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Pivoriuunas A.,Institute of Experimental and Clinical Medicine | Surovas A.,Institute of Experimental and Clinical Medicine | Borutinskaite V.,Lithuanian Academy of Sciences | Matuzeviccius D.,Vilnius Gediminas Technical University | And 11 more authors.
Stem Cells and Development | Year: 2010

Human dental pulp derived from exfoliated deciduous teeth has been described as a promising alternative source of multipotent stem cells. While these cells share certain similarities with mesenchymal stem-like cells (MSC) isolated from other tissues, basically they are still poorly characterized. In this study, for the first time, a proteomic map of abundantly expressed proteins in stromal cells derived from the dental pulp of human exfoliated deciduous teeth (SHED) was established. We also analyzed proteomic signatures of 2 clonal strains derived from SHEDs by single-cell cloning. The SHEDs were established from enzyme-disaggregated deciduous dental pulp from 6-year-old children. They had typical fibroblastoid morphology and high colony-forming efficiency index (16.4%). Cloning was performed at the second passage using limiting dilution in a 96-well plate (0.3 cell/well). Differentiation assessment revealed strong osteogenic but no adipogenic potential of the SHEDs in either clonal strain. The cells expressed characteristic antigens of MSC-like cells, including CD73, CD90, CD105, CD146, and did not express hematopoietic markers CD14, CD34, and CD45, as assessed with FACS analysis. For proteomic studies, cytosolic and nuclear proteins were analyzed with 2-dimensional gel electrophoresis (2-DE) and identified using matrix-assisted laser desorption/ionization (MALDI)-time of fl ight (TOF)-mass spectrometry (MS). All proteins were identified with high level of confidence (the lowest sequence coverage was 27%). Identification of highly expressed proteins in SHEDs revealed proteomic profiles very similar to that of MSC-like cells derived from other tissues. We also found a high degree of similarity between proteomic signatures of primary SHEDs and clonal cell strains. Thus, our data confirm a close resemblance between SHEDs and MSC-like cells from other tissues and may serve as starting point for creating-comprehensive proteomic maps. © 2010 Mary Ann Liebert, Inc.


Nesteruk T.,Central Clinical Hospital of the Ministry of Interior in Warsaw | Nesteruk M.,Central Clinical Hospital of the Ministry of Interior | Styczynska M.,Polish Academy of Sciences | Barcikowska-Kotowicz M.,Polish Academy of Sciences | And 2 more authors.
Polish Journal of Radiology | Year: 2016

Background: The aim of the study was to evaluate the diagnostic value of two measurement techniques in patients with cognitive impairment – automated volumetry of the hippocampus, entorhinal cortex, parahippocampal gyrus, posterior cingulate gyrus, cortex of the temporal lobes and corpus callosum, and fractional anisotropy (FA) index measurement of the corpus callosum using diffusion tensor imaging. Material/Methods: A total number of 96 patients underwent magnetic resonance imaging study of the brain – 33 healthy controls (HC), 33 patients with diagnosed mild cognitive impairment (MCI) and 30 patients with Alzheimer’s disease (AD) in early stage. The severity of the dementia was evaluated with neuropsychological test battery. The volumetric measurements were performed automatically using FreeSurfer imaging software. The measurements of FA index were performed manually using ROI (region of interest) tool. Results: The volumetric measurement of the temporal lobe cortex had the highest correct classification rate (68.7%), whereas the lowest was achieved with FA index measurement of the corpus callosum (51%).The highest sensitivity and specificity in discriminating between the patients with MCI vs. early AD was achieved with the volumetric measurement of the corpus callosum – the values were 73% and 71%, respectively, and the correct classification rate was 72%.The highest sensitivity and specificity in discriminating between HC and the patients with early AD was achieved with the volumetric measurement of the entorhinal cortex – the values were 94% and 100%, respectively, and the correct classification rate was 97%.The highest sensitivity and specificity in discriminating between HC and the patients with MCI was achieved with the volumetric measurement of the temporal lobe cortex – the values were 90% and 93%, respectively, and the correct classification rate was 92%.Conclusions: The diagnostic value varied depending on the measurement technique. The volumetric measurement of the atrophy proved to be the best imaging biomarker, which allowed the distinction between the groups of patients. The volumetric assessment of the corpus callosum proved to be a useful tool in discriminating between the patients with MCI vs. early AD. © Pol J Radiol.


Szczerbinska K.,Jagiellonian University | Topinkova E.,Charles University | Ceremnych J.,Institute of Experimental and Clinical Medicine | Gindin J.,The Geriatric Institute for Education and Research | Maggi S.,CNR Institute of Neuroscience
European Geriatric Medicine | Year: 2010

Background: Stroke is one of the most disabling conditions in old age. Good quality of care may prevent subsequent disability. Yet, recommendations for stroke care focus mainly on acute phase. Much less consistency exists in later phases when care is delivered by a wide spectrum of providers. The aim of the study was to map current trajectories of stroke patients through different health care settings in three European countries: Czech Republic, Lithuania, and Poland. Methods: The research was conducted within the CLESA project ("Cross-National Determinants of Quality of Life and Health Services for the Elderly") in 2003. It combined qualitative methods of case study questionnaire and expert consensus group. Existing standards of care, accessibility of health and social care services were compared cross-nationally focusing on time factor, professionals involved, administrative and financial components of services. Results: Application of international recommendations for acute care for stroke patients positively affected accessibility of acute care services in all the countries considered in the study. Differences between countries occurred mainly in the post-acute care in the type of institution, professionals involved, eligibility criteria for rehabilitation and financial resources for care. Home care was the most diverse and fragmented service in terms of provider type, organisation and payment system. Conclusions: The evidence-based medicine shaped medical care during early stroke in the Eastern European countries into rather uniform performance resulting in development of acute stroke units. However, higher heterogeneity in the rehabilitation and home care services may come out of inaccuracies in the recommendations and standards of care in later stage after stroke. © 2010 Elsevier Masson SAS and European Union Geriatric Medicine Society.


Pal'chikova N.A.,Institute of Experimental and Clinical Medicine | Kuznetsova N.V.,Institute of Experimental and Clinical Medicine | Selyatitskaya V.G.,Institute of Experimental and Clinical Medicine | Cherkasova O.P.,Institute of Experimental and Clinical Medicine | Kuz'mina O.I.,Institute of Experimental and Clinical Medicine
Bulletin of Experimental Biology and Medicine | Year: 2016

We studied the content of corticosterone and its precursors in the adrenal glands, corticosterone in blood serum and daily urine of rats, and activity of first and second isoforms of 11β-hydroxysteroid dehydrogenase in the liver and kidneys of rats after 15 daily intraperitoneal injections of 0.9% NaCl or glucocorticoid receptor blocker mifepristone in 0.9% NaCl. Daily injections of NaCl reduced the levels of pregnenolone, progesterone, and corticosterone in the adrenal glands, increased corticosterone excretion with urine, enhanced activity of the first isoform of 11β-hydroxysteroid dehydrogenase in the liver and reduction in activity of the second isoform of this enzyme in the kidneys. These changes are typical manifestations of chronic stress. Mifepristone restored pregnenolone content in the adrenal glands and increase in corticosterone concentration in the blood. Under these conditions, activity of the first isoform of 11β-hydroxysteroid dehydrogenase in the liver did not change, and a decrease in activity of the second isoform of the enzyme in the kidneys was less pronounced. The results suggest that mifepristone abolished the stress-mediated increase in activity of the first isoform of 11β-hydroxysteroid dehydrogenase in the liver and reduced local production of glucocorticoid hormones and their metabolic effects in hepatocytes. © 2016 Springer Science+Business Media New York


Potapova O.V.,Institute of Experimental and Clinical Medicine | Kovner A.V.,Institute of Experimental and Clinical Medicine | Anikina A.G.,Institute of Experimental and Clinical Medicine | Cherdantseva L.A.,Institute of Experimental and Clinical Medicine | And 4 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2016

Influenza A/H1N1 A/Tomsk/13/2010 virus registered in Siberia in 2010 proved to be an extremely pathogenic strain. Dynamic study of the topology of this influenza virus strain in the lungs, liver, kidneys, lymph nodes, and great vessels of infected mice was carried out. Influenza A virus was detected by immunohistochemical methods in cells of different histogenesis in all the studied organs throughout the observation period (days 1-30 postinfection), which indicated effective replication and long persistence of influenza A/H1N1 A/Tomsk/13/2010 virus in mammalian cells. © 2016 Springer Science+Business Media New York


Kim L.B.,Institute of Experimental and Clinical Medicine | Shkurupy V.A.,Novosibirsk State Medical University | Putyatina A.N.,Institute of Experimental and Clinical Medicine
Bulletin of Experimental Biology and Medicine | Year: 2016

Activity of MMP and content of TIMP and some proteoglycan components were measured in the liver, lungs, and spleen of BALB/c mice aging 2, 6, and 12 months. The increase in the content of proteoglycan components in mouse organs was associated with age-related changes in MMP activity and TIMP-1 and TIMP-2 levels. The ratio between MMP activity and content of TIMP-1 and TIMP-2 differed in the studied organs. The levels of TIMP-1 and TIMP-2 in mouse blood serum and their concentration in studied organs changed asynchronously with age. They are important regulators that determine MMP activity in the analyzed age periods in mice, which can be determined as periods of growth, development, and ageing. © 2016, Springer Science+Business Media New York.


PubMed | Institute of Experimental and Clinical Medicine
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

Activity of MMP and content of TIMP and some proteoglycan components were measured in the liver, lungs, and spleen of BALB/c mice aging 2, 6, and 12 months. The increase in the content of proteoglycan components in mouse organs was associated with age-related changes in MMP activity and TIMP-1 and TIMP-2 levels. The ratio between MMP activity and content of TIMP-1 and TIMP-2 differed in the studied organs. The levels of TIMP-1 and TIMP-2 in mouse blood serum and their concentration in studied organs changed asynchronously with age. They are important regulators that determine MMP activity in the analyzed age periods in mice, which can be determined as periods of growth, development, and ageing.


PubMed | Institute of Experimental and Clinical Medicine
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

Influenza A/H1N1 A/Tomsk/13/2010 virus registered in Siberia in 2010 proved to be an extremely pathogenic strain. Dynamic study of the topology of this influenza virus strain in the lungs, liver, kidneys, lymph nodes, and great vessels of infected mice was carried out. Influenza A virus was detected by immunohistochemical methods in cells of different histogenesis in all the studied organs throughout the observation period (days 1-30 postinfection), which indicated effective replication and long persistence of influenza A/H1N1 A/Tomsk/13/2010 virus in mammalian cells.

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