Research Institute of General Pathology and Pathophysiology

Moscow, Russia

Research Institute of General Pathology and Pathophysiology

Moscow, Russia
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Alchinova I.B.,Research Institute of General Pathology and Pathophysiology | Yakovenko E.N.,Research Institute of General Pathology and Pathophysiology | Sanadze A.G.,Municipal Clinical Hospital N851 | Karganov M.Yu.,Research Institute of General Pathology and Pathophysiology
Klinichescheskaya Laboratornaya Diagnostika | Year: 2016

The article considers interaction of nano-particles of titan oxide and blood serum of patients with verified diagnoses ofmyasthenia" and "allergy". The analysis of solution of nano-powder of titan oxide using technique of laser correlated spectrometry demonstrated that main input into light scattering is made by particles with radius of 4.64-8.42 nm. The data concerning dimensions of particles received with laser correlated spectrometry matches data of analysis of these particles with various physical techniques. The nano-particles of titan oxide stabilized with phosphoric acid at interaction with blood serum cause development and sedimentation of complexes with albumins and globulins. The interaction of nano-particles of titan oxide with blood serum of patients with myasthenia results in elimination from it the significant percentage (40%) of circulating immune complexes. In blood serum of patients with bronchial asthma, nano-particles of titan oxide bind proteins of small size mainly.


Igon'kina S.I.,Research Institute of General Pathology and Pathophysiology | Vetrile L.A.,Research Institute of General Pathology and Pathophysiology | Kukushkin M.L.,Moscow State University
Bulletin of Experimental Biology and Medicine | Year: 2017

The study focuses on induction of autoantibodies directed against neurotransmitter norepinephrine during neuropathic pain syndrome and on the effect of immunization with norepinephrine-protein conjugated antigen on the development of this syndrome. The formation of anti-norepinephrine antibodies aggravated and prolonged neuropathic pain. © 2017 Springer Science+Business Media New York


Don E.S.,Research Institute of General Pathology and Pathophysiology | Tarasov A.V.,NPF Materia Medica Holding | Epshtein O.I.,Research Institute of General Pathology and Pathophysiology | Tarasov S.A.,NPF Materia Medica Holding
Klinichescheskaya Laboratornaya Diagnostika | Year: 2017

The sphere of application of biomarkers is expanding every year and already comprises their using as indicator of presence or absence of disease, response to therapy, efficiency of medications or pre-clinical model of diagnostic parameter and even participant of process of search of mechanism of effect of medications. Hence, it is impossible to overestimate significance of studying of biomarkers. The article is dedicated to systematization and structuring of present information concerning biomarkers, starting from primary screening and completing with validation of chosen molecule or characteristic.


Khlebnikova N.N.,Laboratory of General Pathology of the Nervous System | Orshanskaya E.V.,Research Institute of General Pathology and Pathophysiology | Narkevich V.B.,V V Zakusov Research Institute Of Pharmacology | Kudrin V.S.,Research Institute of General Pathology and Pathophysiology | Krupina N.A.,Laboratory of General Pathology of the Nervous System
Bulletin of Experimental Biology and Medicine | Year: 2017

The levels of monoamines and their metabolites in brain structures of adult (3-month-old) rats with emotional and motivational disorders induced by inhibitors of dipeptidyl peptidase 4 (DPP-4; EC 3.4.14.5) diprotin A and sitagliptin on weeks 2-3 of postnatal development (postnatal days 5-18) were studied by HPLC with electrochemical detection. A significant decrease in the level of serotonin metabolite, 5-hydroxyindoleacetic acid, and a pronounced tendency towards reduced serotonin level were detected in the striatum of rats in both study groups. In adult rats treated with diprotin A during the neonatal period, a tendency towards activation of dopamine metabolism was observed (judging from DOPAC/DA ratio). The levels of monoamines and their metabolites in the frontal cortex, hypothalamus, and amygdala remained unchanged. The findings suggest that administration of DPP-4 inhibitors during the neonatal period induces long-term dysfunction of the serotonergic and dopaminergic systems of the brain. © 2017 Springer Science+Business Media, LLC


Kireev R.A.,Research Institute of General Pathology and Pathophysiology | Tresguerres A.C.F.,Complutense University of Madrid | Garcia C.,Complutense University of Madrid | Borras C.,University of Valencia | And 4 more authors.
Biogerontology | Year: 2010

There is now a large body of evidence suggesting that the decline in ovarian function with menopause is associated with spontaneous increases in pro-inflammatory cytokines. On the other hand, oxidative stress has been implicated in the pathogenesis of several alterations due to menopause, and can arise through the increased production of lipid peroxides (LPO) and/or a deficiency of antioxidant defense. The aim of the present study was to investigate the effect of aging and ovariectomy on various physiological parameters related to inflammation and oxidative stress in livers obtained from old female rats and the influence of chronic exogenous administration of estrogens, phytoestrogens and growth hormone on these. Thirty-six female Wistar rats of 22 months of age were used in the present study. Twelve of them remained intact, and the other 24 had been ovariectomized at 12 months of age. Intact animals were divided into two groups and treated for 10 weeks with GH or saline, and ovariectomized animals were divided into four groups and treated for the same time with GH, estrogens, phytoestrogens or saline. A group of 2 month old intact female rats was used as young control. Protein expression of iNOS, HO-1, IL-6, TNFa, and IL-1b were determined by Western blot analysis. The levels of NOx, LPO, TNFα, IL-1β, IL-6 and IL-10 were determined in different fractions of the liver. Levels of LPO in the liver homogenates as well as iNOS protein expression and NOx levels were increased in old rats as compared to young animals; this effect was more evident in ovariectomized animals. Pro-inflammatory cytokines TNF-α, IL-1β and IL-6 were significantly increased and anti-inflammatory IL-10 decreased during ageing and after ovariectomy. Aging also significantly increased expression of HO-1 protein and ovariectomized rats showed an additional increase. Hormonal administration to the ovariectomized groups decreased NOx, LPO levels and pro-inflammatory cytokines as compared with untreated rats. Significant rise in IL-10 and reductions in the iNOS, IL-6, TNFα and IL-1β, proteins expression were also found. Oxidative stress and inflammation induced during aging in the liver are more marked in cas trated than in intact old females. Administration of the different hormonal replacement therapies was able to inhibit the induction of proinflammatory cytokines and iNOS, decreased the levels of oxidative stress markers and had therapeutic potential in the prevention of liver injury. .


Saakyan S.V.,Helmholtz Moscow Research Institute of Eye Diseases | Amiryan A.G.,Helmholtz Moscow Research Institute of Eye Diseases | Tsygankov A.Yu.,Helmholtz Moscow Research Institute of Eye Diseases | Loginov V.I.,Research Institute of General Pathology and Pathophysiology | Burdennyi A.M.,Research Institute of General Pathology and Pathophysiology
Arkhiv Patologii | Year: 2014

The study investigated the association of the ABCB1 C3435T polymorphism gene with the risk for uveal melanoma (UM). Genotyping was carried out using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. All the tumors under study were histologically verified. The patients with UM were found to have a high frequency of predisposing alleles and genotypes of this polymorphism in the patients with UM. The CC genotype of the ABCB1 C3435T polymorphism was shown to be associated with the increased risk of UM (OR=8.5;p=7·10-4). A significant correlation was first found between the frequency of the CC genotype of the ABCB1 C3435T polymorphism and the height of a tumor and degree of its pigmentation (p=0.0391 and p=0.0381, respectively, Fisher's test). A marginal association was first shown between the CC genotype of the ABCB1 C3435T polymorphism and the vascular state and histological type.


PubMed | Research Institute of General Pathology and Pathophysiology and Federal Medical Biological Agency
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

Antiviral activity of Ergoferon was studied in vitro on an experimental model of rotavirus infection in MA-104 cell line. In infected cells treated with Ergoferon, rotavirus titer was shown to decrease by 83 and 90% in comparison with cells treated with solvent used for Ergoferon preparation (p<0.05) and distilled water (p<0.05), respectively. These findings demonstrate high anti-rotavirus activity of Ergoferon.


Karpova M.N.,Research Institute of General Pathology and Pathophysiology | Kuznetsova L.V.,Research Institute of General Pathology and Pathophysiology | Zin'kovskii K.A.,Tver State Medical Academy | Klishina N.V.,Research Institute of General Pathology and Pathophysiology
Bulletin of Experimental Biology and Medicine | Year: 2016

We studied anticonvulsant effects of combined treatment with citicoline, a nootropic substance with neuroregenerative and neuroprotective activities, and valproate, an antiepileptic agent widely used in the treatment of epilepsy, on the model of pentylenetetrazole-induced (75 mg/kg, intraperitoneally) acute generalized convulsions in male Wistar rats. Combined treatment with citicoline and valproate in minimum effective doses (70 and 300 mg/kg, respectively) potentiated the anticonvulsant properties of both agents. © 2016 Springer Science+Business Media New York


PubMed | Research Institute of General Pathology and Pathophysiology and Tver State Medical Academy
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

We studied anticonvulsant effects of combined treatment with citicoline, a nootropic substance with neuroregenerative and neuroprotective activities, and valproate, an antiepileptic agent widely used in the treatment of epilepsy, on the model of pentylenetetrazole-induced (75 mg/kg, intraperitoneally) acute generalized convulsions in male Wistar rats. Combined treatment with citicoline and valproate in minimum effective doses (70 and 300 mg/kg, respectively) potentiated the anticonvulsant properties of both agents.


Alchinova I.B.,Research Institute of General Pathology and Pathophysiology | Khlebnikova N.N.,Research Institute of General Pathology and Pathophysiology | Karganov M.Y.,Research Institute of General Pathology and Pathophysiology
Human Physiology | Year: 2015

Information value of the radioadaptive response test was assessed during functional evaluation of pilots in the course of adaptation to flight factors. The functional evaluation was performed at the system level using cardiorespiratory parameters (spirometry and arterial cardiography), at the cellular level using individual sensitivity to ionizing radiation (a radioadaptive response (RAR) test on lymphocytes of peripheral blood), and at the molecular level using the direction of metabolic shifts (laser correlation spectroscopy of biological liquids). Changes in molecular composition of the blood serum and cardiorespiratory functions were compared depending on individual radiosensitivity of pilots with different flight hours. The incidence and strength of RAR in lymphocytes decreased with an increase in flight hours. In light scattering spectra, the input of particles with small hydrodynamic radii (smaller than 11 nm) decreased and that of large particles (larger than 200 nm) increased. The total power of the heart rate variability (HRV) spectrum decreased, whereas the value of stress index increased. Breathing test through a mask raised peripheral systolic and diastolic blood pressure in pilots without RAR. Moreover, it reduced the total spectrum of variability of systolic pressure and input of very low frequencies. In the group of pilots with RAR, the relative input of low frequencies into HRV increased and that of high frequencies decreased, the blood pressure not being altered. Decreased input of small particles and increased input of large particles in light scattering, when compared to the baseline values, were found only in pilots with RAR, which suggests shifts in the metabolism and humoral immunity. It could be hypothesized that the level of individual radiosensitivity reflects the general resistance of the body to negative factors of the environment. © 2015, Pleiades Publishing, Inc.

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