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Boratynski A.,Polish Academy of Sciences | Wachowiak W.,Polish Academy of Sciences | Dering M.,Polish Academy of Sciences | Boratynska K.,Polish Academy of Sciences | And 7 more authors.
Botanical Journal of the Linnean Society | Year: 2014

Despite Juniperus spp. being an important component of Mediterranean arid and semi-arid ecosystems, there is a lack of complex studies on their biogeographical patterns. Using 16 morphological cone and seed traits and three nuclear microsatellite markers, we investigated the morphological and genetic variability of seven Mediterranean and Macaronesian Juniperus taxa (J.oxycedrus ssp. oxycedrus, J.oxycedrus ssp. badia, J.brevifolia, J.cedrus, J.deltoides, J.macrocarpa and J.navicularis) to identify biogeographical trends and interspecific genetic relationships. The highest gene diversity was measured in J.oxycedrus ssp. oxycedrus (HE=0.716) and the lowest in J.brevifolia (HE=0.441). The west Mediterranean was characterized by a higher level of genetic diversity than the east Mediterranean. A lack of significant genetic differences between European and African populations of J.oxycedrus suggests that the Strait of Gibraltar was not a significant barrier to gene flow, but has promoted some morphological differentiation. The genetic and morphological results strongly support the recognition of J.macrocarpa, J.navicularis and J.deltoides at the species rank, whereas J.oxycedrus ssp. badia should be included in J.oxycedrus. © 2014 The Linnean Society of London.


Grant
Agency: European Commission | Branch: FP7 | Program: CPCSA | Phase: ICT-2013.1.8 | Award Amount: 6.17M | Year: 2014

CreatiFI addresses key challenges for ICT creative entrepreneurs in Europe in a decentralised way. CreatiFI integrates 4 Hubs located in Europes most creative Regions: Brussels, Barcelona, Helsinki, and Trento. CreatiFI hubs will provide business and technical guidance and assistance to regional SMEs and web entrepreneurs and beyond. CreatiFI offers (1) a very intensive dissemination and networking based on the Tier 1 European ICT and Creative Indystry Ecosystems; (2) Matchmaking between tech-savvy and creative people and organisations; (3) established and proven sets of procedures, methods, and tools; (4) technology and innovation support towards FI-PPP enablers; (6) Living Lab tests that enable SMEs to explore markets; and (7) Gateways to further funding.


Dzialuk A.,Kazimierz Wielki University in Bydgoszcz | Mazur M.,Kazimierz Wielki University in Bydgoszcz | Boratynska K.,Polish Academy of Sciences | Montserrat J.M.,Institute Of Cultura Of Barcelona | And 2 more authors.
Annals of Forest Science | Year: 2011

Introduction, Material and Methods The genetic structure and diversity of ten natural populations of Juniperus phoenicea L. from the western part of the species range have been studied using random amplified polymorphic DNA (RAPD) markers. Results and discussion Among 10 analyzed primers only 3 reproduced consistently across successful PCR reactions and gave 45 loci. The percentage of polymorphic loci (P) and Nei's heterozygosity (H e) have average values of 64.9% and 0.177. The average expected heterozygosity of particular populations positively correlate with latitude and negatively with altitude (τ=0.556, P=0.025; τ=?0.494, P=0.047, respectively). The proportion of genetic variation contributed by the differences between populations was low (GST=0.056). The gene flow (N m) has an average value of 4.2, and was higher in subsp. turbinata (7.3) than in subsp. phoenicea (4.1). Significant proportion of the variation (φST=0.106) was attributable to differences among populations, as revealed in analysis of molecular variance analysis of pair-wise RAPD distances. No evidence for isolation by distance was detected in Mantel test on genetic (φST) and geographic distances. European populations differed at a higher level from the African, subsp. phoenicea from turbinata (3.97% and 3.14% of total variance, respectively). The significant level of differences between European and African populations can result from (1) the earlier divergence and considerably low level of gene flow between them, or (2) a different mutation rate within population of different continent. Conclusion: The results suggest rather local forest economy with J. phoenicea, without seed exchange on large distance. © 2011 INRA and Springer Science+Business Media B.V.


Mazur M.,Kazimierz Wielki University in Bydgoszcz | Klajbor K.,Kazimierz Wielki University in Bydgoszcz | Kielich M.,Kazimierz Wielki University in Bydgoszcz | Sowinska M.,Kazimierz Wielki University in Bydgoszcz | And 3 more authors.
Dendrobiology | Year: 2010

The biometrical comparison of nine populations of Juniperus phoenicea from the western part of the species geographic range was the aim of the present study. Seven features of the cones and seeds, two of the shoots and leaves and eight proportions were studied biometrically and analysed using statistical methods. Two of analyzedpopulations, supposedto be representatives of J. phoenicea subsp. phoenicea, are closely related each other. The seven other populations representing J. phoenicea subsp. turbinata, appearedmuch more variable andd ifferedeach other at higher level. The results confirm the biochemical andgenetic differentiation of the species, however, it can partly be an effect of smaller number of comparedpopulations of subsp. phoenicea. The individuals included into each of distinguished subspecies are different and only a few individuals of subsp. turbinata from the Atlantic coast in Portugal were foundto be joinedinto the group of subsp. phoenicea. The differences between populations of subsp. turbinata are higher than between those of subsp. phoenicea. The most distant population of the subsp. turbinata, from Cabo Rizuto in Italy appears also the most different from all the other. It results probably from the isolation for a longer time.


Jasinska A.K.,Polish Academy of Sciences | Wachowiak W.,Polish Academy of Sciences | Muchewicz E.,Polish Academy of Sciences | Boratynska K.,Polish Academy of Sciences | And 2 more authors.
Botanical Journal of the Linnean Society | Year: 2010

We tested the performance of molecular markers and biometric traits in the identification of hybrids between closely related mountain pine (Pinus uncinata) and Scots pine (Pinus sylvestris). A plastid DNA marker and a set of morphological and anatomical needle traits were applied in analyses of individuals from several sympatric stands of the species and a single-species' population from southern Europe, used as a reference. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) marker from the plastid trnL- trnF region and morphological and anatomical traits clearly discriminated between the pure species. Significant differences were found between P. uncinata and P. sylvestris, mostly in the shape of epidermal cells and the number of stomata. Four putative hybrids with P. sylvestris morphology, but with P. uncinata plastid DNA haplotypes, were found in a population from Sierra de Gúdar near Valdelinares, the southernmost locality of the latter species in eastern Spain. Discrimination analyses between and within populations placed these individuals on the edge of an agglomeration of P. sylvestris individuals. The results suggest that hybridization between the species is rare, but can result in cryptic hybrids morphologically similar to the maternal species. © 2010 The Linnean Society of London.

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