Institute of Forensic Science of Ministry of Justice

Shanghai, China

Institute of Forensic Science of Ministry of Justice

Shanghai, China
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Chen C.,Institute of Forensic Science of Ministry of Justice | Chen C.,Centers for Disease Control and Prevention | Chen C.,Fudan University | Yan H.,Institute of Forensic Science of Ministry of Justice | And 2 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2012

A fast method for the quantitative determination of penicillin G PEN G, penicilloic acid and penilloic acid in blood with ultra performance liquid chromatography-electrospray tandem mass spectrometry was developed. A simple deproteinization of the blood was used with a mixed solution of acetonitrile and water 4: I, v/v as extraction solvent. The blood extract was directly injected onto an LC column. The chromatographic separation of the components was performed on a BEH C18 column 50 mm × 2.1 mm, 1.7 μm using acetonitrile and water containing 0.1% formic acid. The mass spectrometer was operated in positive electrospray ion mode. Finally, the analysis was carried out with multiple reaction monitoring MRM mode. The limits of detection LODs for these three compounds were in the range of 0.1 to 2.0 ng/mL aad the limits of quantification LOQs in the range of 0.5 to 5.0 ng/mL. Within the linear range, the correlation coefficients r of PEN G and its metabolites were all more than 0. 997 4. Accuracies for these targeted compounds were ranged from 92.3% to 105.5%, and the within-day precisions were less than 10%. The stabilities of the components were evaluated in the temperature range from 18 to - 80 °C, and the mass concentration of penicillin G was decreased significantly with the extensions of storage temperature and storage time. Biological samples of the rats medicated with PEN G were analyzed using the developed method. The results show that PEN G can just be detected at 0.5 h after administration. However; the detection time limitation of penicilloic acid can be extended to 36 h. The established method has been further expanded for the applicability of forensic identification; and has a reference value for the detection of penicillin G residue in food. © 2010 Editorial Office of Chinese Journal of Chromatography, Dalian Institute of Chemical Physics, CAS.


PubMed | Institute of Forensic Science of Ministry of Justice
Type: Journal Article | Journal: Se pu = Chinese journal of chromatography | Year: 2010

The fingerprint of Kweichow Moutai liquor was established by gas chromatography-mass spectrometry (GC-MS) and the similarity of fingerprints was evaluated by the fingerprint similarity calculation software designed by Zhejiang University based on included angle cosine. One milliliter of liquor sample was mixed with ten microliter of 2% n-pentyl acetate solution used as internal standard. One microliter of the prepared sample was injected into a GC-MS. The separation was performed on an HP-INNOWAX 19091N-113 capillary column. The precision and repeatability of the method were good as the relative standard deviation (RSD) of intrabatch was less than 5%. A total of 35 characteristic components of Kweichow Moutai liquor were identified. The fingerprints of 38 batches of Kweichow Moutai, 5 Moutai-flavor liquors produced by Kweichow Moutai Company Limited, the same manufacturer as Kweichow Moutai, and 12 other brand liquors were compared in characteristic components and similarity. The results demonstrated that different batches of Kweichow Moutai had good similarity (> or = 0.9), and Kweichow Moutai was differentiated from the liquors of different alcohol contents and flavors, but it was poorly distinguished from the Moutai-flavor liquors by fingerprint similarity calculation software. Therefore, only in combining characteristic components and fingerprint similarity results, Kweichow Moutai can be distinguished from other liquors. The established method offers technical basis for the identification of Kweichow Moutai.


PubMed | Institute of Forensic Science of Ministry of Justice
Type: Journal Article | Journal: Se pu = Chinese journal of chromatography | Year: 2012

A fast method for the quantitative determination of penicillin G (PEN G) , penicilloic acid and penilloic acid in blood with ultra performance liquid chromatography-electrospray tandem mass spectrometry was developed. A simple deproteinization of the blood was used with a mixed solution of acetonitrile and water (4:1, v/v) as extraction solvent. The blood extract was directly injected onto an LC column. The chromatographic separation of the components was performed on a BEH C18 column (50 mm x 2.1 mm, 1.7 microm) using acetonitrile and water containing 0.1% formic acid. The mass spectrometer was operated in positive electrospray ion mode. Finally, the analysis was carried out with multiple reaction monitoring (MRM) mode. The limits of detection (LODs) for these three compounds were in the range of 0.1 to 2.0 ng/mL and the limits of quantification (LOQs) in the range of 0.5 to 5.0 ng/mL. Within the linear range, the correlation coefficients (r) of PEN G and its metabolites were all more than 0.9974. Accuracies for these targeted compounds were ranged from 92.3% to 105.5%, and the within-day precisions were less than 10%. The stabilities of the components were evaluated in the temperature range from 18 to 80 degrees C, and the mass concentration of penicillin G was decreased significantly with the extensions of storage temperature and storage time. Biological samples of the rats medicated with PEN G were analyzed using the developed method. The results show that PEN G can just be detected at 0.5 h after administration. However, the detection time limitation of penicilloic acid can be extended to 36 h. The established method has been further expanded for the applicability of forensic identification, and has a reference value for the detection of penicillin G residue in food.

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