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Kello M.,Pavol Jozef Safarik University | Drutovic D.,Pavol Jozef Safarik University | Chripkova M.,Pavol Jozef Safarik University | Pilatova M.,Pavol Jozef Safarik University | And 5 more authors.
Molecules | Year: 2014

This study was designed to examine the in vitro antiproliferative effect of brassinin and its derivatives on human cancer cell lines. Among seven tested compounds, homobrassinin (K1; N-[2-(indol-3-yl)ethyl]-S-methyldithiocarbamate) exhibited the most potent activity with IC50 = 8.0 μM in human colorectal Caco2 cells and was selected for further studies. The flow cytometric analysis revealed a K1-induced increase in the G2/M phase associated with dysregulation of α-tubulin, α1-tubulin and β5-tubulin expression. These findings suggest that the inhibitory effect of K1 can be mediated via inhibition of microtubule formation. Furthermore, simultaneously with G2/M arrest, K1 also increased population of cells with sub-G1 DNA content which is considered to be a marker of apoptotic cell death. Apoptosis was also confirmed by annexin V/PI double staining, DNA fragmentation assay and chromatin condensation assay. The apoptosis was associated with the loss of mitochondrial membrane potential (MMP), caspase-3 activation as well as intracellular reactive oxygen species (ROS) production. Moreover, the antioxidant Trolox blocked ROS production, changes in MMP and decreased K1 cytotoxicity, which confirmed the important role of ROS in cell apoptosis. Taken together, our data demonstrate that K1 induces ROS-dependent apoptosis in Caco2 cells and provide the rationale for further in vivo anticancer investigation. © 2014 by the authors. Source

Anjum S.,CAS Changchun Institute of Applied Chemistry | Anjum S.,University of Chinese Academy of Sciences | Anjum S.,Islamia University of Bahawalpur | Anjum S.,Women University | And 10 more authors.
Journal of Electroanalytical Chemistry | Year: 2015

The in situ electrogenerated aryl diazonium salts obtained by the electrochemical reduction of nitro precursor, p-nitrophenyl aldehyde, in the presence of nitrous acid was used for the modification of glassy carbon electrodes. The as-prepared modified electrode shows good electrocatalytical activity toward the reduction of hydrogen peroxide, and was employed for the construction of hydrogen peroxide sensor. The hydrogen peroxide sensor has high sensitivity, low detection limit and wide linear range. The sensor also exhibited excellent stability and anti-interference capability for the detection of hydrogen peroxide. It has been successfully applied to the determination of hydrogen peroxide in real samples. © 2015 Elsevier B.V. All rights reserved. Source

Ayaz S.,Institute of Chemical science | Ishaq M.,Institute of Chemical science | Ahmed I.,Institute of Chemical science | Khalil N.K.,Institute of Chemical science
Journal of Vinyl and Additive Technology | Year: 2015

The preparation of binary polymer blend nanocomposites with different nanomaterials is a relatively new approach to achieve desired physical, thermal, mechanical, and electrochemical properties because it has the collective effects of both polymer blending and fillers. Transition metal oxides constitute a large class among those fillers because the precursors for metal oxides are abundantly available. However, very few studies have been accomplished on incorporating transition metal oxides into binary polymer blends. In this project, cuprous oxide (CuO) nanoparticles (NPs) with a crystallite size of 24.95 nm were incorporated into poly(vinyl chloride)/poly(methyl methacrylate) (PVC/PMMA) blend, and thin films of the nanocomposites were obtained through a solution casting technique. Scanning electron microscopy, X-ray diffraction, universal testing machine testing, thermogravimetric analysis, and cyclic voltammetry were used to study morphological, crystalline, mechanical, thermal, and electrochemical properties of the nanocomposites. Scanning electron micrographs showed that the blend was completely miscible and CuO NPs were well dispersed within the matrix. Mechanical properties greatly improved with each wt% addition of CuO NPs. Thermogravimetric analysis thermograms revealed a two-stage degradation for neat PVC/PMMA blend and CuO/PVC/PMMA. Cyclic voltammetry results indicated a free electron transfer in neat blend that further improved with the incorporation of increasing percentage of CuO NPs. © 2015 Society of Plastics Engineers. Source

Ahmad A.,State Key Laboratory of Chemical Resource Engineering | Syed F.,Institute of Chemical science | Imran M.,Institute of Chemical science | Khan A.U.,State Key Laboratory of Chemical Resource Engineering | And 3 more authors.
Journal of Food Biochemistry | Year: 2015

In this report, we present a simple and green approach to synthesize gold nanoparticles (AuNPs) with efficient antileishmanial activity. Phytochemicals from the aqueous extract Maytenus royleanus stem were used to reduce and stabilize the gold ions into metallic nanoparticles. UV-Vis spectroscopy was employed to detect the characteristic surface plasmon resonance pattern of AuNPs (550 nm). X-ray diffraction (XRD) and energy dispersive X-ray studies were carried out to determine the crystalline nature and elemental composition of AuNPs. The surface functionalities of nanoparticles were identified with Fourier-transform infrared spectroscopy. XRD and transmission electron microscopy revealed that AuNPs were mostly hexagonal in shape with an approximate particle size of 30 nm. Significant antileishmanial activity was shown by AuNPs. AuNPs inhibited the parasite growth by 75% after 72 h of incubation. The findings of this study indicate that these nanoparticles could be an alternative, safe, and effective source of antileishmanial agents. © 2015 Wiley Periodicals, Inc. Source

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