Nikiyan H.,Orenburg State University |
Tatlybaeva E.,Orenburg State University |
Rayev M.,Institute of Ecology and Genetics of Microorganisms |
Deryabin D.,Orenburg State University
Current Nanoscience | Year: 2015
The application of gold and carbon labels for the detection of antigen molecules using the atomic force microscopy (AFM) method is described in the current study. Direct AFM visualisation made it possible to observe the “antigen–antibody–gold+protein A” and “antigen–antibody– carbon+protein G” complexes. In addition, it was also possible to estimate the morphology and describe the distribution on a surface that used plastic microwell plates as a substrate. The sensitivity of the method for each conjugate was evaluated and compared to an enzyme-linked immunosorbent assay. The results obtained point to a high sensitivity of the approach, especially when using carbon tags. In addition, certain advantages of carbon tags are indicated, when compared with gold tags, because of their simple detection and unambiguous identification on the collected images. © 2015 Bentham Science Publishers.
Gein S.V.,Institute of Ecology and Genetics of Microorganisms |
Siytchihin A.A.,Russian Academy of Sciences
Bulletin of Experimental Biology and Medicine | Year: 2010
Dynorphin A (1-17) in concentrations of 10-8-10-9 M inhibits phytohemagglutinin (2.5 μg/ml)- induced proliferative response of mononuclear fraction lymphocytes. In mitogen-stimulated cultures, 10 -8 M dynorphin A (1-17) stimulates the production of IL-4, inhibits the production of IL-2, and does not modify the production of IFN-γ. Nonselective -receptor antagonist naloxone and selective antagonist binaltorphimine hydrochloride abolish the inhibitory effects of both dynorphin A concentrations on the lymphocyte proliferative response. On the other hand, evaluation of the effect of -receptor blockade on the production of IL-2 and IL-4 showed that this effect depends on peptide concentration and antagonist type. Hence, the results attest to an important role of -receptors in modulation of functional activity of immune cells. © 2010 Springer Science+Business Media, Inc.
Akhova A.V.,Institute of Ecology and Genetics of Microorganisms |
Tkachenko A.G.,Institute of Ecology and Genetics of Microorganisms
FEMS Microbiology Letters | Year: 2014
The extensively discussed idea of oxidative stress development under antibiotic treatment was confirmed using an antioxidant gene expression (soxRS-, oxyR-regulon) approach, including microaerobic cultivation conditions. The killing action of antibiotics and their ability to cause peroxide oxidative stress in Escherichia coli cells was comparable to a similar hydrogen peroxide capacity; therefore, the involvement of intracellular hydrogen peroxide production in the killing action of antibiotics seems plausible under conditions studied. The temporary increase of ATP/ADP (which returned to untreated levels in 10 min) and the intensification of respiration preceded the development of oxidative stress. The sharp rise in ATP/ADP was due to the accumulation of ATP with a slight increase in the ADP content. We proposed that ATP accumulation was not a result of increased respiration but was due to the inhibition of energy-consuming processes. The association of reactive oxygen species formation under antibiotic treatment with the inhibition of direct electron flow pathway along the respiratory chain, and a possible role of a sharp rise in ATP/ADP in this process is hypothesized. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.