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Radulescu M.,Polytechnic University of Bucharest | Andronescu E.,Polytechnic University of Bucharest | Holban A.M.,Polytechnic University of Bucharest | Holban A.M.,University of Bucharest | And 7 more authors.
Metals | Year: 2016

The aim of this study was to develop a biocompatible coating for wound dressings, containing iron oxide nanoparticles functionalized with patchouli essential oil in order to obtain improved antimicrobial properties able to prevent biofilm development and consecutive associated infections. The bioactive coating was prepared by the co-precipitation of a precursor in an alkaline solution of patchouli oil. The prepared surface was characterized by XRD (X ray diffraction), TEM (transmission electron microscopy), SAED (selected area diffraction), SEM (scanning electron microscopy) and FT-IR (Fourier transform infrared spectroscopy). The bioevaluation of the obtained coating consisted in antimicrobial, as well as in vitro and in vivo biocompatibility and biodistribution assays. The obtained coating revealed a strong anti-biofilm activity maintained up to 72 h, as well as a low cytotoxicity on mammalian cells and a good biodistribution after intraperitoneal injection in mice. These results demonstrate the promising potential of the respective coatings for the management of wound infections and for the development of soft materials with improved resistance to microbial colonization. © 2016 by the authors; licensee MDPI, Basel, Switzerland.


PubMed | Institute of Cellular Biology and Pathology of Romanian Academy, Stefan S Nicolau Institute Of Virology, Polytechnic University of Bucharest, University of Medicine and Pharmacy of Craiova and University of Bucharest
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2014

This paper reports the synthesis and characterization of amoxicillin- functionalized magnetite nanostructures (Fe3O4@AMO), revealing and discussing several biomedical applications of these nanomaterials. Our results proved that 10 nm Fe3O4@AMO nanoparticles does not alter the normal cell cycle progression of cultured diploid cells, and an in vivo murine model confirms that the nanostructures disperse through the host body and tend to localize in particular sites and organs. The nanoparticles were found clustered especially in the lungs, kidneys and spleen, next to the blood vessels at this level, while being totally absent in the brain and liver, suggesting that they are circulated through the blood flow and have low toxicity. Fe3O4@AMO has the ability to be easily circulated through the body and optimizations may be done so these nanostructures cluster to a specific target region. Functionalized magnetite nanostructures proved a great antimicrobial effect, being active against both the Gram positive pathogen S. aureus and the Gram negative pathogen E. coli. The fabricated nanostructures significantly reduced the minimum inhibitory concentration (MIC) of the active drug. This result has a great practical relevance, since the functionalized nanostructures may be used for decreasing the therapeutic doses which usually manifest great severe side effects, when administrated in high doses. Fe3O4@AMO represents also a suitable approach for the development of new alternative strategies for improving the activity of therapeutic agents by targeted delivery and controlled release.


Grumezescu V.,Romanian National Institute for Lasers, Plasma and Radiation Physics | Grumezescu V.,Polytechnic University of Bucharest | Holban A.M.,University of Bucharest | Iordache F.,Institute of Cellular Biology and Pathology of Romanian Academy | And 8 more authors.
Applied Surface Science | Year: 2014

This study reports the biological applications of a newly fabricated water dispersible nanostructure, based on magnetite (Fe3O4) and eugenol (E), prepared in a well-shaped spherical form by precipitation method. The presence of Fe3O4@E nanoparticles has been confirmed by transmission electron microscopy (TEM). Nanoparticles have been embedded into poly(3-hidroxybutyric acid-co-3-hidroxyvaleric acid)-polyvinyl alcohol (P(3HB-3HV)-PVA) microspheres by oil-in-water emulsion technique. Functionalized P(3HB-3HV)-PVA-Fe3O4@E microspheres coatings have been fabricated by matrix assisted pulsed laser evaporation (MAPLE). The coatings have been characterized by infrared microscopy (IRM) and scanning electron microscopy (SEM). In vitro biofilm formation by Staphylococcus aureus and Pseudomonas aeruginosa was assessed by the viable cell counts technique. Nanomaterial biocompatibility has been investigated by analyzing the phenotypic changes of cultured eukaryotic cells. Besides their excellent anti-adherence and anti-biofilm properties, the MAPLE coatings have the advantages of using bioactive natural compounds, which are less toxic and easily biodegradable than current antibiotics. This approach could be used as a successful alternative or adjuvant method to control and prevent microbial biofilms associated infections. © 2014 Elsevier B.V. All rights reserved.


Grumezescu V.,Polytechnic University of Bucharest | Grumezescu V.,Romanian National Institute for Lasers, Plasma and Radiation Physics | Andronescu E.,Romanian National Institute for Lasers, Plasma and Radiation Physics | Holban A.M.,Polytechnic University of Bucharest | And 8 more authors.
Applied Surface Science | Year: 2015

The purpose of this study was the fabrication of functionalized anti-adherent surfaces based on the polyvinyl chloride (PVC) coated with 3-amino propyltrimethoxysilane (APTMS) by matrix assisted pulsed laser evaporation (MAPLE) in order to improve the resistance of PVC based prosthetic devices to microbial colonization. Infrared microscopy (IRM) investigations of APTMS thin films proved the compositional homogeneity of the prepared thin film. Scanning electron microscopy (SEM) micrographs revealed a granular morphology with microspheres harboring a diameter between 15 and 60 nm. The microbiological assays proved that MAPLE deposited APTMS films inhibited the adherence capacity and biofilm development of Pseudomonas aeruginosa and Staphylococcus aureus strains. Furthermore, this material proved to be highly biocompatible, allowing the normal growth and development of human endothelial cells. These traits highlight the fact that the fabricated APTMS thin films may be efficiently used for improving different surfaces of medical use, including prostheses and implantable devices. © 2015 Elsevier B.V. All rights reserved.


Iordache F.,Institute of Cellular Biology and Pathology of Romanian Academy | Oprea A.E.,Polytechnic University of Bucharest | Grumezescu V.,Polytechnic University of Bucharest | Grumezescu V.,Romanian National Institute for Lasers, Plasma and Radiation Physics | And 8 more authors.
Journal of Sol-Gel Science and Technology | Year: 2015

Abstract: Poly(lactic-co-glycolic) acid/chitosan microsphere coatings containing Cinnamomi aetheroleum-functionalized magnetite nanoparticles (Fe3O4@CA) were deposited by matrix-assisted pulsed laser evaporation in order to improve the surface resistance to microbial colonization. The obtained surface proved a very good biocompatibility and demonstrates that bacterial colonization is impaired on the nanomodified bioactive surfaces and also Staphylococcus aureus biofilm formation is significantly altered. © 2015, Springer Science+Business Media New York.


Grumezescu A.M.,Polytechnic University of Bucharest | Andronescu E.,Polytechnic University of Bucharest | Oprea A.E.,Polytechnic University of Bucharest | Holban A.M.,Polytechnic University of Bucharest | And 7 more authors.
Journal of Sol-Gel Science and Technology | Year: 2015

Abstract: This paper reports a new bioactive surface based on magnetic microspheres with an average size up to 500 nm. Magnetic microspheres consisting of 8 nm magnetite nanoparticles functionalized with Melissa officinalis essential oil, poly lactic acid and chitosan were prepared using a solvent evaporation method and pelliculized by MAPLE. The as prepared surface was characterized by X-ray diffraction, infrared and scanning electron microscopy. The in vitro experiments revealed a good compatibility with eukaryotic cells a significant inhibitory activity of the prokaryotic cells adherence properties. This approach could be a successful method to control and prevent microbial biofilms associated infections and may be also used as an alternative strategy to limit the consumption of synthetic antimicrobial drugs in order to decrease the rates of microbial resistance to antibiotics. © 2014, Springer Science+Business Media New York.


Holban A.M.,Polytechnic University of Bucharest | Holban A.M.,University of Bucharest | Andronescu E.,Polytechnic University of Bucharest | Grumezescu V.,Polytechnic University of Bucharest | And 7 more authors.
Journal of Sol-Gel Science and Technology | Year: 2015

In this study, we aimed to improve and extend the microbiological applications of magnetic nanocarriers, by the successful fabrication of a new nanostructured surface based on 7 nm average diameter magnetite and carvone (Fe3O4@CAR) nanoparticles and to evaluate the biological activity of this material deposed as a thin film by advanced laser techniques (MAPLE). The results demonstrate that the thin surfaces containing the fabricated bio-active nanosystem have a great antimicrobial activity, inhibiting the colonization and biofilm formation of both Gram positive and Gram negative tested species. Furthermore, the in vitro results demonstrate that this material is not cytotoxic, allowing the normal growth and development of human endothelial cells. Our results demonstrate that the fabricated magnetic bioactive nanostructured surfaces have a great potential to be used for the development of novel applications on the medical field, particularly in the control of infectious diseases. © 2014, Springer Science+Business Media New York.


PubMed | Institute of Cellular Biology and Pathology of Romanian Academy, Romanian National Institute for Research and Development in for Textile and Leather, Polytechnic University of Bucharest and University of Bucharest
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2016

The aim of this study was to develop, characterize and assess the biological activity of a new regenerative 3D matrix with antimicrobial properties, based on collagen (COLL), hydroxyapatite (HAp), -cyclodextrin (-CD) and usnic acid (UA). The prepared 3D matrix was characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Microscopy (FT-IRM), Transmission Electron Microscopy (TEM), and X-ray Diffraction (XRD). In vitro qualitative and quantitative analyses performed on cultured diploid cells demonstrated that the 3D matrix is biocompatible, allowing the normal development and growth of MG-63 osteoblast-like cells and exhibited an antimicrobial effect, especially on the Staphylococcus aureus strain, explained by the particular higher inhibitory activity of usnic acid (UA) against Gram positive bacterial strains. Our data strongly recommend the obtained 3D matrix to be used as a successful alternative for the fabrication of three dimensional (3D) anti-infective regeneration matrix for bone tissue engineering.


Zanfir A.V.,Polytechnic University of Bucharest | Voicu G.,Polytechnic University of Bucharest | Busuioc C.,Polytechnic University of Bucharest | Jinga S.I.,Polytechnic University of Bucharest | And 2 more authors.
Materials Science and Engineering C | Year: 2016

The integration of ceramic powders in composite materials for bone scaffolds can improve the osseointegration process. This work was aimed to the synthesis and characterization of new collagen-hydroxyapatite/barium titanate (Coll-HA/BT) composite materials starting from barium titanate (BT) nanopowder, hydroxyapatite (HA) nanopowder and collagen (Coll) gel. BT nanopowder was produced by combining two wet-chemical approaches, sol-gel and hydrothermal methods. The resulting materials were characterized in terms of phase composition and microstructure by X-ray diffraction, Raman spectroscopy, scanning electron microscopy and transmission electron microscopy. Moreover, the biocompatibility and bioactivity of the composite materials were assessed by in vitro tests. The synthesized BT particles exhibit an average size of around 35 nm and a spherical morphology, with a pseudo-cubic or tetragonal symmetry. The diffraction spectra of Coll-HA and Coll-HA/BT composite materials indicate a pronounced interaction between Col and the mineral phases, meaning a good mineralization of Col fibres. As well, the in vitro tests highlight excellent osteoinductive properties for all biological samples, especially for Coll-HA/BT composite materials, fact that can be attributed to the ferromagnetic properties of BT. © 2016 Elsevier B.V. All rights reserved.


PubMed | Institute of Cellular Biology and Pathology of Romanian Academy and Polytechnic University of Bucharest
Type: Journal Article | Journal: International journal of pharmaceutics | Year: 2016

This work presents a study based on the preparation and characterization of drug-collagen hybrid materials. Materials used for obtaining drug-collagen hybrids were collagen type I (Coll) as matrix and fludarabine (F) and epirubicin (E) as hydrophilic active substances. After incorporation of drugs into Coll in different ratios, the obtained hybrid materials (Coll/F and Coll/E) could be used according to our results as potential drug delivery systems in medicine for the topical (local) treatment of cancerous tissues (e.g. the treatment of breast, stomach, lung, colorectal or advanced ovarian cancer). The materials were characterized considering their composition (by XRD, FT-IR and DTA-TG) and their morphology (by SEM). The delivery of drug was assessed by UV-vis. The in vitro citotoxicity demonstrates an antitumoral activity of the obtained hybrid materials and their potential use for biomedical applications as drug delivery systems in tumoral treatments.

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