Institute of Health Care

Suwałki, Poland

Institute of Health Care

Suwałki, Poland
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Buzun L.,Medical University of Bialystok | Buzun L.,The Regional Specialist Hospital | Modzelewska B.,Medical University of Bialystok | Kostrzewska A.,Medical University of Bialystok | And 2 more authors.
Pflugers Archiv European Journal of Physiology | Year: 2017

The aim of the present study was to examine the contribution of intracellular and extracellular calcium sources in contraction caused by noradrenaline (NA) of the human internal thoracic artery (ITA) in vitro. Distal segments of ITA were obtained from 20 patients (aged 38–73, at the time of routine coronary artery surgical revascularization (CABG)). Contractile responses to 10−6 mol/L NA in the physiological salt solution and in Ca2+-free solution without and after incubation with 10−6 mol/L thapsigargin (TSG) were recorded under isometric conditions. Responses of ITA rings to 1 μM NA without incubation with TSG accounted (% of reaction to 80 mM KCl) 224.70 ± 14.06% in PSS solution, 141.30 ± 8.66% in Ca2+-free solution, and 80.03 ± 1.71% after PSS restoration and were statistically significantly different (p < 0.0001, one-way ANOVA). Responses of ITA rings to 1 μM NA with 1 μM TSG accounted (% of reaction to 80 mM KCl) 114.50 ± 2.79% in Ca2+-free solution and 36.70 ± 2.38% after PSS restoration. Responses in Ca2+-free solution and after PSS restoration without and with TSG were statistically significantly different (p = 0.0257 and p < 0.0001, respectively—t test). ITA contraction is caused by calcium derived not only from the SR and the extracellular matrix. The delivery of calcium to the space surrounding tissue does not immediately deliver calcium to the myofilaments. © 2017 Springer-Verlag Berlin Heidelberg


Kim T.-W.,Soonchunhyang University | Kim T.-W.,Institute of Health Care | Lee J.-B.,Soonchunhyang University
Applied Biochemistry and Biotechnology | Year: 2013

We assessed the effects of ingesting caffeine before passive heat loading (PHL) on serum leptin and sweating response, which are both physiological responses associated with energy expenditure. The subjects were nine male university students (age, 24.1 ± 3.5 years; height, 173.4 ± 7.6 cm; weight, 69.2 ± 5.7 kg; maximal oxygen consumption, 48.6 ± 4.7 ml × kg-1 × min-1). This study used a within-subject, random, crossover design. Tests were performed twice at the same time (2-5 p.m.) at a 1-week interval following 3 mg×kg-1 caffeine ingestion (Caff-I) or not (No-Caff). PHL included a half bath in hot water (42 ± 0.5 C for 30 min) in a thermoneutral climate chamber (25 ± 0.5 C, 60 ± 3 % relative humidity, <1 m/s air velocity). After PHL, blood levels of leptin and free fatty acids were significantly higher in the Caff-I compared to those in the No-Caff after PHL (P < 0.01). Waist circumference and whole-body sweat loss volume were significantly higher in the Caff-I compared to those in the No-Caff (P < 0.001). Mean active sweat gland density was significantly higher in the Caff-I compared to those in the No-Caff at 10 min during PHL (P < 0.001). The results suggest that ingesting caffeine before PHL is more energy efficient than that of a single PHL. © 2013 Springer Science+Business Media New York.


PubMed | Institute of Health Care, University of Lodz, Medical University of Gdańsk, John Paul II Hospital and 2 more.
Type: Journal Article | Journal: Experimental physiology | Year: 2016

What is the central question of this study? The main aim of the present study was to determine the effect of prolonged moderate-intensity endurance training on the endothelial glycocalyx layer integrity in relationship to the training-induced changes in oxidative stress and antioxidant defence in humans. What is the main finding and its importance? We have shown, for the first time, a protective effect of prolonged moderate-intensity endurance training on endothelial glycocalyx layer integrity, as judged by significantly lower basal and end-exercise serum concentrations of glycocalyx damage markers, i.e. syndecan-1 and heparan sulfate, accompanied by attenuation of oxidative stress and enhancement of antioxidant defence after training in previously untrained healthy young men. In this study, we evaluated the effect of 20weeks of moderate-intensity endurance training (ET) on the endothelial glycocalyx layer integrity in relationship to the training-induced changes in antioxidant defence. Eleven healthy young, untrained men performed an incremental cycling exercise bout until exhaustion before and after 20weeks of ET. Endurance training consisted of 40min sessions, mainly of moderate intensity (50% of maximal oxygen uptake), performed four times per week. Venous blood samples were taken at rest and at the end of the maximal exercise test. Muscle biopsies from vastus lateralis were taken before and after the training. Endurance training resulted in a significant increase in physical capacity (P<0.05) as reflected by an increase in power output reached at the lactate threshold and at maximal oxygen uptake. Training led to a decrease (P<0.05) in basal and end-exercise concentrations of blood markers of glycocalyx damage (syndecan-1 and heparan sulfate). The lowering of glycocalyx shedding after the ET was accompanied by an attenuation of oxidative stress, as evidenced by a decrease in the basal plasma concentration of isoprostanes, and by an increase in antioxidant defence, reflected by an enhancement in superoxide dismutase 2 protein content in vastus lateralis (P<0.05). In contrast, training did not induce a significant increase in basal nitrite/nitrate plasma concentration (P>0.05). Moderate-intensity ET exerts a pronounced protective effect on endothelial glycocalyx integrity at rest and during exercise, probably through an improvement of antioxidant defence that may represent the vasoprotective mechanisms highly responsive to moderate-intensity endurance training.


Kleszczewski T.,Medical University of Bialystok | Kleszczewska E.,Institute of Health Care | Buzun L.,Medical University of Bialystok | Modzelewska B.,Medical University of Bialystok
Journal of Trace Elements in Medicine and Biology | Year: 2016

Background: The aim of this study was the simultaneous determination of levels of cadmium and l-ascorbic Acid (AA) in human saphenous vein (SV) used in coronary artery bypass grafting (CABG) and check whether there is a relationship between these levels. Methods: Human SV were collected from 40 patients (20 men and 20 women; age, 40-75 years) at the time of routine coronary artery surgical revascularization. The concentration of cadmium in the tissue was determined according to the GF AAS-atomic absorption method. The concentration of AA was assayed in supernatant by FIA method with spectrophotometric detection. Results: AA concentration (mean ± SD); men: 98,7 ± 13,18 μg/g tissue, women: 96,06 ± 11,98 μg/g tissue. Cadmium concentration(mean ± SD); men: 309 ± 103,71 ng/g tissue, women: 348,5 ± 255,71 ng/g tissue. Correlations among concentrations of AA and cadmium were insignificant negative in the group of men (Pearson r = -0,1504, p = 0,5269) and in the group women (Pearson r = -0339, p = 0144). Conclusions: Negative correlations among concentrations of AA and cadmium in human SV obtained in our study may indicate a protective effect of this vitamin in relation to toxic cadmium. © 2016 Elsevier GmbH.


Mogielnicki A.,Medical University of Bialystok | Mogielnicki A.,University of Warmia and Mazury | Kramkowski K.,Medical University of Bialystok | Hermanowicz J.M.,Medical University of Bialystok | And 3 more authors.
JRAAS - Journal of the Renin-Angiotensin-Aldosterone System | Year: 2014

Introduction: ACE2 alternatively converts angiotensin (Ang) II into Ang-(1-7) and Ang I into Ang-(1-9). There is little information in the literature with respect to Ang-(1-9) properties. A number of studies show a link between peptides of the renin-angiotensin system and thrombosis. Materials and methods: We have investigated the influence of Ang-(1-9) on stasis-induced venous thrombosis in the rat. The contribution of coagulation and fibrinolytic systems, angiotensin receptor type 1 (AT1) and MAS receptor in the mode of Ang-(1-9) action was also determined. Results: Ang-(1-9) enhanced thrombosis development, decreased plasma concentration of tissue plasminogen activator and increased the level of its inhibitor (PAI-1). The action of Ang-(1-9) was reversed by selective antagonist of AT1 receptor, but not Ang-(1-7) antagonist. Ang-(1-9) did not bind to the AT1 receptor. Conclusions: Ang-(1-9) enhances venous thrombosis in the rat because of the impairment of fibrinolysis. The prothrombotic effect of Ang-(1-9) is mediated by Ang II acting via the AT1 receptor. © The Author(s) 2014.


PubMed | Medical University of Bialystok and Institute of Health Care
Type: | Journal: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) | Year: 2016

The aim of this study was the simultaneous determination of levels of cadmium and l-ascorbic Acid (AA) in human saphenous vein (SV) used in coronary artery bypass grafting (CABG) and check whether there is a relationship between these levels.Human SV were collected from 40 patients (20 men and 20 women; age, 40-75 years) at the time of routine coronary artery surgical revascularization. The concentration of cadmium in the tissue was determined according to the GF AAS-atomic absorption method. The concentration of AA was assayed in supernatant by FIA method with spectrophotometric detection.AA concentration (meanSD); men: 98,713,18g/g tissue, women: 96,0611,98g/g tissue. Cadmium concentration(meanSD); men: 309103,71ng/g tissue, women: 348,5255,71ng/g tissue. Correlations among concentrations of AA and cadmium were insignificant negative in the group of men (Pearson r=-0,1504, p=0,5269) and in the group women (Pearson r=-0339, p=0144).Negative correlations among concentrations of AA and cadmium in human SV obtained in our study may indicate a protective effect of this vitamin in relation to toxic cadmium.


PubMed | Specialist Dental Practice, Medical University of Bialystok and Institute of Health Care
Type: Journal Article | Journal: Advances in clinical and experimental medicine : official organ Wroclaw Medical University | Year: 2016

Matrix metalloproteinases (MMPs) belong to a family of structurally related zinc-dependent proteolytic enzymes that are known to play a key role in the catabolic turnover of extracellular matrix (ECM) components. Research studies to date have indicated that MMPs regulate the activity of several non-ECM bioactive substrates, including growth factors, cytokines, chemokines and cell receptors, which determine the tissue microenvironment. Disruption of the balance between the concentration of active matalloproteinases and their inhibitors (TIMPs) may lead to pathological changes associated with uncontrolled ECM turnover, tissue remodeling, inflammatory response, cell growth and migration. This brief review presents some information on MMPs role in inflammatory, metabolic and cancer abnormalities related to the salivary glands, as well as MMP-related aspects that lead to the formation of human dentinal caries lesions. In oral diseases, the most relevant biological fluid commonly used for diagnosing periodontal diseases is saliva. In diseased patients with significantly higher levels of MMPs in their saliva than healthy people, most extracellular matrix components undergo digestion to lower molecular weight forms. Conventional treatment successfully reduces the levels of MMPs inhibits the progressive breakdown of gingival and periodontal ligament collagens. Beside inflammatory abnormalities like Sjgrens syndrome (SS), a large group of disorders is comprised of cancers, most of them involving the parotid gland.


PubMed | Medical University of Bialystok and Institute of Health Care
Type: | Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | Year: 2016

One strong risk factor of coronary artery disease (CAD), which affects the levels of vitamin C in the blood is cigarette smoking. The supplementary effects of coronary artery bypass grafting (CABG) is smoking cessation by patients. Therefore, the aim of this study was to examine the level of vitamin C in the blood plasma one day before and one month after CABG. Human blood were collected from 20 patients (men); 1day before and 1 month after CABG. All patients were smoker and after CABG they declared their not smoking. The concentration of vitamin C in the blood plasma was assayed by FIA method with spectrophotometric detection. The mean value of the vitamin C concentration 1day before CABG was 12.362.84mol/L (meanSD), 1 month after CABG 40.0710.95mol/L (meanSD). The average increase in the concentration of vitamin C was 3.270.73 times (meanSD) and showed significant positive correlations (Pearson r=0.657, p=0.002). It should be consider incorporating the recommendations of preoperative smoking cessation for at least one month prior to CABG and/or additional supplementation. In addition it would be relevant to monitor the level of vitamin C in the patients blood in the preoperative period.


PubMed | Medical University of Bialystok, Polish Academy of Sciences and Institute of Health Care
Type: | Journal: Reproductive toxicology (Elmsford, N.Y.) | Year: 2016

Copper may influence the in vivo and in vitro uterine activity. Recent evidence shows that cupric ions can easily form complexes with oligopeptides like oxytocin (OXT). The high complex stability in vitro suggests a possibility of complex formation in vivo.In vitro isometric contractions were recorded in uterine tissues from pregnant women undergoing caesarean sections and the effect of OXT and the Cu-OXT complex on isolated human pregnant myometrium was investigated.In the concentration range from 10(-14) to 10(-6)M of OXT alone, pre-formed Cu-OXT complex, and OXT following sample preincubation with Cu(II) salt, nosignificant differences were observed for the following parameters of pregnant uterine smooth muscle contraction: the area under the curve, frequency and amplitude of contraction.The binding of Cu(2+) ions does not abolish the ability of OXT to interact with oxytocin receptors and stimulate myometrial contraction in vitro.


PubMed | Medical University of Bialystok and Institute of Health Care
Type: Journal Article | Journal: Pharmacological reports : PR | Year: 2015

Aliskiren is the first orally active inhibitor of renin to be approved for clinical use as an antihypertensive agent. A number of studies show a link between aliskiren and intravascular thrombosis.The goal of the present study was to investigate the impact of aliskiren on arterial thrombosis in normotensive and renovascular hypertensive rats. The contribution of each coagulation and fibrinolytic parameters in the mode of aliskiren action was determined. Six weeks after clipping of the left renal artery rats developed hypertension which was confirmed by the tail cuff method. Animals were treated with aliskiren (10, 30 and 100mg/kg/day) per os for 10 days. Arterial thrombosis was induced by electrical stimulation of the common carotid artery.It was found that aliskiren in a dose-dependent manner decreased weight of the arterial thrombus in normotensive and hypertensive rats. It has been shown that this result was not associated with the effects on blood pressure, TF, PT, APTT, fibrinogen and hematological parameters. It was found that aliskiren caused increase of t-PA activity and decrease of its inhibitor activity.The presented results indicate that aliskiren inhibits hemostasis in the arterial thrombosis in rats. The antithrombotic effect is related with improvement of the fibrinolytic balance, and also depends on antiplatelet action.

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