Wang D.,Jiangsu University |
Zhou J.,Institute of Cancer Research of Jiangsu Province |
Wang T.,Institute of Cancer Research of Jiangsu Province |
Li X.,Jiangsu University |
And 5 more authors.
Background: Although polymorphisms in DNA mismatch repair (MMR) gene MSH2 have been associated with risks of many cancers, little is known about their etiology role in gastric cancer (GC) and the potential interacting role with lifestyle factors known to damage DNA. Methods: A population-based study was conducted in 3 counties (Jintan, Taixing and Huaian) of Jiangsu Province, the high-risk areas of GC in China. We investigated the association of polymorphisms IVS12-6T> C and IVS10+12G> A in MSH2 gene with the risk of GC and the potential gene-lifestyle interaction. Results: The risk of GC was found to be associated with the IVS12-6C allele (CC vs TT, OR = 2.34, 95% CI: 1.17-4.71) and IVS10+12A allele (GA or AA vs GG, OR = 1.55, 95% CI: 1.14-2.21; and GA vs GG, OR = 1.51, 95% CI: 1.04-2.17). Stratified analysis indicated that an increased risk of GC also was observed in: suspected familial subjects carrying the IVS12-6T> C (OR = 1.68, 95% CI: 1.27-2.66) or IVS10+12G> A (OR = 2.57, 95% CI: 1.53-4.10); or younger subjects carrying the IVS12-6T> C (OR = 2.15, 95% CI: 1.24-3.91) or IVS10+12G> A (OR = 2.23, 95% CI: 1.20-4.33); or male subjects carrying the IVS10+12G> A (OR = 1.64; 95% CI: 1.10-2.54). Furthermore, the combined IVS12-6CC and IVS10+12AA genotypes also significantly increased the risk of GC (OR = 2.12, 95% CI: 1.22-3.66). Statistically significant interactions were observed between: IVS10+12G> A and drinking, high pickled food or fried food intake (OR = 2.32; 95% CI: 1.43-3.78, OR = 2.55; 95% CI: 1.48-4.21 and OR = 2.88; 95% CI: 1.70-4.94, respectively); and IVS12-6T> C and high pickled food intake or fried food intake (OR = 2.65; 95% CI: 1.62-4.47 and OR = 2.48; 95% CI: 1.42-4.13, respectively). Conclusion: The IVS10+12G> A and IVS12-6T> C polymorphisms in MSH2 gene appear to be associated with risk of GC in this Chinese population. Risk for GC, stratified by related genotypes, was further modified by drinking, high pickled food or fried food intake. Larger prospective studies are needed to confirm these findings. © 2012 Elsevier Ltd. Source
Wu J.,Institute of Cancer Research of Jiangsu Province |
Wang D.,Nanjing Medical University |
Song L.,Nanjing Medical University |
Li S.,Institute of Cancer Research of Jiangsu Province |
And 5 more authors.
Molecular Biology Reports
We designed to understand the effects of the T1151A gene polymorphism in the hMLH1 gene on the pathogenesis of familial gastric cancer. Peripheral blood DNA from 113 patients with familial gastric cancer or suspected familial gastric cancer that were newly identified in the same year, along with 180 healthy subjects, was subjected to polymerase chain reaction-denaturing highperformance liquid chromatography (PCR-DHPLC) and DNA sequencing of exon 12 in the hMLH1 gene. Our results as following, the T1151A detection rate was remarkably higher in patients with familial gastric cancer or suspected familial gastric cancer compared to normal control patients (P<0.05). Stratified analysis showed that there was a significant difference in the detection rate between the control group and elderly patients whose age of onset was greater than 50 years old (P<0.05). The detection rate of patients from high-risk families were relatively high (P<0.05). An especially significant distribution was observed in patients who had suffered precancerous diseases related to gastric cancer (P<0.01). In conclusion, familial gastric carcinoma families in China carrying the T1151A polymorphism may have a higher risk of suffering from gastric cancer. This gene polymorphism can be used as a candidate screening index for high-risk populations. © Springer Science+Business Media B.V. 2010. Source