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Krasilnikova A.A.,Novosibirsk State University | Shestopalov M.A.,RAS Nikolaev Institute of Inorganic Chemistry | Brylev K.A.,RAS Nikolaev Institute of Inorganic Chemistry | Kirilova I.A.,Novosibirsk Research Institute Of Traumatology And Orthopaedics Na Yal Tsivyan | And 5 more authors.
Journal of Inorganic Biochemistry | Year: 2015

Investigation of new X-ray contrast media for radiography is an important field of science since discovering of X-rays in 1895. Despite the wide diversity of available X-ray contrast media the toxicity, especially nephrotoxicity, is still a big problem to be solved. The octahedral metal-cluster complexes of the general formula [{M6Q8}L6] can be considered as quite promising candidates for the role of new radiocontrast media due to the high local concentration of heavy elements, high tuning ability of ligand environment and low toxicity. To exemplify this, the X-ray computed tomography experiments for the first time were carried out on some octahedral cluster complexes of molybdenum and rhenium. Based on the obtained data it was proposed to investigate the toxicological proprieties of cluster complex Na2H8[{Re6Se8}(P(CH2CH2CONH2)(CH2CH2COO)2)6]. Observed low cytotoxic and acute toxic effects along with rapid renal excretion of the cluster complex evidence its perspective as an X-ray contrast media for radiography. © 2014 Elsevier Inc.

PubMed | The Institute of Molecular Biology and Biophysics, Novosibirsk State University, Scientific Institute of Clinical and Experimental Lymphology and RAS Nikolaev Institute of Inorganic Chemistry
Type: | Journal: Journal of inorganic biochemistry | Year: 2016

Inclusion compounds of photoluminescent hexamolybdenum cluster complexes in the chromium terephthalate metal-organic framework, MIL-101 (MIL, Matrial Institut Lavoisier) were successfully synthesized in two different ways and characterized by means of powder X-Ray diffraction, chemical analysis and nitrogen sorption. Some important functional properties of hexamolybdenum cluster complexes for biological and medical applications, in particular singlet oxygen generation ability, luminescence properties, cellular uptake behavior and cytotoxicity were studied. It was revealed that the inclusion compounds possessed significant singlet oxygen generation activity. The materials obtained showed a low cytotoxicity, thus allowing them to be used in living cells. Confocal microscopy of human larynx carcinoma (Hep-2) cells incubated with the inclusion compounds showed that MIL-101 performed as a nanocarrier adhering to the external cell membrane surface and releasing the cluster complexes which that penetrated into the cells. Moreover, photoinduced generation of reactive oxygen species (ROS) in Hep-2 cells incubated with inclusion compounds was demonstrated. The cluster supported on MIL-101 was shown to possess in vivo phototoxicity.

PubMed | Research Institute of Experimental and Clinical Medicine, Novosibirsk State University, Scientific Institute of Clinical and Experimental Lymphology, RAS Nikolaev Institute of Inorganic Chemistry and Meshalkin Novosibirsk Research Institute of Blood Circulation Pathology
Type: | Journal: Nanomedicine : nanotechnology, biology, and medicine | Year: 2016

Octahedral rhenium cluster complexes may have considerable potential as therapeutic and diagnostic drugs due to their luminescent and X-ray contrast properties, as well as their ability to generate singlet oxygen upon photoirradiation. However, their potential biological effects and toxicity in vitro and in vivo are rather far from being understood. Thus, the aim of our research was to study cytotoxicity, intracellular localization and cellular uptake/elimination kinetics in vitro, biodistribution and acute intravenous toxicity in vivo of a complex Na

PubMed | University Canada West, Scientific Institute of Clinical and Experimental Lymphology, University of Missouri - Kansas City, Scientific Research Institute of Physiology and Basic Medicine and 3 more.
Type: Journal Article | Journal: The Journal of pharmacy and pharmacology | Year: 2016

We evaluated the hypolipidaemic effect of mannan Candida albicans serotype A, relative to atorvastatin, in a mouse model of hyperlipidaemia.Mannan serotype A was investigated in vitro and in vivo to determine its effects on macrophage proliferation, nitric oxide (NO) production by cultured macrophages, serum and liver lipids, changes in liver morphology and serum chitotriosidase activity and its expression in the liver.Mannan serotype A stimulates the macrophage proliferation and NO production in murine peritoneal macrophages in vitro. The activity of serum chitotriosidase (an enzyme released from the activated macrophages) was found to be significantly increased in P-407-induced hyperlipidaemic mice pretreated with low-dose mannan compared with mice administered P-407 only. Mannan treatment in mice was shown to significantly increase the chitotriosidase expression in the liver of both non-hyperlipidaemic and P-407-induced hyperlipidaemic mice. Lastly, mice pretreated with mannan before the induction of hyperlipidaemia with P-407 showed a significant reduction in the serum concentration of atherogenic LDL cholesterol, total cholesterol, triglycerides and liver triglycerides.It is suggested that mannan serotype A, like -glucan, may represent another hypolipidaemic agent, which could potentially be used as an adjunctive therapy with conventional antihyperlipidaemic drugs (statins and fibrates) in humans.

Shevchenko A.V.,Scientific Institute of Clinical and Experimental Lymphology | Konenkov V.I.,Scientific Institute of Clinical and Experimental Lymphology | Prokofev V.F.,Scientific Institute of Clinical and Experimental Lymphology | Ragino Yu.I.,Institute of Internal and Preventive Medicine | And 2 more authors.
Kardiologiya | Year: 2016

Great number of factors stimulating or inhibiting production of proteins in inflammatory process influence serum levels of markers of inflammation. A number of homozygous genotypes of inflammation, destruction, and angiogenesis genes have been found to be associated with basic clinical-laboratory indices of inflammation and atherosclerotic process. The revealed genetic markers can be used as complimentary markers of prognosis of the disease course.

Klimontov V.V.,Scientific Institute of Clinical and Experimental Lymphology | Myakina N.E.,Scientific Institute of Clinical and Experimental Lymphology
Diabetes Mellitus | Year: 2015

Aim. To assess the relationship of glucose variability (GV) and renal function in patients with type 2 diabetes on basal-bolus insulin therapy. Materials and methods. We observed 101 females with type 2 diabetes, aged 47-79 years, with a glomerular filtration rate (GFR) >30 mL/min/1.73 m2. Insulin was combined with metformin in 45 of these women. The mean glucose and standard deviation, continuous overlapping net glucose action, lability index, J-index, low blood glucose index (LBGI), high blood glucose index (HBGI), M-value and mean absolute glucose (MAG) were calculated based on the results of blinded continuous glucose monitoring. The prevalence of episodes of low interstitial glucose (<3.9 and 2.8 mmol/L) of at least 20-min duration was estimated. Results. Patients with a GFR of 30-44 mL/min/1.73 m2 had significantly lower HBGI, J-index, MAG and M-value compared with those with better filtration (all p < 0.05); LBGI was not dependent on GFR. The GFR values were weakly and positively correlated with HBGI, J-index, M-value and MAG. Multiple regression analysis showed that GFR is an independent predictor of MAG (p = 0.04). No significant differences were found in the prevalence of episodes of low interstitial glucose between patients with different GFR ranges. Conclusions. GV parameters are related to renal function in type 2 diabetic women on basal-bolus insulin therapy. Patients with stage 3b chronic kidney disease have reduced GV, predominantly in the hyperglycaemic band, compared with those with better filtration.

Klimontov V.V.,Scientific Institute of Clinical and Experimental Lymphology | Fazullina O.N.,Scientific Institute of Clinical and Experimental Lymphology
Diabetes Mellitus | Year: 2015

Aim: To determine the relationship between bone mineral density (BMD) and total body composition in postmenopausal women with type 2 diabetes. Materials and Methods: The study included 78 women, from 50 to 70 years of age (median 63 years). Twenty women had normal body mass index (BMI), 29 ones were overweight and 29 had obesity. The body composition and BMD was studied by dual-energy X-ray absorptiometry. Results: Women with normal BMD had higher BMI, total and truncal fat mass, as well lean mass as compared to women with osteoporosis and osteopenia (all p <0.05). Patients with osteoporosis had a lower fat mass at the hips, compared with those with normal BMD. Total and truncal fat mass, as well as lean mass were positively correlated with BMD in the lumbar spine and proximal femur, femoral neck and radius. In multivariate regression analysis fat mass was an independent predictor for total BMD, after adjusting for age, BMI, duration of menopause, HbA1c, glomerular filtration rate and other total body composition parameters. Conclusions: In postmenopausal type 2 diabetic women BMI and fat mass is associated positively with BMD. © 2015.

Korbut A.I.,Scientific Institute of Clinical and Experimental Lymphology | Klimontov V.V.,Scientific Institute of Clinical and Experimental Lymphology
Diabetes Mellitus | Year: 2016

Glucagon like peptide-1 (GLP-1) analogues and dipeptidyl peptidase-4 (DPP-4) inhibitors are new classes of hypoglycemic agents with numerous pleiotropic effects. The review summarises data about the influence of GLP-1 analogues and DPP-4 inhibitors on structural and functional changes in diabetic kidneys. Growing evidence indicates that the kidney is one of the loci of the effects and degradation of GLP-1. The potency of the effects of GLP-1 in diabetic kidneys can be reduced by decrease in GLP-1 receptor expression or enhancement of GLP-1 degradation. In experimental models of diabetic nephropathy and non-diabetic renal injury, GLP-1 analogues and DPP-4 inhibitors slow the development of kidney fibrosis and prevent the decline of kidney function. The mechanisms of protective effect include hyperglycaemia reduction, enhancement of sodium excretion, suppression of inflammatory and fibrogenic signalling pathways, reduction of oxidative stress and apoptosis in the kidneys. In clinical studies, the urinary albumin excretion reduction rate while using the GLP-1 analogue and DPP-4 inhibitor treatment was demonstrated in patients with type 2 diabetes. Long-term impact of these agents on renal function in diabetes needs further investigations.

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