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Caceres P.J.,Institute of Food Science | Martinez-Villaluenga C.,Institute of Food Science | Amigo L.,Institute of Food Science Research | Frias J.,Institute of Food Science
Food Chemistry | Year: 2014

Germinated brown rice (GBR) is considered a healthy alternative to white rice in the fight against chronic diseases. As the functional quality of GBR depends on genotype and germination conditions, the objectives were to identify suitable Ecuadorian brown rice cultivars and optimal germination time and temperature to maximise γ-aminobutyric acid (GABA), total phenolics compounds (TPC) and antioxidant activity of GBR. Regression models for the prediction of phytochemical composition and antioxidant activity in GBR were also obtained. Germination improved GABA, TPC and antioxidant activity in all cultivars. Maximum GABA and antioxidant activity were attained at 34 C for 96 h, while the highest TPC was found at 28 C for 96 h in all cultivars. GBR cv. GO displayed the highest antioxidant activity and cv. 15 was the most effective at accumulating GABA and TPC in the optimal germination conditions. Therefore, Ecuadorian GBR could be used for the preparation of functional foods serving as preventative strategies in combating chronic diseases. © 2013 Elsevier Ltd. All rights reserved. Source

Solis M.-T.,CSIC - Biological Research Center | Rodriguez-Serrano M.,CSIC - Biological Research Center | Meijon M.,University of Oviedo | Canal M.-J.,University of Oviedo | And 3 more authors.
Journal of Experimental Botany | Year: 2012

Stress-induced plant cell reprogramming involves changes in global genome organization, being the epigenetic modifications key factors in the regulation of genome flexibility. DNA methylation, accomplished by DNA methyltransferases, constitutes a prominent epigenetic modification of the chromatin fibre which is locked in a transcriptionally inactive conformation. Changes in DNA methylation accompany the reorganization of the nuclear architecture during plant cell differentiation and proliferation. After a stress treatment, in vitro-cultured microspores are reprogrammed and change their gametophytic developmental pathway towards embryogenesis, the process constituting a useful system of reprogramming in isolated cells for applied and basic research. Gene expression driven by developmental and stress cues often depends on DNA methylation; however, global DNA methylation and genome-wide expression patterns relationship is still poorly understood. In this work, the dynamics of DNA methylation patterns in relation to nuclear architecture and the expression of BnMET1a-like DNA methyltransferase genes have been analysed during pollen development and pollen reprogramming to embryogenesis in Brassica napus L. by a multidisciplinary approach. Results showed an epigenetic reprogramming after microspore embryogenesis induction which involved a decrease of global DNA methylation and its nuclear redistribution with the change of developmental programme and the activation of cell proliferation, while DNA methylation increases with pollen and embryo differentiation in a cell-type-specific manner. Changes in the presence, abundance, and distribution of BnMET1a-like transcripts highly correlated with variations in DNA methylation. Mature zygotic and pollen embryos presented analogous patterns of DNA methylation and MET1a-like expression, providing new evidence of the similarities between both developmental embryogenic programmes. © 2012 The Author(s). © 2012 The Authors. Source

Hurtado-Benavides A.,University of Narino | Dorado D.A.,University of Narino | Sanchez-Camargo A.D.P.,Institute of Food Science Research
Journal of Supercritical Fluids | Year: 2016

In the present work, the extraction of oil from roasted coffee beans using supercritical carbon dioxide (SCCO2) under different conditions of pressure (15-30 MPa) and temperature (40-60°C) was studied. A central composite experimental design was employed in order to establish the effect of these parameters on the yield and fatty acids composition that was determined by gas chromatography with flame ionization detector (GC-FID). The aroma was analyzed by headspace solid phase microextraction (HS-SPME). The optimum oil yield was 8.9%, obtained at 33.1 MPa and 35.9°C. The main fatty acids identified were palmitic (46.1%), linoleic (32.9%), oleic (8.0%), stearic (6.6%) and arachidic (1.9%). The volatile compounds of the coffee oil belong mainly to the family of furans and pyrazines, which maintain the particular features of the roasted coffee. This fact makes the coffee oil attractive to be used in the food and/or cosmetic industry. © 2016 Elsevier B.V. All rights reserved. Source

Miralles B.,Institute of Food Science Research | Mengibar M.,Complutense University of Madrid | Harris R.,Complutense University of Madrid | Heras A.,Complutense University of Madrid
Food Chemistry | Year: 2011

A colorimetric method previously described for the determination of chitosan has been evaluated because lack of linearity had been observed at certain concentrations. Calibration curves of varied-characteristic chitosans, recovery studies and chitosan quantification in seven commercial dietary supplements have been performed. Some analysis conditions including the solvent of the samples have been studied and optimised. Different data combinations have been checked in order to select the widest range of concentrations where no serial correlation was found. With the selected conditions the method is linear, reproducible and provides reliable results in the analysis of the chitosan content in capsules. Its selectivity has been proved by the lack of interference with other compounds present in the dietary supplements. But in the case of tablet products, the presence of cellulose and magnesium stearate may produce an underestimation of the chitosan content. © 2010 Elsevier Ltd. All rights reserved. Source

Martos G.,Institute of Food Science Research | Pineda-Vadillo C.,Institute of Food Science Research | Miralles B.,Institute of Food Science Research | Alonso-Lebrero E.,Section of Pediatric Allergy | And 3 more authors.
Journal of Agricultural and Food Chemistry | Year: 2012

Riboflavin binding protein (RfBP) is a minor protein in hen egg; its potential involvement in egg allergy has seldom been studied. The aim of this work was to investigate the IgE binding capacity of RfBP before and after simulated gastrointestinal digestion. It was shown that digestion of RfBP mainly occurred during the gastric phase. The protein fragments resulting from the subsequent duodenal phase remained linked through disulfide bonds. Both the intact protein and its digests were subjected to inhibition ELISA with sera obtained from patients allergic to egg. The results revealed significant IgE binding to intact RfBP, whereas the digests showed reduced but substantial IgE binding levels, with serum-to-serum variability. The RfBP digests were then subjected to immunoblot with allergic patients' sera, and the IgE-reactive peptides were further analyzed by MALDI-TOF/TOF mass spectrometry for sequence determination. The RfBP sequence 41-84 was identified as a novel IgE binding peptide in patients allergic to egg. © 2012 American Chemical Society. Source

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