Gastroenterologica Italiana S.r.l. Source

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