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Sreekanth T.V.M.,Dongguk University | Nagajyothi P.C.,Dongguk University | Prasad T.N.V.K.V.,Institute of Frontier Technology | Duk Lee K.,Dongguk University
Current Nanoscience | Year: 2013

The present study deals with the green synthesis of silver nanoparticles (AgNPs) using Citrus reticulata juice as the reducing agent. The reaction carried out at room temperature (25°C) and at 55°C. The AgNPs were characterized by UV-Vis, TEM, XRD, AFM and FTIR measurements. The produced AgNPs were also evaluated for their increased antibacterial activities with tetracycline and penicillin against Gram-positive and Gram-negative bacteria. In this study, combination of AgNPs (at 55°C) and penicillin showed high inhibition zone in E. coli (18 mm) compared to that of AgNPs (at 25°C) and tetracycline. The cytotoxic effects of AgNPs from the Citrus reticulate juice on B16/ F10 melanoma cell line were evaluated by MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) assay. MTT assay showed a minimum cell inhibition (14.83%) at 0.125 mM (AgNPs at 25°C) and a maximum cell inhibition (31.29%) was observed at 1 mM (AgNPs at 55°C). © 2013 Bentham Science Publishers.


Sudhamani H.,Andhra University | Thaslim Basha S.K.,Andhra University | Muni Chandra Reddy S.,Andhra University | Sreedhar B.,Vikrama Simhapuri University | And 2 more authors.
Research on Chemical Intermediates | Year: 2016

Abstract: Synthesis of a new series of sulfonamide and carbamate derivatives of 7-azaindole and evaluation of their anti-TMV and anti-oxidant activities are reported. Their chemical structures were characterized by IR, 1H and 13C NMR, mass and HRMS spectral analysis. The biological assay revealed that the compounds 4-nitrophenyl 1H-pyrrolo[2,3-b]pyridine-1-carboxylate (9g) and 4-nitrobenzyl 1H-pyrrolo[2,3-b]pyridine-1-carboxylate (9h) exhibited high inhibition against tobacco mosaic virus and abstraction of free radicals. Graphical Abstract: [Figure not available: see fulltext.] © 2016 Springer Science+Business Media Dordrecht


Sudhakar P.,Institute of Frontier Technology | Latha P.,Institute of Frontier Technology | Babu P.R.,Agricultural Research Station | Sujatha K.,Institute of Frontier Technology | Reddy K.R.,Institute of Frontier Technology
Indian Journal of Plant Physiology | Year: 2012

Global warming predicted to affect food security, especially rice, which is stable food crop as it is vulnerable to high temperature. In the present study, a novel temperature induction response (TIR) technique was adapted for identifying highly thermotolerant rice genotypes. This approach of TIR involves, first the identification of challenging temperature and induction temperature and later standardizing them before being used for screening the material for intrinsic stress tolerance. A lethal temperature of 55°C for 2 hours and induction treatment from 36°C-52°C for 5 hours was standardized in the laboratory. Using this standardized TIR protocol, highly thermotolerant rice genotypes were screened from 72 rice germplasm. Sufficient genotypic variability was noticed from zero level to 100% level of tolerance. Among the genotypes, NLR-145 showed highest thermotolerance in terms of 100 per cent seedlings survival and no reduction in root and shoot growth. NLR-40066, NLR-40070 and NLR-40050 also showed higher thermotolerance in terms of 90% seedlings survival and no reduction in root and shoot growth. This study revealed that TIR technique can be used for identification of thermotolerant rice genotypes. The identified rice varieties can be used as donor source for developing high temperature tolerant rice genotypes to withstand future temperature rise.


Sudhamani H.,Sri Venkateswara University | Thaslim Basha S.K.,Sri Venkateswara University | Adam S.,Institute of Frontier Technology | Madhusudhana S.,Sri Venkateswara University | And 2 more authors.
Research on Chemical Intermediates | Year: 2016

Abstract: A series of new urea and thiourea derivatives of lopinavir intermediate 7a–k was designed and synthesized from lopinavir intermediate by treating various biopotent isocyanates and isothiocyanates in the presence of base, 1,4-dimethyl piperazine, and their biological activity was evaluated . The bio-assay revealed that the compounds exhibited moderate to good antimicrobial and antioxidant activities; particularly, 7k showed high activity against Staphylococcus aureus. Further, to know the binding interactions of the title products with protease enzyme, molecular studies were performed, which revealed that 7j exhibited high binding affinity towards the enzyme cysteine protease from Staphylococcus aureus. The results from in vitro studies revealed that they can be used as effective antimicrobial and antioxidant agents. Furthermore, molecular docking studies shown very good interaction. Graphical Abstract: [Figure not available: see fulltext.] © 2016 Springer Science+Business Media Dordrecht

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