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Limanskaya O.Yu.,Ukrainian Academy of Sciences | Limanskaya O.Yu.,Institute of Experimental and Clinical Veterinary Medicine
Cytology and Genetics | Year: 2010

The perfect interstrand triplexes that could potentially arise in the proviral DNA of two widespread cattle retroviruses such as bovine leukemia virus (BLV) and bovine immunodeficiency virus (BIV) were determined. The fragments, which formed triplexes at acidic pH, were found in the genomes of both viruses; five fragments were found in BVL and 10 fragments in BIV. One of these fragments (it is localized in the BVL gag gene) might exist like a part of a cruciform structure. Existence of the triplexes was experimentally confirmed by visualization of supercoiled pGEMEX DNA with the use of atomic force microscopy; six fragments with mirror symmetry, which are necessary for formation of intramolecular triplexes, were found. Triplexes represent one of the elements of the signaling mechanisms of the genome function. Maps of triplex location in the cattle retroviral genome were built. © Allerton Press, Inc., 2010.

Brusentsov I.I.,RAS Institute of Cytology and Genetics | Katokhin A.V.,RAS Institute of Cytology and Genetics | Brusentsova I.V.,RAS Institute of Cytology and Genetics | Shekhovtsov S.V.,RAS Institute of Cytology and Genetics | And 9 more authors.
PLoS ONE | Year: 2013

Opisthorchis felineus or Siberian liver fluke is a trematode parasite (Opisthorchiidae) that infects the hepato-biliary system of humans and other mammals. Despite its public health significance, this wide-spread Eurasian species is one of the most poorly studied human liver flukes and nothing is known about its population genetic structure and demographic history. In this paper, we attempt to fill this gap for the first time and to explore the genetic diversity in O. felineus populations from Eastern Europe (Ukraine, European part of Russia), Northern Asia (Siberia) and Central Asia (Northern Kazakhstan). Analysis of marker DNA fragments from O. felineus mitochondrial cytochrome c oxidase subunit 1 and 3 (cox1, cox3) and nuclear rDNA internal transcribed spacer 1 ( ITS1) sequences revealed that genetic diversity is very low across the large geographic range of this species. Microevolutionary processes in populations of trematodes may well be influenced by their peculiar biology. Nevertheless, we suggest that lack of population genetics structure observed in O. felineus can be primarily explained by the Pleistocene glacial events and subsequent sudden population growth from a very limited group of founders. Rapid range expansion of O. felineus through Asian and European territories after severe bottleneck points to a high dispersal potential of this trematode species. © 2013 Brusentsov et al.

Limanskaya O.Y.,Ukrainian Academy of Sciences | Limanskaya O.Y.,Institute of Experimental and Clinical Veterinary Medicine | Fesenko T.N.,Ukrainian Academy of Sciences | Pokrovskiy V.A.,Ukrainian Academy of Sciences | And 4 more authors.
Biomeditsinskaya Khimiya | Year: 2012

Point mutations associated with isoniazid resistance in Mycobacterium tuberculosis (MTB) have been analyzed in codon 315 of the katG gene by conventional polymerase chain reaction (PCR) using primers containing locked nucleic acid (LNA) modified nucleotides. Purity and structure of primers containing 5 LNA monomers of 17 nucleotides in length were characterized by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and a 17-mcr duplex formed by two complementary oligonucleotides was characterized by the method of thermal denaturation. The duplex containing five LNA monomers per each strand was characterized by a higher melting temperature than it was expected using extrapolation of theoretical calculation for nucleotide modification of one strand of the duplex. Detection of any of six possible mutations in katG codon 315 (i.e. discrimination between sensitive and resistant MTB) requires just one PCR employing a set of two primers with one LNA-modified primer; this is an important advantage of oligonucleotides containing LNA over unmodified nucleotides: employment of multiplex PCR would require up to 12 primers. Problems of control of oligonucleotide modification by LNA monomers are discussed.

Korotetsky I.S.,Institute of Microbiology and Virology | Bogoyavlensky A.P.,Institute of Microbiology and Virology | Prilipov A.G.,Russian Academy of Medical Sciences | Usachev E.V.,Russian Academy of Medical Sciences | And 10 more authors.
Voprosy Virusologii | Year: 2010

The F gene fragment of 79 Newcastle disease virus (NDV) strains Isolated from domestic and synanthropic birds in Kazakhstan, Kirghizia, Ukraine, and Russia in 1993 to 2007 was comparatively analyzed. Phylogenetic analysis of test isolates and reference NDV strains obtained from the GenBank was carried out by polymerase chain reaction with subsequent sequencing and comparative analysis of 154-bp nucleotide sequences in the main functional region of the F gene. All newly characterized isolates belong to three NDV genotype VII subgroups: Vila, VIIb, VIId. The results show it necessary to monitor of NDV strains isolated In the CIS countries since the spread of NDV among migratory and synanthropic birds (pigeons, crows, and jackdaws) poses a serious threat to commercial poultry Industry.

Muzyka D.,Institute of Experimental and Clinical Veterinary Medicine | Pantin-Jackwood M.,U.S. Department of Agriculture | Starick E.,Friedrich Loeffler Institute | Fereidouni S.,Friedrich Loeffler Institute | And 2 more authors.
Archives of Virology | Year: 2016

Since the first detection of H15 avian influenza viruses (AIVs) in Australia in 1979, only seven H15 strains have been reported. A new H15 AIV was detected in Ukraine in 2010, carrying the unique HA-NA subtype combination H15N7. This virus replicated efficiently in chicken eggs, and antisera against it reacted strongly with the homologous antigen, but with lower titers when using the reference Australian antigen. The amino acid motifs of the HA cleavage site and receptor-binding site were different from those in the Australian viruses. The new virus, together with an H15 virus from Siberia from 2008, constitutes a new clade of H15 AIV isolates. © 2015, Springer-Verlag Wien.

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