Du X.M.,Shandong University |
Du X.M.,Laboratory Medicine |
Yan Y.,Institute of Crystal Materials |
Bai Z.L.,Shandong University |
And 4 more authors.
Biotechnic and Histochemistry | Year: 2010
Small organic dyes have been applied widely in fluorescence imaging techniques for biomedical research. We investigated the cytotoxicity of a novel fluorescent dye, trans-4-(N-2-hydroxyethyl-N-ethyl amino)-4′-(dimethyl amino) stilbene (DMAHAS), on human hepatocellular carcinoma (HepG2) cells using methyl thiazolyl tetrazolium(MTT), a neutral red assay, a Coomassie brilliant blue assay, and flow cytometric analysis. Our results showed that DMAHAS had live cell permeability, stable cytosolic localization and no significant cytotoxicity to HepG2 cells. We explored its application further for tumor cell tracking in a human liver tumor xenograft mouse model. Tumor xenografts were examined by fluorescence imaging and conventional histological methods. In addition, a method based on DMAHAS release was developed for tumor-specific cytotoxicity analysis. Our study indicated that DMAHAS is a reliable probe for tumor tracking and fluorescence imaging. © 2010 Biological Stain Commission.
Yu F.,Institute of Crystal Materials |
Zhang S.,Pennsylvania State University |
Zhao X.,Institute of Crystal Materials |
Yuan D.,Institute of Crystal Materials |
And 3 more authors.
Journal of Applied Physics | Year: 2011
The dielectric and electromechanical properties of fully ordered Ca 3TaGa3Si2O14 (CTGS) crystals were investigated over the temperature range of -60∼700 C. The highest electromechanical coupling factor, k26 (18.9%) and piezoelectric coefficient, d26 (-11.5 pC/N) were obtained for (YXl)-25° cuts. The temperature dependent behavior of resonance frequency (fr) was investigated in single-rotated thickness shear mode (TSM) (YXl)θ cuts (θ-35°∼10°). The turnover temperatures of resonance frequency were found to increase from 20°C to 330°C, as the rotation angle varied from -22.5° to -35°. Bulk acoustic wave (BAW) resonators based on Y(-30) monolithic disks with a fundamental frequency ∼2.87 MHz were fabricated, where the in air mechanical quality factor Q was found to be on the order of 24000 and 10000 at 20°C and 700°C, respectively. The high coupling k26, high mechanical Q, and high electrical resistivity (16 Mcm) at 700°C, together with the near zero TCF characteristics at elevated temperatures, demonstrate the potential of CTGS crystals for high temperature sensor applications. © 2011 American Institute of Physics.
Sun G.H.,Institute of Crystal Materials |
Zhang G.H.,Institute of Crystal Materials |
Wang X.Q.,Institute of Crystal Materials |
Sun Z.H.,Institute of Crystal Materials |
Xu D.,Institute of Crystal Materials
Materials Chemistry and Physics | Year: 2010
The nucleation parameters such as critical radius, volume free energy change and critical free energy barrier have been evaluated for deuterated l-arginine trifluoroacetate crystals on the basis of the classical homogeneous nucleation theory. Solution stability was studied by observing the metastable zone width employing a polythermal method and it revealed that the deuterated solution has a larger metastable zone width than its non-deuterated solution. Induction periods for different saturated solutions were also determined based on isothermal method. A good quality crystal has been successfully grown by optimizing its nucleation parameters, and its etching studies illustrated that the crystal grows in a two-dimensional nucleation mechanism. © 2010 Elsevier B.V. All rights reserved.