Institute of Cancer Research and Prevention of Jiashan County

Jiashan, China

Institute of Cancer Research and Prevention of Jiashan County

Jiashan, China
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Yu Y.,Zhejiang University | Zhang M.,Zhejiang University | Pan Y.,Zhejiang University | Jin M.,Zhejiang University | And 6 more authors.
International Journal of Colorectal Disease | Year: 2012

Purpose The primary aim was to respectively evaluate PLA2G4A mutants modifying protective effect of tea consumption against colorectal cancer (CRC), colon and rectal cancer. Methods All participants were recruited from January 2006 to April 2008. The information about tea consumption was collected by a structured questionnaire. CRC patients were diagnosed based on histology. Four single-nuclear polymorphisms (SNPs) in PLA2G4A gene were selected. Multiple logistic regression models were used for assessing the joint effects between tea consumption and SNPs on CRC, colon and rectal cancer. Results Three hundred patients with CRC and 296 controls well-matched were used in the final analyses. The significant individual associations between four SNPs (rs6666834, rs10911933, rs4650708 and rs7526089) and CRC were not observed. However, their CTAC haplotype was significantly associated with the increased risk of CRC (OR03.06; 95% CI01.52-6.19), compared with TCAC haplotype. Drinking tea was correlated with a decreased risk of CRC after adjustment for covariates (OR00.61; 95%CI00.39-0.97). Meanwhile, compared with no-tea drinkers with TT/CT genotype of rs6666834, tea drinkers with TT/CT or CC had significant lower risk of CRC (OR00.6, 95%CI0 0.36-1.00 for TT/CT; 0.38, 0.19-0.74 for CC). The joint effects between the remaining three SNPs and drinking tea on CRC were observed as well. Similar findings were observed on colon and rectal cancers. Conclusions Tea consumption and haplotype of mutants in PLA2G4A gene were respectively associated with the risk of CRC. PLA2G4A mutants modified the protective effect of tea consumption against CRC, colon and rectal cancers in Chinese population. © Springer-Verlag 2012.


Yu Y.,Zhejiang University | Pan Y.,Zhejiang University | Jin M.,Zhejiang University | Zhang M.,Zhejiang University | And 6 more authors.
International Journal of Colorectal Disease | Year: 2012

Purpose This study aims to explore the associations of polymorphisms in tachykinin, precursor 1 (TAC1), tachykinin receptor 1 (TACR1), and tachykinin receptor 2 (TACR2) genes and their interactions with the risk of colorectal cancer (CRC) among Chinese population. Methods A population-based case-control study which included 394 cases and 393 cancer-free controls was carried out. A total of 19 tagSNPs in the three genes were chosen based on HapMap and NCBI datasets and genotyped by SNPshot assay. Multiple logistic regression models were applied to evaluate the associations of SNPs with CRC after adjustment for potential covariates. Furthermore, generalized multifactor dimensionality reduction (GMDR) method was used to test the interactive effect among three genes on CRC. Results Compared with those carrying rs3755457 CC/CT or rs12477554 TT/CT genotype, individuals carrying homozygous variants had higher risk of colorectal cancer (adjusted OR01.80, 95 % CI01.03-3.13, P00.039 for rs3755457; adjusted OR01.73, 95 % CI01.07-2.79, P00.024 for rs12477554). As for rs10198644, GG genotypewas associated with a 1.72-fold (95 % CI00.37-0.88) decreased risk when compared with the common CC genotype. Moreover, the GMDR analysis indicated that the best interactive model included five polymorphisms: rs2072100 (TAC1), rs10198644 (TACR1), rs2193409 (TACR1), rs3771810 (TACR1), and rs4644560 (TACR2). Conclusions Our study suggests that tachykinins pathway genes may participate in the development of CRC and the potential interactions among the three genes on CRC may exist, which has to be confirmed in future larger studies. © Springer-Verlag 2012.


Liu H.,Zhejiang University | Jiang X.,Zhejiang University | Zhang M.-W.,Zhejiang University | Pan Y.-F.,Zhejiang University | And 5 more authors.
Journal of Zhejiang University: Science B | Year: 2013

The initiators caspase-9 (CASP9) and caspase-10 (CASP10) are two key controllers of apoptosis and play important roles in carcinogenesis. This study aims to explore the association between CASPs gene polymorphisms and colorectal cancer (CRC) susceptibility in a population-based study. A two-stage designed population-based case-control study was carried out, including a testing set with 300 cases and 296 controls and a validation set with 206 cases and 845 controls. A total of eight tag selected single nucleotide polymorphisms (SNPs) in CASP9 and CASP10 were chosen based on HapMap and the National Center of Biotechnology Information (NCBI) datasets and genotyped by restriction fragment length polymorphism (RFLP) assay. Multivariate logistic regression models were applied to evaluate the association of SNPs with CRC risk. In the first stage, from eight tag SNPs, three polymorphisms rs4646077 (odds ratio (OR) AA+AG: 0.654, 95% confidence interval (CI): 0.406-1.055; P=0.082), rs4233532 (ORCC: 1.667, 95% CI: 0.967-2.876; ORCT: 1.435, 95% CI: 0.998-2.063; P=0.077), and rs2881930 (ORCC: 0.263, 95% CI: 0.095-0.728, P=0.036) showed possible association with CRC risk. However, none of the three SNPs, rs4646077 (ORAA+AG: 1.233, 95% CI: 0.903-1.683), rs4233532 (ORCC: 0.892, 95% CI: 0.640-1.243; ORCT: 1.134, 95% CI: 0.897-1.433), and rs2881930 (ORCC: 1.096, 95% CI: 0.620-1.938; ORCT: 1.009, 95% CI: 0.801-1.271), remained significant with CRC risk in the validation set, even after stratification for different tumor locations (colon or rectum). In addition, never tea drinking was associated with a significantly increased risk of CRC in testing set together with validation set (OR: 1.755, 95% CI: 1.319-2.334). Our results found that polymorphisms of CASP9 and CASP10 genes may not contribute to CRC risk in Chinese population and thereby the large-scale case-control studies might be in consideration. In addition, tea drinking was a protective factor for CRC. © 2013 Zhejiang University and Springer-Verlag Berlin Heidelberg.


Wu Y.,Zhejiang University | Jin M.,Zhejiang University | Liu B.,Zhejiang University | Liang X.,Zhejiang University | And 5 more authors.
Molecular Carcinogenesis | Year: 2011

The xeroderma pigmentosum complementation group C (XPC) is responsible for removal of bulky helix-distorting DNA lesions. Several polymorphisms of XPC gene may modulate the colorectal cancer (CRC) susceptibility. We assessed the association of XPC Lys939Gln (A/C), Ala499Val (C/T), and PAT (-/+) polymorphisms with CRC risk in a population-based case-control study which included 421 CRC patients and 845 controls. For Lys939Gln, the CC genotype was associated with a significantly increased risk of CRC (odds ratio (OR)=1.5; 95% confidence interval (CI)=1.0-2.2) compared with the AA genotype. The subjects with PAT +/+ genotype had a significantly increased risk of CRC (OR=1.5; 95% CI=1.0-2.3), compared with those with PAT-/- genotype. Though no significant association between Ala499Val and CRC risk was observed, we found that individuals carrying the CT+TT genotypes showed a significantly decreased risk of rectal cancer (OR=0.7; 95% CI=0.5-1.0). Additionally, the haplotype C+C was associated with a significantly increased CRC risk (OR=1.3; 95% CI=1.0-1.6), compared with the most common haplotype A-T. Further, individuals with four or more risk alleles exhibited a significantly increased risk of CRC (OR=1.4; 95% CI=1.0-2.0), with a significant gene-dosage effect (P for trend=0.038). Besides, never tea drinking was associated with a significantly increased risk of CRC (OR=2.3; 95% CI=1.7-3.3). Our results suggest that the XPC polymorphisms may modulate CRC susceptibility independently or jointly, and tea drinking has a protective effect on CRC. © 2010 Wiley-Liss, Inc.


Yu Y.,Zhejiang University | Liu H.,Zhejiang University | Jin M.,Zhejiang University | Zhang M.,Zhejiang University | And 4 more authors.
Annals of Human Genetics | Year: 2012

Due to the high morbidity and mortality of colorectal cancer (CRC), this study aims to determine the joint association of RE-1-silencing transcription factor (REST) and nuclear factor-κB 1 (NFKB1) genes with CRC in a population-based study. A well-matched case-control study including 390 controls and 388 patients with CRC was enrolled in China. The selected single nucleotide polymorphisms (SNPs) in the REST and NFKB1 genes were genotyped by Illumina SnapShot Chip. After adjustment for important covariates, the associations of SNPs and joint association of REST and NFKB1 with CRC were evaluated by multiple logistic regression models. The subjects with the rs2228991 AA genotype of the REST gene had a decreased risk for CRC (OR = 0.38; 95%CI: 0.19-0.74), compared with the GG genotype. There were no significant associations between three SNPs in the NFKB1 gene, their haplotype and CRC risk. However, a significant combined effect of rs3774959 and rs3774964 in the NFKB1 gene with rs2228991 in the REST gene on CRC risk was observed. In conclusion, the present study found that mutation in the REST gene rather than the NFKB1 gene was associated with the risk of CRC. Furthermore, significant REST-NFKB1 joint association was observed for CRC, colon cancer and rectal cancer risk. © 2012 Blackwell Publishing Ltd/University College London.


Liu B.,Zhejiang University | Zhang Y.,Zhejiang University | Jin M.,Zhejiang University | Ni Q.,Zhejiang University | And 5 more authors.
Molecular Carcinogenesis | Year: 2010

It has been well elucidated that the signal transduction of cell-cycle control pathway and apoptosis pathway plays an important role in the normal growth and differentiation of organisms. To test the hypothesis that mutants of key genes involved in cell-cycle regulation and apoptosis might contribute to the increased risk of colorectal cancer (CRC), a population-based case-control study was carried out in Jiashan County, Zhejiang Province. The study population was composed of 373 CRC cases and 838 controls. Five genetic variants including CCND1 G870A, p21 codon31 C/A, p21 3′UTR C/T, caspase8 IVS12-19G/A, and caspase8 6n del/ins were genotyped. The associations of the polymorphisms with CRC were estimated by logistical regression model after adjustment for the important covariates. The interactive effect among the five selected genetic polymorphisms on CRC was explored by multifactor dimensionality reduction (MDR) software. The significant association between five single-nucleotide polymorphisms (SNPs) and CRC risk was not observed, respectively. However, caspase8 del/del showed a marginally significant association with the increased risk of rectum cancer [adjusted odds ratio (OR) (95% confidence interval, CI)=1.92 (0.97-3.79); P=0.06]. Furthermore, the MDR analysis indicated that the best interactive model for CRC included three factors - CCND1 G870A, caspase8 IVS12-19G/A, and caspase8 6 n del/ins - with 53.44% testing balanced accuracy and 10/10 cross-validation consistency, but the model was no longer significant after the 1000 times permutation test (P=0.25). Our findings suggest that the selected polymorphisms of p21, CCND1, and caspase8 may not contribute to the risk of colorectal cancer. © 2009 Wiley-Liss, Inc.


Ma X.,Institute of Cancer Research and Prevention of Jiashan County | Li Q.,Institute of Cancer Research and Prevention of Jiashan County | Ma W.,Institute of Cancer Research and Prevention of Jiashan County
Chinese-German Journal of Clinical Oncology | Year: 2012

Objective: The aim of our study was to estimate the cost of colorectal cancer screening and to provide evidence for the cost control of colorectal cancer screening among general population in rural area of China. Methods: We determined the net cost for colorectal cancer mass-screening in Jiashan County, and evaluated the cost-benefit and cost effectiveness. Results: The compliance rate of primary screening and intensive screening were 84.6% and 78.7%, respectively. In primary screening, the average cost for each individual was 27.2 yuan, and the average cost for identifying one high-risk individual was 180.5 yuan. The mean cost to diagnose one colorectal cancer patient was 42963.3 yuan. As for identification of adenoma, the average cost for each case was 4384.0 yuan. Based on the calculation, the average cost of reducing one colorectal cancer patient was 12768 yuan by conducting the mass-screening protocol. Conclusion: It was beneficial to do the cost-benefit analysis of colorectal cancer screening in area of high incidence. Based on the results of cost-benefit analysis, more efforts should be made to reduce the cost and to improve the efficiency of the colorectal cancer screening. © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2012.

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