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Chinju, South Korea

Azman K.F.,Universiti Sains Malaysia | Zakaria R.,Universiti Sains Malaysia | Abdaziz C.,Universiti Sains Malaysia | Othman Z.,Universiti Sains Malaysia | Al-Rahbi B.,Institute of Health science
Noise and Health | Year: 2015

Recent evidence has exhibited dietary influence on the manifestation of different types of behavior induced by stressor tasks. The present study examined the effects of Tualang honey supplement administered with the goal of preventing or attenuating the occurrence of stress-related behaviors in male rats subjected to noise stress. Forty-eight adult male rats were randomly divided into the following four groups: i) nonstressed with vehicle, ii) nonstressed with Tualang honey, iii) stressed with vehicle, and iv) stressed with honey. The supplement was given once daily via oral gavage at 0.2 g/kg body weight. Two types of behavioral tests were performed, namely, the novel object recognition test to evaluate working memory and the forced swimming test to evaluate depressive-like behavior. Data were analyzed by a two-way analysis of variance (ANOVA) using IBM SPSS 18.0. It was observed that the rats subjected to noise stress expressed higher levels of depressive-like behavior and lower memory functions compared to the unexposed control rats. In addition, our results indicated that the supplementation regimen successfully counteracted the effects of noise stress. The forced swimming test indicated that climbing and swimming times were significantly increased and immobility times significantly decreased in honey-supplemented rats, thereby demonstrating an antidepressant-like effect. Furthermore, cognitive function was shown to be intensely affected by noise stress, but the effects were counteracted by the honey supplement. These findings suggest that subchronic exposure to noise stress induces depressive-like behavior and reduces cognitive functions, and that these effects can be attenuated by Tualang honey supplementation. This warrants further studies to examine the role of Tulang honey in mediating such effects. Source


Jankowicz-Szymanska A.,Institute of Health science | Mikolajczyk E.,University School of Physical Education in Cracow
Pediatric Physical Therapy | Year: 2016

Purpose: To examine the relationship between obesity, genu valgum, and flat feet in children, and find practical implications for therapeutic interventions. Methods: A total of 1364 children aged 3-7 years took part in the research. Their body mass index was calculated and their weight status described. Participants' knee alignment was assessed by measuring the intermalleolar distance in the standing position with the knees in contact. The height of the longitudinal arch of each foot was measured using Clarke's angle. Results: The prevalence of overweight and obesity increased with age. Reduction of intermalleolar distance and increased longitudinal arch of the foot, characteristic of typical growth and development, were observed. Genu valgum was more common in children who were overweight. Significant correlations among body mass index, intermalleolar distance, and Clarke's angle (P <.05) were also discovered. Conclusion: Children who are overweight or demonstrate obesity are more likely to develop genu valgum and flat feet. © 2016 Wolters Kluwer Health, Inc. and Section on Pediatrics of the American Physical Therapy Association. Source


Joshi S.R.,Institute of Health science | Vasantha K.,National Institute of Immunohaematology
Asian Journal of Transfusion Science | Year: 2012

From transfusion point of view, a rare blood is the one which lacks a high-frequency antigen as well as the one who lacks multiple common antigens and such blood donations help in transfusion to those recipients having alloantibodies to corresponding antigens. In India, we have about four such kinds of phenotypes potential enough to pose problems in providing blood to the recipients having these phenotypes. Besides, there are other four kinds of rare bloods that pose seldom problems in blood supply, though some of these may cause problems in interpretation of results on assigning proper blood groups for a person. Source


Pan W.,CAS Shanghai Institutes for Biological Sciences | Zhu S.,Institute of Health science | Yuan M.,CAS Shanghai Institutes for Biological Sciences | Cui H.,CAS Shanghai Institutes for Biological Sciences | And 6 more authors.
Journal of Immunology | Year: 2010

Systemic lupus erythematosus is a complex autoimmune disease caused by genetic and epigenetic alterations. DNA methylation abnormalities play an important role in systemic lupus erythematosus disease processes. MicroRNAs (miRNAs) have been implicated as fine-tuning regulators controlling diverse biological processes at the level of posttranscriptional repression. Dysregulation of miRNAs has been described in various disease states, including human lupus. Whereas previous studies have shown miRNAs can regulate DNA methylation by targeting the DNA methylation machinery, the role of miRNAs in aberrant CD4+ T cell DNA hypomethylation of lupus is unclear. In this study, by using high-throughput microRNA profiling, we identified that two miRNAs (miR-21 and miR-148a) overexpressed in CD4+ T cells from both patients with lupus and lupus-prone MRL/lpr mice, which promote cell hypomethylation by repressing DNA methyltransferase 1 (DNMT1) expression. This in turn leads to the overexpression of autoimmune-associated methylation-sensitive genes, such as CD70 and LFA-1, via promoter demethylation. Further experiments revealed that miR-21 indirectly downregulated DNMT1 expression by targeting an important autoimmune gene, RASGRP1, which mediated the Ras-MAPK pathway upstream of DNMT1; miR-148a directly downregulated DNMT1 expression by targeting the protein coding region of its transcript. Additionally, inhibition of miR-21 and miR-148a expression in CD4+ T cells from patients with lupus could increase DNMT1 expression and attenuate DNA hypomethylation. Together, our data demonstrated a critical functional link between miRNAs and the aberrant DNA hypomethylation in lupus CD4+ T cells and could help to develop new therapeutic approaches. Copyright © 2010 by The American Association of Immunologists, Inc. Source


Noh H.,University of Georgia | Hong S.,University of Georgia | Dong Z.,University of Georgia | Pan Z.K.,University of Toledo | And 2 more authors.
Genes and Cancer | Year: 2011

Global mature microRNA (miRNA) expression is downregulated in cancers, and impaired miRNA processing enhances cancer cell proliferation. These findings indicate that the miRNA system generally serves as a negative regulator during cancer progression. In this study, we investigated the role of the miRNA system in cancer cell invasion by determining the effect of damaging miRNA processing on invasion-essential urokinase-type plasminogen activator (uPA) expression in breast cancer cells. Short hairpin RNAs specific for Drosha, DGCR8, and Dicer, key components of miRNA processing machinery, were introduced into 2 breast cancer cell lines with high uPA expression and 2 lines with poor uPA expression. Knockdown of Drosha, DGCR8, or Dicer led to even higher uPA expression in cells with high uPA expression, while it was unable to increase uPA level in cells with poor uPA expression, suggesting that the miRNA system most likely impacts uPA expression as a facilitator. In cells with high uPA expression, knockdown of Drosha, DGCR8, or Dicer substantially increased in vitro invasion, and depleting uPA abrogated enhanced invasion. These results thus link the augmented invasion conferred by impaired miRNA processing to upregulated uPA expression. uPA mRNA was a direct target of miR-193a/b and miR-181a, and a higher uPA level in cells with impaired miRNA processing resulted from less mature miR-193a/b and miR-181a processed from their respective primary miRNAs. Importantly, the levels of mature miR-193a, miR-193b, and miR-181a, but not their respective primary miRNAs, were lower in high uPA-expressing cells compared to cells with low uPA expression, and this apparently attributed to lower Drosha/DGCR8 expression in high uPA-expressing cells. This study suggests that less efficient miRNA processing can be a mechanism responsible for reduced levels of mature forms of tumor-suppressive miRNAs frequently detected in cancers. © The Author(s) 2011. Source

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