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Shablii V.A.,NASU Institute of Molecular Biology and Genetics | Shablii V.A.,Institute of Cell Therapy | Kuchma M.D.,Institute of Cell Therapy | Kyryk V.M.,Ukrainian Academy of Sciences | Lobyntseva G.S.,Institute of Cell Therapy
Cytology and Genetics | Year: 2012

The presence of a viable population of hepatoblasts, epithelial blast cells, and endothelial and mesenchymal cells in a cryoconserved suspension of human fetal liver (FL) cells was shown. Epithelial-mesenchymal hepatoblast transformation in a cell culture was found. A possibility to apply the previously developed method of cryoconservation of human FL hematopoietic cells in the first trimester of gestation for the preservation of the heterogenic population of parenchymal and stromal cells was shown. © Allerton Press, Inc., 2012. Source


Shablii V.A.,NASU Institute of Molecular Biology and Genetics | Shablii V.A.,Institute of Cell Therapy | Lukash L.L.,NASU Institute of Molecular Biology and Genetics | Lobintseva G.S.,Institute of Cell Therapy
Cytology and Genetics | Year: 2012

This review of publications is dedicated to the analysis of experimental data concerning the pos-sible mechanisms constituting the basis of transdifferentiation or plasticity of tissue-specific stem cells. In the review, we focused on the mechanisms and genetic consequences of fusion between donor cells and recipient tissue cells that were investigated using models of cell therapy for liver and heart diseases. The role of inter-cellular contacts of different types and horizontal gene transfer during the heart tissue regeneration process was also considered. © 2012 Allerton Press, Inc. Source


Shablii V.A.,NASU Institute of Molecular Biology and Genetics | Shablii V.A.,Institute of Cell Therapy | Kuchma M.D.,NASU Institute of Molecular Biology and Genetics | Kuchma M.D.,Institute of Cell Therapy | And 5 more authors.
Biopolymers and Cell | Year: 2014

Aim. The investigation of trophoblast markers expression in the mesenchymal stromal cells of placental tissue before and after their isolation and cultivation. Methods. Placental multipotent mesenchymal stromal cells (MMSC) were obtained by culturing the adhesive fraction of cells enzymatically isolated from the placental tissue. The placental derived cells were immunophenotyped by flow cytometry and immunocytoche-mistry. Results. It was shown that the placental MMSC express the trophoblast markers: human chorionic gonadotropin, pan-cytokeratin, epidermal growth factor receptor HER2. MMSC-like populations of CD90+ CD73+CD45-CD34-CD14--cells and CD90-CD73+CD45-CD34-CD14--cells are presented in the placental tissue. Conclusions. The placental MMSC simultaneously express mesenchymal and trophoblast markers. © Institute of Molecular Biology and Genetics, NAS of Ukraine, 2014. Source


Kuchma M.D.,Institute of Cell Therapy | Kuchma M.D.,NASU Institute of Molecular Biology and Genetics | Kyryk V.M.,Ukrainian Academy of Sciences | Svitina H.M.,Institute of Cell Therapy | And 4 more authors.
BioMed Research International | Year: 2015

We have investigated the characteristics of human hematopoietic progenitor cells (HPCs) with the CD34+CD45lowSSClow phenotype from full-term placental tissue (FTPT) as compared to cord blood (CB) and fetal liver (FL) cells. We demonstrated the presence of cell subpopulations at various stages of the differentiation with such immunophenotypes as CD34+/lowCD45low/-, CD34++CD45low/-, CD34+++CD45low/-, CD34+/lowCD45hi, and CD34++CD45hi in both first trimester placental tissue (FiTPT) and FTPT which implies their higher phenotypic heterogeneity compared to CB. HPCs of the FTPT origin expressed the CD90 antigen at a higher level compared to its expression by the CB HPCs and the CD133 antigen expression being at the same level in both cases. The HPCs compartment of FTPT versus CB contained higher number of myeloid and erythroid committed cells but lower number of myeloid and lymphoid ones compared to FL HPCs. HPCs of the FTPT and CB origin possess similar potentials for the multilineage differentiation in vitro and similar ratios of myeloid and erythroid progenitors among the committed cells. This observation suggests that the active hematopoiesis occurs in the FTPT. We obtained viable HPCs from cryopreserved placental tissue fragments allowing us to develop procedures for banking and testing of placenta-derived HPCs for clinical use. © 2015 Maria D. Kuchma et al. Source


Shablii V.A.,NASU Institute of Molecular Biology and Genetics | Shablii V.A.,Institute of Cell Therapy | Kuchma M.D.,NASU Institute of Molecular Biology and Genetics | Kuchma M.D.,Institute of Cell Therapy | And 4 more authors.
Cellular Transplantation and Tissue Engineering | Year: 2012

In this paper we have show the possibility of cryopreservation of placental tissue for the reason to obtain viable hematopoietic progenitor cells and multipotent mesenchymal stromal cells. It is established, that the relative content of the cells of CD34+/ SSClow/CD45low cells significantly higher in the cryopreserved placental tissue. Also we first described the differences expression of CD90 and CD31on the CD34+/CD45low/SSClow cells of umbilical cord blood and placenta. Clonal analysis of cells revealed the presence in cryopreserved placental tissues of precursors of granulocytes, monocytes and erythrocytes that forming colonies of granulocyte-monocyte, monocytes, granulocytes and erythrocytes. Also it was obtained cultures of stromal cells from cryopreserved placental tissues with immunophenotype CD90+/CD73+/CD105+/ HLA-ABClow/CD45-/ CD34-/CD133-/CD14- and adipogenic and osteogenic potential in vitro. Source

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