Time filter

Source Type

Liu M.,Hebei Medical University | Liu M.,Institute of Hebei Yiling Pharmaceutical Co. | Zhao S.,Institute of Hebei Yiling Pharmaceutical Co. | Jia J.,Institute of Hebei Yiling Pharmaceutical Co. | And 5 more authors.
Journal of Liquid Chromatography and Related Technologies | Year: 2011

Qualitative characterization and quantitative analysis of 15 bioactive constituents in Jiweiling freeze-dried powder (JWL) have been achieved by reversed-phase high performance liquid chromatography coupled with tandem mass spectrometry, including five flavonoids and ten ginsenosides. Chromatographic separation was performed on an Agilent ZORBAX Eclipse XDB-C18 HPLC column, with gradient elution of 0.1% formic acid aqueous solution and acetonitrile as mobile phase. The compounds were detected in the negative ion mode with multiple reaction monitoring (MRM) using a triple quadrupole mass spectrometer equipped with an electrospray ionization interface. By using of this method, 15 bioactive components were unequivocally identified on the basis of their characteristic fragmentation patterns. All calibration curves showed good linearity (r 2 > 0.998) within the test ranges. The LOD, LOQ, specificity, precision, and accuracy for the method were validated. The proposed method was successfully applied to analyze the quality of 12 batches of JWL. The results indicated that this analytical method was simple and suitable for the identification and quality assessment of JWL. Copyright © Taylor & Francis Group, LLC.


Liu M.,Hebei Medical University | Liu M.,Institute of Hebei Yiling Pharmaceutical Co. | Wang H.,Institute of Hebei Yiling Pharmaceutical Co. | Zhao S.,Institute of Hebei Yiling Pharmaceutical Co. | And 6 more authors.
Biomedical Chromatography | Year: 2011

A simple and rapid liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for analysis of ginsenoside Rb 1, Rb 2, Rc, Rd, Re, Rf, Rg 1, icariin and epimedin A, B, C in rat target tissues (spinal cord, brain, muscle and sciatic nerve) after intravenous administration of Jiweiling freeze-dried powder using genistein as an internal standard (IS). The tissue samples were treated by protein precipitation with methanol prior to HPLC and chromatographic separation was performed on a C 18 column utilizing a gradient elution program with acetonitrile and 0.1% formic acid aqueous. Electrospray ionization (ESI) source was employed and the 11 analytes and IS were detected by multiple reaction monitoring (MRM) scanning under the negative ionization mode. Higher sensitivity was achieved and the optimized mass transition ion-pairs (m/z) for quantitation were selected. The calibration curves were linear over the investigated concentration ranges with correlation coefficients higher than 0.995. The intra- and inter-day RSDs were all less than 10% with the relative error (RE) within ±9.3%. The mean extraction recoveries for all compounds were between 93.3 and 106%. The proposed method was successfully applied to investigate the target tissue distribution of the 11 compounds in rat after intravenous administration of Jiweiling freeze-dried powder. © 2011 John Wiley & Sons, Ltd.

Loading Institute of Hebei Yiling Pharmaceutical Co. collaborators
Loading Institute of Hebei Yiling Pharmaceutical Co. collaborators