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Mita L.,Italian National Institute of Biosystems and Biostructures | Mita L.,Institute of Genetic and Biophysics aBT | Grumiro L.,Italian National Institute of Biosystems and Biostructures | Rossi S.,Institute of Genetic and Biophysics aBT | And 6 more authors.
Journal of Hazardous Materials | Year: 2015

Serratia rubidiae, Pseudomonas aeruginosa and Escherichia coli K12 have been studied for their ability of Bisphenol A removal from aqueous systems and biofilm formation on activated granule carbon. Mathematical equations for biodegradation process have been elaborated and discussed. P. aeruginosa was found the best strain to be employed in the process of Bisphenol A removal. The yield in BPA removal of a P. aeruginosa biofilm grown on GAC and operating in a fluidized bed reactor has been evaluated. The results confirm the usefulness in using biological activated carbon (BAC process) to remove phenol compounds from aqueous systems. © 2015 Elsevier B.V. Source

Errico S.,Italian National Institute of Biosystems and Biostructures | Errico S.,The Second University of Naples | Bianco M.,Italian National Institute of Biosystems and Biostructures | Mita L.,Italian National Institute of Biosystems and Biostructures | And 14 more authors.
Food Chemistry | Year: 2014

A method based on solid-phase extraction followed by liquid chromatography, coupled to UV-visible and fluorescence spectrophotometry, has been developed for determination of bisphenol A (BPA) in canned tomatoes. The limit of quantification (LOQ) of the procedure used is 0.03 μM (0.26 μg BPA/kg tomato). For each of three different tomato based products (peeled, cherry and concentrated paste), 16 samples belonging to six commercial brands, retailed in Italian markets, were tested for migration of BPA epoxy-coating cans. All the tomato samples exhibited migration levels below 0.4 μg/kg, while samples subjected to heating process and/or can's damage by denting, exhibited a significant increase in the migration levels. In any case, no sample contained BPA exceeding the European Union limit for migration, set at 600 μg/kg of food. By comparing the results for each brand, no relevant difference in BPA concentration was found depending on the kind of tomato products. © 2014 Elsevier Ltd. All rights reserved. Source

Nicolucci C.,The Second University of Naples | Nicolucci C.,Messina University | Rossi S.,Institute of Genetic and Biophysics aBT | Menale C.,The Second University of Naples | And 9 more authors.
Analytical and Bioanalytical Chemistry | Year: 2013

A selective and highly sensitive liquid chromatography-tandem mass spectrometry method has been developed and validated for determination of Bisphenol A (BPA) in human urine using labeled d6-BPA as internal standard. BPA was purified from human urine by affinity chromatography on solid extraction AFFINIMIP® Bisphenol A cartridges, based on molecularly imprinted polymers. After purification, the samples were analyzed on a Phenomenex Kinetex 100 × 4.6 mm, 2.6 μm particle PFP reversed-phase HPLC column, coupled to a triple quadrupole mass spectrometer by an electrospray ion source. Analyses were performed in the multiple reaction monitoring mode and negative ionization; the product ions at 133.2 and 212.1 m/z for BPA and at 138.2 and 215.0 m/z for d6-BPA were monitored to assess unambiguous identification. The linearity of the detector response was verified in human urine over the concentration range 0.100-200 ng/mL. The detection limit was calculated as 0.03 ng/mL and the limit of quantification of the method is 0.10 ng/mL. This LC/ESI-MS/MS method was in-house validated evaluating specificity, trueness, within-day and between-days precision. The mean recoveries of BPA from spiked urine samples were higher than 94 % and good reproducibility (relative standard deviations ≤ 8.1 %) was observed. The developed method was applied to a pilot study involving 105 children, aged from 6 to 14 years (16 normal weight and 89 obese children), from the Regione Campania (Southern Italy). The aim of this study was to determine the concentrations of BPA in urine of children and possible correlations with childhood obesity. © 2013 Springer-Verlag Berlin Heidelberg. Source

Menale C.,The Second University of Naples | Menale C.,Italian National Institute of Biosystems and Biostructures | Nicolucci C.,Italian National Institute of Biosystems and Biostructures | Nicolucci C.,Institute of Genetic and Biophysics aBT | And 11 more authors.
Journal of Molecular Catalysis B: Enzymatic | Year: 2012

The oxidative biodegradation of 2- and 4-chlorophenol (CPs) catalyzed by immobilized laccase from Trametes versicolor was conducted in a bed reactor. The effects of reaction conditions (reaction time, dosage of immobilized enzyme, flow rate, pH value and temperature, initial concentration) on CPs removal efficiency (RE) were investigated. By using in the immobilization solution an enzyme dosage of 2.5mgfreeenzgcarrier-1, the maximum ratio of laccase to PAN-beads is resulted 0.25mgimmenzgcarrier-1. RE values greater than 70% were obtained when 40 mL of 1 mM substrate solution were circulated for 90 min at 75 mL min -1 in the bed reactor filled with 12 g of laccase-PAN beads. The optimal pH for CPs biodegradation was 5 and it was not related to substituent position on aromatic ring. Experiments with immobilized laccase demonstrated good removal at high initial substrate concentrations and high thermal stability. The affinity of 2-CP was higher than that of 4-CP. Finally, the 2-CP was also biodegraded as tertiary mixtures with 2,3,4,5- tetrachlorophenol (TCP) and pentachlorophenol (PCP). The presence of TCP and PCP decreased the removal efficiency of 2-CP. The order of the relative degrading capacity becomes TCP > PCP > 2-CP. © 2012 Elsevier B.V. Source

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