Entity

Time filter

Source Type


Podoprigora G.I.,Research Institute of Cytochemistry and Molecular Pharmacology
Vestnik Rossiiskoi Akademii Meditsinskikh Nauk | Year: 2013

Mononuclear-phagocyte system plays an important role in natural immunity and nonspecific resistance reactions of the organism against infection. The experimental studies using conventional laboratory animals with uncontrolled microflora have certain limitations to fully appreciate the role of autoflora (microbiota) in both development and functional activity of mononuclear- phagocyte system. In the present review of the author' systemic studies the value of microbiologically controlled animals (gnotobiotes) showing the multifaceted role of microbial factor on various maniflstations of mononuclear-phagocyte system including phagocytic activity of the cells of both ageing aspects and pathology conditions (inflammation, burns), influence on the colonization resistance and barrier function against translocations of microorganisms from the intestines, temperature reactions and other host defense mechanisms to infection are demonstrated. In a complex body defence, activity of both cellular and humoral factors is being stimulated and modulated by microbiotic factor. Gnotobiotic modeling approach is prospective one for modeling and analysis of molecular and cellular mechanisms in an assessment of the «host-microbiota» interactions, evaluating the effectiveness of new probiotic candidates and further microbial control development in the clinical settings. Source


Podoprigora G.I.,Research Institute of Cytochemistry and Molecular Pharmacology
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference | Year: 2012

Experimental studies using laboratory animal models have shown a potential vasoactive effect of natural metabolites such as glycine. The present study used intravital microscopy in laboratory rat models to study the microcirculation in the brain pial and mesentery vessels. To investigate the pial microvasculature, a stereotaxis-like animal fixing device was used. The intravital microscopy unit consisted of a binocular microscope equipped with a digital photo-video camera, processor, monitor and printer. Using reflected light, a special contact lens with an amplified focus depth provided high-resolution images of nontransparent tissue objects that typically have insufficient light exposure. Glycine had a vasodilatory effect on microvessels in the rat brain and mesenterium. The diameter of pial arterioles increased after glycine application especially markedly (up to 250% of initial size). These changes were not observed when physiological saline was used. Even a very small amount of glycine (a drop on the needle) was sufficient to stop the early stages of histamine-induced blood stasis development in 3-5 s in mesenterial microvessels. The vasodilatory effect of glycine on the pial microcirculation correlates with its reported positive therapeutic effect in cerebral ischemic stroke. The ability of glycine to avoid or prevent histamine-induced microcirculatory alterations in mesenterial microvessels may have potential clinical applications. Source


Barthod-Malat A.,University of Franche Comte | Kopylova V.,Research Institute of Cytochemistry and Molecular Pharmacology | Podoprigora G.I.,Research Institute of Cytochemistry and Molecular Pharmacology | Nartsissov Y.R.,Research Institute of Cytochemistry and Molecular Pharmacology | And 4 more authors.
Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS | Year: 2012

Computer simulation of biological systems for in silico validation has the potential of increasing the efficiency of pharmaceutical research and development by expanding the number of parameters tested virtually. Then only the most interesting subset of these has to be probed in vivo. By focusing on variables with the greatest influence on clinical end points, valuable drug targets can be advanced more quickly. A large number of methods have been developed to rebuild a three-dimensional (3D) model of a liver, mostly to prepare a liver surgery. These models are often not accurate and most of the them don't take into account the fluidics inside the vessels. © 2012 IEEE. Source


Podoprigora G.I.,Research Institute of Cytochemistry and Molecular Pharmacology | Blagosklonov O.,University of Franche Comte | Angoue O.,University of Franche Comte | Boulahdour H.,University of Franche Comte | Nartsissov Y.R.,Research Institute of Cytochemistry and Molecular Pharmacology
Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS | Year: 2012

Experimental studies using laboratory animal models have shown a potential vasoactive effect of natural metabolites such as glycine. The present study used intravital microscopy in laboratory rat models to study the microcirculation in the brain pial and mesentery vessels. © 2012 IEEE. Source


Lobysheva N.V.,Moscow State University | Selin A.A.,Research Institute of Cytochemistry and Molecular Pharmacology | Vangeli I.M.,Moscow State University | Byvshev I.M.,Moscow State University | And 2 more authors.
Free Radical Biology and Medicine | Year: 2013

Mitochondrial reactive oxygen species regulate many important biological processes. We studied H2O2 formation by nonsynaptic brain mitochondria in response to the addition of low concentrations of glutamate, an excitatory neurotransmitter. We demonstrated that glutamate at concentrations from 10 to 50 μM stimulated the H2O2 generation in mitochondria up to 4-fold, in a dose-dependent manner. The effect of glutamate was observed only in the presence of Ca2+ (20 μM) in the incubation medium, and the rate of calcium uptake by the brain mitochondria was increased by up to 50% by glutamate. Glutamate-dependent effects were sensitive to the NMDA receptor inhibitors MK-801 (10 μM) and D-AP5 (20 μM) and the inhibitory neurotransmitter glycine (5 mM). We have shown that the H 2O2 formation caused by glutamate is associated with complex II and is dependent on the mitochondrial potential. We have found that nonsynaptic brain mitochondria are a target of direct glutamate signaling, which can specifically activate H2O2 formation through mitochondrial respiratory chain complex II. The H2O2 formation induced by glutamate can be blocked by glycine, an inhibitory neurotransmitter that prevents the deleterious effects of glutamate in brain mitochondria. © 2013 Elsevier Inc. Source

Discover hidden collaborations