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Hadong, South Korea

Song H.J.,Gyeongsang National University | Kim Y.D.,Institute of Hadong Green Tea | Jeong M.J.,Korea National Arboretum | Ahn M.S.,Korea Research Institute of Bioscience and Biotechnology | And 3 more authors.
Plant Biotechnology Reports | Year: 2015

A simple and rapid ninhydrin-based colorimetric method was efficiently developed, and metabolites profiling of tea tree (Camellia sinensis L.) leaf extracts was carried out by Fourier transform near-infrared (FT-IR) spectroscopy. The tea extracts of Hadong region exhibited a wide range of variations in their theanine contents. In general, theanine and other amino acids were higher in classified theanine-rich lines than that of theanine-poor lines. Among selected theanine-rich lines, the theanine levels reached a maximum of 15.3 mg/g for line TR92, a value 51-fold more than that found in theanine-poor TP23 and TP156 lines. Therefore, ninhydrin-based colorimetric method can be efficiently adopted for the selection of theanine-rich plants thereby enabling selection of plants dependent on their chemical constituents. FT-IR spectroscopy analysis was conducted in 15 tea tree lines as theanine-rich 8 lines and -poor 7 lines. The spectral data were analyzed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). PCA and PLS-DA could successfully discriminate theanine-rich and -poor lines. The quantitative prediction modeling of total amino acids and theanine from tea tree leaf lines was established using partial least square regression algorithm from FT-IR spectra. The regression coefficients (R2) between predicted values and estimated values of total amino acids and theanine were 0.976 and 0.992, respectively. These results showed that quantitative predictions of total amino acids and theanine were possible from FT-IR spectra of tea tree leaf lines with higher accuracy. These results suggest that the prediction system established in this study could be applied as a rapid selection of theanine-rich lines of tea tree. © 2015, Korean Society for Plant Biotechnology and Springer Japan. Source

Jeong H.R.,Gyeongsang National University | Choi G.N.,Gyeongsang National University | Kim J.H.,Gyeongsang National University | Kwak J.H.,Gyeongsang National University | And 4 more authors.
Korean Journal of Food Science and Technology | Year: 2010

The nutritional components, antioxidant, and neuroprotective effects of water and a 50% methanol extract from litchi fruit pericarp were investigated. The most abundant mineral, amino acid, and fatty acid were K, proline, and palmitic acid, respectively. In addition, the total water phenolics and 50% methanol extracts were 8.02 and 12.28 mg/g, respectively. The DPPH, ABTS radical scavenging activities and ferric reducing antioxidant power of the water and 50% methanol extracts showed dose-dependent antioxidant activity. In a cell viability assay using MTT, almost all extracts showed a protective effect against H2O2-induced neurotoxicity, and lactate dehydrogenase leakage was also inhibited by the pericarp extracts. In particular, the 50% methanol extract showed a higher cell membrane protective effect than the water extract at the highest concentration. Consequently, these data suggest that litchi fruit pericarp can be utilized as an effective and safe functional food substances for natural antioxidants and may reduce the risk of neurodegenerative disorders. © The Korean Society of Food Science and Technology. Source

Kim Y.D.,Institute of Hadong Green Tea | Kang S.M.,Korea forest Research Institute | Min J.Y.,Gyeongsang National University | Choi W.K.,Gyeongsang National University | And 3 more authors.
Journal of Natural Products | Year: 2010

The production of tropane alkaloids during differentiation and de-differentiation of Scopolia parviflora calli was studied. Tropane alkaloid production drastically decreased during calli de-differentiation. Scopolamine (1) production decreased after 10 days of culture, whereas that of hyoscyamine (2) decreased during de-differentiation of root to calli. The production of 1 was enhanced in calli undergoing differentiation to shoot after 60 days of culture, reaching a maximum by 80 days. However, production of hyoscyamine in regenerated plants was lower. The expression level of hyoscyamine 6/3-hydroxylase (H6H), a key biosynthetic enzyme for tropane alkaloids, was significantly increased in 4-week-old calli. This study suggests that the biosynthesis of tropane alkaloids is regulated inversely in de-differentiating Scopolia parviflora calli. © 2010 American Chemical Society and American Society of Pharmacognosy. Source

Kim Y.D.,Institute of Hadong Green Tea | Yun J.G.,Gyeongnam National University of Science and Technology | Seo Y.R.,Gyeongsang National University | Karigar C.S.,Tumkur University | Choi M.S.,Gyeongsang National University
Korean Journal of Horticultural Science and Technology | Year: 2015

Effects of mineral salts (N, P, K, Ca2+, Mg2+, and Fe3+) on the shoot growth and metabolite production of tea tree were s tudied u sing in v itro culture techniques. Among mineral s alts, H2PO4 - was the most important for enhanced growth rate of tea tree, while Mg2+ and Ca2+ did not a ffect plant growth. Remov al o f NH4 + and NO3 from the culture medium enhanced shoot multiplication compared to other treatments. Metabolite production was variable depending on mineral types and concentration. Removal of Ca2+ decreased the production of caffeine; however, other treatments did not influence its production. Ca2+, NH4 + and Fe3+ were important factors for catechin production in tea tree. These results can be used as the basis for development of technical soil controls suitable for tea tree cultivation in the future. © 2015 Korean Society for Horticultural Science. Source

Heo C.-M.,Gyeongsang National University | Heo C.-M.,Kyushu University | Kim Y.-D.,Institute of Hadong Green Tea | Kim Y.-D.,Kyushu University | And 22 more authors.
Journal of the Faculty of Agriculture, Kyushu University | Year: 2012

An efficient and rapid colorimetric method was established for the selection of tea tree lines rich in polyphenol from 160 tea tree lines. The efficient selection method was made possible by the adoption of the Folin-Ciocalteu (FC) colorimetric method for qualitative detection of the polyphenols. The polyphenol composition varied widely among the randomly sampled 160 tea tree lines. The tea tree lines were classified into three groups on the basis of color intensity obtained after treatment of tea extracts with FC reagent. The polyphenol content of the three groups was quantified and validated by UV spectrophotometric analysis. Among various tea lines, H-23 tea line exhibited the highest amounts of polyphenol that was 2.89-fold higher than those found in low polyphenol producing tea tree (H-9). Hence FC colorimetric method can become a reliable selection method for easy and rapid selection of tea population rich in polyphenol in a tea tree breeding programme. Source

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