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Fort Lauderdale, FL, United States

Leonard M.T.,University of Florida | Fagen J.R.,University of Florida | Davis-Richardson A.G.,University of Florida | Davis M.J.,Institute of Food and Agricultural science | Triplett E.W.,University of Florida
Standards in Genomic Sciences | Year: 2012

Liberibacter crescens BT-1, a Gram-negative, rod-shaped bacterial isolate, was previously re-covered from mountain papaya to gain insight on Huanglongbing (HLB) and Zebra Chip (ZC) diseases. The genome of BT-1 was sequenced at the Interdisciplinary Center for Biotechnolo-gy Research (ICBR) at the University of Florida. A finished assembly and annotation yielded one chromosome with a length of 1,504,659 bp and a G+C content of 35.4%. Comparison to other species in the Liberibacter genus, L. crescens has many more genes in thiamine and es-sential amino acid biosynthesis. This likely explains why L. crescens BT-1 is culturable while the known Liberibacter strains have not yet been cultured. Similar to Candidatus L. asiaticus psy62, the L. crescens BT-1 genome contains two prophage regions.

Miller D.L.,Institute of Food and Agricultural science | Thetford M.,Institute of Food and Agricultural science | Dupree J.,Institute of Food and Agricultural science | Atwood L.,University of New Hampshire
Journal of Coastal Research | Year: 2014

Influence of seasonal changes and shifting substrate on survival of restoration plantings of Schizachyrium maritimum (Gulf bluestem) on Santa Rosa Island, Florida. Environmental gradients on barrier islands are modified by the loss of dunes as a result of hurricane overwash. The success of restoration plantings in posthurricane landscapes is determined by abiotic conditions, including seasonal and spatial gradients of wind speed and substrate transport encountered before dune redevelopment. The response of Schizachyrium maritimum, a dominant, sand-trapping, dune-forming grass of the northern Gulf Coast to month of planting, sand manipulation, and plant spacing was investigated on Santa Rosa Island, Florida. This grass is also a valuable food for endangered beach mice. Seasonal plantings were initiated in 4 months (November 2005, February 2006, June 2006, and August 2006) at each of six sites. For each site and each planting date, plots were split by plant spacing (30 or 45 cm between plants) and again by two sand manipulation regimes (natural sand accumulation or sand level at 3 cm). Sand surface levels were determined and manipulated every 2 weeks. Plant survival and growth were recorded September 2006 and July 2007. Plant survival and growth were greatest for summer plantings, particularly June plantings. Sand burial by natural accumulation did not result in plant death. Most plant death, particularly for November and February plantings, was associated with root exposure and sand loss around transplants. Closer spacing appeared to slow sand loss and plant death for November plantings. Planting of S. maritimum should be restricted to summer months, preferably June, and where sand loss can be minimized by use of mulch or other sand stabilizing methods. © 2014 Coastal Education and Research Foundation.

Sandhu A.K.,University of Florida | Gray D.J.,Institute of Food and Agricultural science | Lu J.,Florida A&M University | Gu L.,University of Florida
Food Chemistry | Year: 2011

The objective of this study was to evaluate the effect of exogenous abscisic acid (ABA) on the antioxidant capacity and phenolic content of muscadine grape skins (cvs. Noble and Alachua). ABA was applied on grapes during and after veraison. Average berry weight, total soluble solids ( 0Brix) and pH of the juice from both cultivars were not affected by the ABA treatment. Antioxidant capacity was enhanced by 38% and 18% in treated Noble at the first and second sampling, respectively. High performance liquid chromatography analysis revealed a significant increase in individual anthocyanins in treated Noble grapes at both sampling times. However, increase in the content of ellagic acid, myricetin, quercetin and kaempferol was observed at first sampling only. No effects of ABA treatment were seen in Alachua grapes. Our results indicate that exogenous application of ABA enhances the antioxidant capacity, anthocyanins and phenolic content of muscadine grapes but these effects may vary depending upon the cultivar. © 2010 Elsevier Ltd. All rights reserved.

Davis-Richardson A.G.,Institute of Food and Agricultural science | Triplett E.W.,Institute of Food and Agricultural science
Diabetologia | Year: 2015

Several lines of evidence suggest a role for the gut microbiome in type 1 diabetes. Treating diabetes-prone rodents with probiotics or antibiotics prevents the development of the disorder. Diabetes-prone rodents also have a distinctly different gut microbiome compared with healthy rodents. Recent studies in children with a high genetic risk for type 1 diabetes demonstrate significant differences in the gut microbiome between children who develop autoimmunity for the disease and those who remain healthy. However, the differences in microbiome composition between autoimmune and healthy children are not consistent across all studies because of the strong environmental influences on microbiome composition, particularly diet and geography. Controlling confounding factors of microbiome composition uncovers bacterial associations with disease. For example, in a human cohort from a single Finnish city where geography is confined, a strong association between one dominant bacterial species, Bacteroides dorei, and type 1 diabetes was discovered (Davis-Richardson et al. Front Microbiol2014;5:678). Beyond this, recent DNA methylation analyses suggest that a thorough epigenetic analysis of the gut microbiome may be warranted. These studies suggest a testable model whereby a diet high in fat and gluten and low in resistant starch may be the primary driver of gut dysbiosis. This dysbiosis may cause a lack of butyrate production by gut bacteria, which, in turn, leads to the development of a permeable gut followed by autoimmunity. The bacterial community responsible for these changes in butyrate production may vary around the world, but bacteria of the genus Bacteroides are thought to play a key role. © 2015, The Author(s).

Danyluk M.D.,University of Florida | Danyluk M.D.,Institute of Food and Agricultural science | Friedrich L.M.,University of Florida | Jouquand C.,University of Florida | And 4 more authors.
Food Microbiology | Year: 2011

The presence of . Alicyclobacillus in fruit juices and concentrates poses a serious problem for the juice industry. This study was undertaken to determine the (i) prevalence, concentration, and species of . Alicyclobacillus in tropical and subtropical concentrates; (ii) efficacy of aqueous chlorine dioxide in reducing . Alicyclobacillus spp. spores on tropical and subtropical fruit surfaces; and (iii) fate of and off-flavor production by . Alicyclobacillus acidoterrestris in mango and pineapple juices. One hundred and eighty tropical and subtropical juice concentrates were screened for the presence and concentration of . Alicyclobacillus spp. If found, the species of . Alicyclobacillus was determined by 16S rDNA sequencing and analysis with NCI BLAST. Of these samples, 6.1% were positive for . Alicyclobacillus, and nine . A. acidoterrestris strains and two . Alicyclobacillus acidocaldarius strains were identified. A five-strain cocktail of . Alicyclobacillus spp. was inoculated onto the surface of fruits (grapefruit, guava, limes, mangoes, oranges and pineapple), which were then washed with 0, 50, or 100 ppm aqueous chlorine dioxide. Significant reductions due to chlorine dioxide were only seen on citrus fruits. A five-strain cocktail of . A. acidoterrestris was inoculated into mango and pineapple juices. Microbial populations were enumerated over a 16-day period. Aroma compounds in the juice were analyzed by GC-olfactometry (GC-O) and confirmed using GC-MS. GC-O of mango juice identified previously reported medicinal/antiseptic compounds. GC-O of pineapple juice revealed an unexpected " cheese" off-aroma associated with 2-methylbutyric acid and 3-methylbutyric acid. © 2010 Elsevier Ltd.

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