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Lednice, Czech Republic

Kudelkova M.,Mendeleum Institute of Genetics | Ondrusikova E.,Mendeleum Institute of Genetics
Acta Horticulturae

Meristem isolation and chemotherapy were used for Garlic common latent virus (GCLV) elimination in garlic (Allium sativum L.). Five of GCLV infected garlic genotypes were chosen for the experiment. Ribavirin at concentrations of 25 or 50 mg L-1 was used for chemotherapy. The effects of chemotherapy and meristem isolation were assessed. The presence of the virus was indexed by ELISA tests. The best result for GCLV elimination was chemotherapy using 25 mg L-1 of ribavirin. In this treatment, GCLV was eliminated in 100% of the plants of 'N9A', 'Anton', and 'Tristan' genotypes. In 'Tristan' and 'Anton' 100% of plantlets were indexed virus negative after using 50 mg L-1 of ribavirin. No virus free plant was achieved in the case of genotype 'Mako' using meristem isolation. The highest number of plants negatively indexed for GCLV was achieved using 25 mg L-1 ribavirin. Source

Kudelkova M.,Mendeleum Institute of Genetics | Eichmeier A.,Mendeleum Institute of Genetics | Baranek M.,Mendeleum Institute of Genetics | Cechova J.,Mendeleum Institute of Genetics
Acta Horticulturae

The method was developed for the detection and resolution of the Garlic common latent virus that causes a serious disease of economically important plants of Allium species, in the Potyvirus presence particularly. GCLV was detected in garlic from Asia, Europe, South America and also from North America. GCLV together with SLV were the most widespread in the collection, as they occurred in 82 and 83% of the accessions in the Czech Republic as was described earlier. GCLV belong to ssRNA positive-strand viruses and is encoded of a single molecule of linear ssRNA of ∼8.6 kb, RT-PCR was developed because it is a suitable detection method for RNA viruses. For the development of the RT-PCR method for detecting GCLV two primers were designed which amplify part of genome which encode gene NABP (nucleic acid binding proteins family). These primers amplify a product of approximately 782 base pairs. The product can be easily visualized on agarose gel electrophoresis. RT-PCR conditions were fully optimized for the detection GCLV in infected plants. This is a report on the development of a method for quick and easy, sensitive and economically reasonable virus detection GCLV. Source

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