Institute of Genetic Engineering and Biotechnology

Alexandria, Egypt

Institute of Genetic Engineering and Biotechnology

Alexandria, Egypt
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Elbayaa R.Y.,Alexandria University | Badr M.H.,Alexandria University | Badr M.H.,King Abdulaziz University | Khalil A.A.,Institute of Genetic Engineering and Biotechnology | Abdelhadi M.,Alexandria University
Drug Research | Year: 2013

A new series of pyrazoline derivatives was designed and synthesized with the objective of developing agents with anti-inflammatory activity together with chemoprevention of hepatobiliary malignancies. The prepared compounds were evaluated for their anti-inflammatory activity using carrageenan-induced granuloma bioassay, using celecoxib as a reference drug. Ulcerogenic effect and acute toxicity profiles (ALD50) for the most active compounds were also determined. Compound 5c was proved to display anti-inflammatory activity better than celecoxib. Compounds 4b, 5d, 5c and 8 were found to be safer than indomethacin with respect to ulcerogenic effect and were well tolerated by the experimental animals with high safety margin (ALD50 >300 mg/Kg). Moreover, histopathological examination was carried out to detect the anti-inflammatory effect of the tested compounds on the livers of carrageenan-injected rats. On the other hand, compounds 4b, 4c, 4d, 5b, 5c, 5d, 6a, 6b, 6c, 6d, 8 and 9 were selected by the NCI to be evaluated for their anticancer activities but none has passed to the 5-dose assay. In addition, the ligand-receptor interactions of the most active compounds with COX-2 were investigated by performing docking studies using Molecular Operating Environment (MOE) version 2008.10. © 2013 Georg Thieme Verlag KG Stuttgart · New York.

Abdellatef A.A.,Institute of Genetic Engineering and Biotechnology | Khalil A.A.,Institute of Genetic Engineering and Biotechnology
Asian Journal of Pharmaceutical Sciences | Year: 2016

Oxidative stress has been implicated in a number of human regeneration and disease processes including atherosclerosis, pulmonary fibrosis, cancer, and different neurodegenerative diseases. The aim of this study was to evaluate the protective effects of Lactobacillus delbrueckii subsp. lactis DSM 20076 (LL-DSM) and Pediococcus acidilactici NNRL B-5627 (PA-NNRL) against the hepatic- and nephro-toxicity of fumonisin B1 (FB1) in FB1-treated rats for an experimental period of 4-weeks. Eighty mature male Sprague-Dawley rats were divided to 12 groups: 1 untreated group; 3 groups fed by a FB1-contaminated diet (50, 100 and 200 mg FB1/kg diet, respectively); 1 group fed orally by LL-DSM (1 ml/d); 1 group fed orally by PA-NNRL (1 ml/d); 3 groups co-administered by FB1-contaminated diet and LL-DSM (1 ml/d), and 3 groups co-administered by FB1-contaminated diet and PA-NNRL (1 ml/d). Malonaldehyde (MDA) nitric oxide, glutathione content, SOD activity, total antioxidant capacity (TAC), total oxidant status (TOS) and oxidative stress index (OSI) were determined. DPA assay was used to assess apoptosis in liver and kidney tissues. The animals fed with FB1-contaminated diet showed a significant increase in oxidative stress markers and DNA fragmentation accompanied with significant decrease in GSH content, SOD activity, and TAC in liver and kidney tissues, especially at high-dosage of FB1 (T200). Probiotics antioxidant strains (LL-DSM and PA-NNRL) relatively succeeded to restore almost all parameters investigated as well as to reduce DNA fragmentation in liver and kidney tissues. As a conclusion, probiotics may induce its protective role via increasing the antioxidant capacity, inhibition of lipid peroxidation, scavenging of free radicals and decreasing DNA lesions in liver and kidney of experimental animals tested. © 2016 Production and hosting by Elsevier B.V.

Khalil A.A.,Institute of Genetic Engineering and Biotechnology | Kabapy N.F.,Institute of Genetic Engineering and Biotechnology | Deraz S.F.,Institute of Genetic Engineering and Biotechnology | Smith C.,Manchester Metropolitan University
Biochimica et Biophysica Acta - Reviews on Cancer | Year: 2011

Heat shock proteins (HSP) are a family of proteins induced in cells exposed to different insults. This induction of HSPs allows cells to survive stress conditions. Mammalian HSPs have been classified into six families according to their molecular size: HSP100, HSP90, HSP70, HSP60, HSP40 and small HSPs (15 to 30 kDa) including HSP27. These proteins act as molecular chaperones either helping in the refolding of misfolded proteins or assisting in their elimination if they become irreversibly damaged. In recent years, proteomic studies have characterized several different HSPs in various tumor types which may be putative clinical biomarkers or molecular targets for cancer therapy. This has led to the development of a series of molecules capable of inhibiting HSPs. Numerous studies speculated that over-expression of HSP is in part responsible for resistance to many anti-tumor agents and chemotherapeutics. Hence, from a pharmacological point of view, the co-administration of HSP inhibitors together with other anti-tumor agents is of major importance in overcoming therapeutic resistance. In this review, we provide an overview of the current status of HSPs in autoimmune, cardiovascular, and neurodegenerative diseases with special emphasis on cancer. © 2011 Elsevier B.V. All rights reserved.

Khalil A.A.,Institute of Genetic Engineering and Biotechnology | Abou-Gabal A.E.,Alexandria University | Abdellatef A.A.,Institute of Genetic Engineering and Biotechnology | Khalid A.E.,Alexandria University
Preparative Biochemistry and Biotechnology | Year: 2015

The genus Fusarium, especially F. verticillioides and F. proliferatum, has been found in several agricultural products worldwide, especially in maize. Regardless the occurrence of symptoms, the presence of Fusarium in maize constitutes an imminent risk due to its ability to produce fumonisins, mycotoxins with proven carcinogenic effect on rats, swine, and equines and already classified as possible carcinogens to humans. The toxicity of incremental levels of fumonisin B1 (FB1), that is, 50, 100, and 200 mg FB1/kg diet, and the role of Lactobacillus delbrueckii subsp. lactis DSM 20076 (LL) and Pediococcus acidilactici NNRL B-5627 (PA) supplementation in counteracting the FB1 effects in intoxicated rats were monitored over a period of 4 weeks. Effects on the feed intake and body weight gain were noticed. A significant (p ≤ 0.05) increase in the level of liver and kidney functions markers and DNA fragmentation was also noticed in rat groups T100 and T200. The lactic acid bacteria (LAB) supplementation could bring back the normal serum biochemical parameters in rats fed on fumonisin B1-contaminated diets (T50 and T100) compared to FB1-treated groups. In rats of high-dosage dietary groups supplemented with LAB (T200-LL and T200-PA), the supplementation reduced the serum activity levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and creatinine by 11.3, 11.9, 32, and 20%, respectively. DNA fragmentations were observed in the rat group treated with 200 mg FB1 after 3 weeks, while fragmentation was noticed in treated groups with 100 and 200 mg FB1 after 4 weeks. No DNA fragmentation was apparent in FB1-treated rats co-administered the LL or PA strain. These results suggest that in male rats consuming diets containing FB1, there is a time- and dose-dependent increase in serum enzyme activities and DNA lesions. Moreover, Lb. delbrueckii subsp. lactis (LL) and P. acidilactici (PA) strains have a protective effect against antigenotoxicity and precancerous lesions. © 2015 Taylor & Francis Group, LLC.

Ghareeb D.A.,Alexandria University | Khalil A.A.,Institute of Genetic Engineering and Biotechnology | Elbassoumy A.M.,Alexandria University | Hussien H.M.,Alexandria University | Abo-Sraiaa M.M.,Institute of Genetic Engineering and Biotechnology
Toxicological and Environmental Chemistry | Year: 2010

Acrylamide (ACR) exerts its toxicity through stimulation of the oxidative stress; yet, its effect on neurotransmitter catabolic enzymes has not been elucidated. We investigated the effects of ACR exposure on brain and hepatic tissues antioxidant enzymes activities and different markers such as, acetylcholinesterase (AChE), nitric oxide (NO), monoamine oxidase (MAO), and lipid profile, and to evaluate the protective effects of garlic against ACR toxicity. Male Sprague-Dawley rats were exposed to ACR (1 mg kg-1 body weight) with or without diet containing 1.5% of garlic powder for 40 days. ACR administration showed a decrease in AChE activity associated with an increase in MAO activity in both brain and hepatic tissues. In addition, ACR administration increased the lipid peroxidation and NO levels of both tissues while decreased the activities of glutathione (GSH), superoxide dismutase, and glutathione-S-transferase (GST). On the other hand, the activities of glutathione peroxidase (GPx) and catalase activities increased as a consequence of GSH depletion after ACR exposure. Finally, ACR exposure increased the brain and liver lipid profile of cholesterol, triglycerides and total lipid, while phospholipids level was decreased. Coadministration of garlic powder with ACR significantly attenuated oxidative stress, MAO activity, and inflammation in brain and hepatic tissues but did not ameliorate AChE activity. In conclusion, our results emphasized the role of garlic as a potential adjuvant therapy to prevent ACR neurotoxicity and hepatotoxicity. © 2010 Taylor & Francis.

Abdeen S.H.,Mansoura University | Abdeen A.M.,Mansoura University | El-Enshasy H.A.,Institute of Genetic Engineering and Biotechnology | El Shereef A.A.,Institute of Genetic Engineering and Biotechnology
Journal of Biological Sciences | Year: 2011

Serum-free cell culture methods are now routinely support mammalian cell growth, a practice adopted for ethical, scientific and safety concerns. The HeLa-S3 cell line is a subclone of the HeLa cell line that can grow in Serum-Free Medium (SFM) as well as suspension cultures. In order to optimize its culturing conditions in SFM, the present study investigated the efficacy of insulin and L-glutamine additives as biotic factors as well as osmotic stress as abiotic factor, all affecting growth kinetics and metabolism. Insulin was used with different concentrations ranging from 10 to 50 mg L-1. It was found that cell growth is dependent on insulin up to a concentration of 25 mg L-1 at which maximum cell number as well as cell viability were achieved. Similarly, L-glutamine was used in the range of 3 to 8 mmol L-1 and was found optimum at 3 mmol L-1. However, osmotic stress using saline solution addition showed that osmolality in the range of 314 to 350 mOsm kg-1 is preferable to cells. The study also showed the successful sequential cell adaptation from adherent culture mode to suspension culture in which cells were able to grow in small clumps of spherical-shaped cells. Based on this cultivation strategy, FfeLa-S3 cells were completely adapted to proliferate suspended in serum-free medium with sustained growth kinetics and physiological properties. © 2011 Asian Network for Scientific Information.

Khalil A.A.,Institute of Genetic Engineering and Biotechnology | Abou-Gabal A.E.,Alexandria University | Elfaramawy A.M.,Institute of Genetic Engineering and Biotechnology | Khaled A.E.,Alexandria University | Abdellatef A.A.,Institute of Genetic Engineering and Biotechnology
Journal of Pure and Applied Microbiology | Year: 2013

Fusarium species are worldwide causal agents of huge damage of storage cereals. Moreover their toxigenic potential is a health risk for both humans and animals. In the present work infected seed samples of maize were collected from local markets in Egypt. Thirteen isolates of Fusarium species were initially identified by phenotype based methods then genotyped using the partial sequence of translation elongation factor-1α (TEF1-α) gene. Furthermore, fumonisin-producing isolates were identified using species-specific primers (PQF5-F/PQF5-R) and (FUM5P2-F/FUM5P2-R) based on partial sequence of FUM1 gene. The results indicated that an amplicon of 60-bp for four isolates identified as F. proliferatum and an amplicon of 70-bp amplicon for three isolates identified as F. Moniliforme were generated. Five strains of lactic acid bacteria (LAB) viz. Lactobacillus delbrueckii subsp. Iactis DSM 20076, Lactobacillus acidophilus DSM 20079, Pediococcus acidilactici NNRL B-5627, Lactobacillus. Sakei LB 706 and Enterococcus faecalis were screened for their ability to inhibit Fusarium isolates growth and/or bind fumonisin B1. Lb. delbrueckii subsp. Iactis DSM 20076 was the most efficient strain at removing fumonisin Bl toxin (76.67 %). However, Lb. acidophilus DSM 20079, Lb. Sakei LB 706 and P. acidilactici NNRL B-5627 showed the greatest inhibitory effect against Fusarium isolates. Therefore the use of LAB as a source of natural antimycotic and antimycotoxin agents showed promising, economical and successful strategy for preserving food products from Fusarium spoilage.

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