Institute of Hadong Green Tea

Hadong, South Korea

Institute of Hadong Green Tea

Hadong, South Korea
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Jo Y.-H.,Kyungnam University | Yuk H.-G.,National University of Singapore | Lee J.-H.,Andong National University | Kim J.-C.,Institute of Hadong Green Tea | And 2 more authors.
Food Science and Biotechnology | Year: 2012

The present study evaluates antioxidant, tyrosinase inhibitory, and acetylcholinesterase (AChE) inhibitory activities of seed and its pericarp of tea plant (Camellia sinensis L.). Water and methanol extracts of tea seed and pericarp were prepared in a shaking incubator overnight at room temperature. The highest total phenolic contents, DPPH and ABTS radical scavenging activities, reducing power, β-carotene bleaching inhibition activity, highest tyrosinase inhibitory activity, and AChE inhibitiory activity were found in the methanol extracts of pericarp. Caffeine, gallic acid, several phenolic compounds, hydroxymethyl furfural, and fatty acid derived compounds were detected in the extract of tea seed and pericarp. The results indicate that seed and pericarp could be utilized as the potential resources for antioxidant ingredients in food industry. In addition, these compounds may protect Alzheimer's disease as they had inhibitory activity of acetylcholine esterase.

Kim Y.D.,Institute of Hadong Green Tea | Yun J.G.,Gyeongnam National University of Science and Technology | Seo Y.R.,Gyeongsang National University | Karigar C.S.,Tumkur University | Choi M.S.,Gyeongsang National University
Korean Journal of Horticultural Science and Technology | Year: 2015

Effects of mineral salts (N, P, K, Ca2+, Mg2+, and Fe3+) on the shoot growth and metabolite production of tea tree were s tudied u sing in v itro culture techniques. Among mineral s alts, H2PO4 - was the most important for enhanced growth rate of tea tree, while Mg2+ and Ca2+ did not a ffect plant growth. Remov al o f NH4 + and NO3 from the culture medium enhanced shoot multiplication compared to other treatments. Metabolite production was variable depending on mineral types and concentration. Removal of Ca2+ decreased the production of caffeine; however, other treatments did not influence its production. Ca2+, NH4 + and Fe3+ were important factors for catechin production in tea tree. These results can be used as the basis for development of technical soil controls suitable for tea tree cultivation in the future. © 2015 Korean Society for Horticultural Science.

Heo C.-M.,Gyeongsang National University | Heo C.-M.,Kyushu University | Kim Y.-D.,Institute of Hadong Green Tea | Kim Y.-D.,Kyushu University | And 22 more authors.
Journal of the Faculty of Agriculture, Kyushu University | Year: 2012

An efficient and rapid colorimetric method was established for the selection of tea tree lines rich in polyphenol from 160 tea tree lines. The efficient selection method was made possible by the adoption of the Folin-Ciocalteu (FC) colorimetric method for qualitative detection of the polyphenols. The polyphenol composition varied widely among the randomly sampled 160 tea tree lines. The tea tree lines were classified into three groups on the basis of color intensity obtained after treatment of tea extracts with FC reagent. The polyphenol content of the three groups was quantified and validated by UV spectrophotometric analysis. Among various tea lines, H-23 tea line exhibited the highest amounts of polyphenol that was 2.89-fold higher than those found in low polyphenol producing tea tree (H-9). Hence FC colorimetric method can become a reliable selection method for easy and rapid selection of tea population rich in polyphenol in a tea tree breeding programme.

Song H.J.,Gyeongsang National University | Kim Y.D.,Institute of Hadong Green Tea | Jeong M.J.,Korea National Arboretum | Ahn M.S.,Korea Research Institute of Bioscience and Biotechnology | And 3 more authors.
Plant Biotechnology Reports | Year: 2015

A simple and rapid ninhydrin-based colorimetric method was efficiently developed, and metabolites profiling of tea tree (Camellia sinensis L.) leaf extracts was carried out by Fourier transform near-infrared (FT-IR) spectroscopy. The tea extracts of Hadong region exhibited a wide range of variations in their theanine contents. In general, theanine and other amino acids were higher in classified theanine-rich lines than that of theanine-poor lines. Among selected theanine-rich lines, the theanine levels reached a maximum of 15.3 mg/g for line TR92, a value 51-fold more than that found in theanine-poor TP23 and TP156 lines. Therefore, ninhydrin-based colorimetric method can be efficiently adopted for the selection of theanine-rich plants thereby enabling selection of plants dependent on their chemical constituents. FT-IR spectroscopy analysis was conducted in 15 tea tree lines as theanine-rich 8 lines and -poor 7 lines. The spectral data were analyzed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). PCA and PLS-DA could successfully discriminate theanine-rich and -poor lines. The quantitative prediction modeling of total amino acids and theanine from tea tree leaf lines was established using partial least square regression algorithm from FT-IR spectra. The regression coefficients (R2) between predicted values and estimated values of total amino acids and theanine were 0.976 and 0.992, respectively. These results showed that quantitative predictions of total amino acids and theanine were possible from FT-IR spectra of tea tree leaf lines with higher accuracy. These results suggest that the prediction system established in this study could be applied as a rapid selection of theanine-rich lines of tea tree. © 2015, Korean Society for Plant Biotechnology and Springer Japan.

Jeong H.R.,Gyeongsang National University | Choi G.N.,Gyeongsang National University | Kim J.H.,Gyeongsang National University | Kwak J.H.,Gyeongsang National University | And 4 more authors.
Korean Journal of Food Science and Technology | Year: 2010

The nutritional components, antioxidant, and neuroprotective effects of water and a 50% methanol extract from litchi fruit pericarp were investigated. The most abundant mineral, amino acid, and fatty acid were K, proline, and palmitic acid, respectively. In addition, the total water phenolics and 50% methanol extracts were 8.02 and 12.28 mg/g, respectively. The DPPH, ABTS radical scavenging activities and ferric reducing antioxidant power of the water and 50% methanol extracts showed dose-dependent antioxidant activity. In a cell viability assay using MTT, almost all extracts showed a protective effect against H2O2-induced neurotoxicity, and lactate dehydrogenase leakage was also inhibited by the pericarp extracts. In particular, the 50% methanol extract showed a higher cell membrane protective effect than the water extract at the highest concentration. Consequently, these data suggest that litchi fruit pericarp can be utilized as an effective and safe functional food substances for natural antioxidants and may reduce the risk of neurodegenerative disorders. © The Korean Society of Food Science and Technology.

Kim Y.D.,Institute of Hadong Green Tea | Jeong M.J.,Gyeongsang National University | Song H.J.,Gyeongsang National University | Kim J.C.,Institute of Hadong Green Tea | Choi M.S.,Gyeongsang National University
Forest Science and Technology | Year: 2012

Catechins-rich and -poor tea tree lines were selected through a colorimetric selection method from 160 tea tree lines used in previous studies. The selected tea tree lines, catechins-rich lines (HR-52, HR-29, HR-82, HR-123, and HR- 55) and catechins-poor lines (HP-19, HP-108, HP-138, HP-150, and HP-18), were analyzed through morphological (leaf width, leaf length, leaf area, and shoot length) and genetic analysis. The cluster pattern of morphological characters showed a positive correlation between the catechins contents and the leaf morphology. In RAPD analysis, the amplified DNA bands did not appear with 10 primers, however, the various sizes of DNA bands were detected with 22 primers. The special DNA bands between the rich and poor lines were not found. These results suggest that the morphological and genetic character may be somewhat related to secondary metabolites of tea trees. © 2012 Korean Forest Society.

Kim Y.D.,Institute of Hadong Green Tea | Min J.Y.,Gyeongsang National University | Jeong M.J.,Gyeongsang National University | Song H.J.,Gyeongsang National University | And 4 more authors.
Journal of Wood Science | Year: 2010

A rapid and efficient colorimetric method based on the use of Fast Blue B-salt (FBB) was established to select catechin-rich tea trees (Camellia sinensis L.). The catechin levels measured by the colorimetric method under optimized reaction conditions correlated closely with estimations by high-performance liquid chromatography (HPLC) analysis. The FBB colorimetric method was successfully used to classify 160 tea trees on the basis of their catechin contents into rich and poor lines. HPLC analysis of the FBB-selected tea tree extracts showed them to contain (-)-epigallocatechin 186 mg/g in tea tree line HR-29, (-)-epicatechin 43.7 mg/g in HR-82, (-)-epigallocatechin gallate 4.32 mg/g in HR-29, and (-)-epicatechin gallate 0.22 mg/g in HR-52. Classification of tea trees from the Hadong region into catechin-rich and -poor trees was independent of the growing season. Thus the FBB colorimetric method could find application as a reliable tool in screening and selection of tea trees on the basis of their catechin content. © 2010 The Japan Wood Research Society.

Kim Y.D.,Institute of Hadong Green Tea | Kang S.M.,Korea forest Research Institute | Min J.Y.,Gyeongsang National University | Choi W.K.,Gyeongsang National University | And 3 more authors.
Journal of Natural Products | Year: 2010

The production of tropane alkaloids during differentiation and de-differentiation of Scopolia parviflora calli was studied. Tropane alkaloid production drastically decreased during calli de-differentiation. Scopolamine (1) production decreased after 10 days of culture, whereas that of hyoscyamine (2) decreased during de-differentiation of root to calli. The production of 1 was enhanced in calli undergoing differentiation to shoot after 60 days of culture, reaching a maximum by 80 days. However, production of hyoscyamine in regenerated plants was lower. The expression level of hyoscyamine 6/3-hydroxylase (H6H), a key biosynthetic enzyme for tropane alkaloids, was significantly increased in 4-week-old calli. This study suggests that the biosynthesis of tropane alkaloids is regulated inversely in de-differentiating Scopolia parviflora calli. © 2010 American Chemical Society and American Society of Pharmacognosy.

Jeong J.-S.,Wild Flower Institute | Kim Y.-J.,Wild Flower Institute | Choi B.-R.,Wild Flower Institute | Park N.-J.,Gurye gun Agricultural Center | And 6 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2013

To promote the utilization of wild edible plants, this study examined blanching, drying, and fermentation as methods for enhancing the functionality of Hemerocallis coreana Nakai. Specimens fermented for 24 hours at a fermentation temperature of 50°C, with a relative humidity of 65%, contained the highest amount of organic acid (18,109.82 mg/100 g). For the blanched; specimens, total organic acid content decreased about 30% compared with the freeze-dried specimens. The main organic acid of Hemerocallis coreana Nakai was confirmed as succinic acid. After fermentation, free sugars decreased; in particular, specimens fermented at a relative humidity of 80% showed a 32~75% reduction in free sugar compared with the freeze-dried specimens. In terms of amino acid content, Hemerocallis coreana Nakai was mainly composed of valine, isoleucine, and phenylalanine. In fermented specimens the total amino acid content was highest in a moderately fermented (17 hr) specimen, (1,010.71 mg/100 g fresh wt.), but decreased in the maximally fermented (24 hr) specimen. The longer the fermentation, the higher the decrease in non-essential amino acids content, while the content of more essential amino acids consistently increased. In conclusion, since seasoned Hemerocallis coreana Nakai contains a considerable amount of glutamine and asparagine, it has a fresh sour and sweet taste; thus, it will likely be a highly preferred wild edible plant. Also, with an increase of essential amino acids after fermentation, Hemerocallis coreana Nakai is excellent in terms of nutrition. Thus, it may be possible to utilize fermented Hemerocallis coreana Nakai in the development of diverse products.

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