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Cardiff, United Kingdom

Ge Z.,Institute of Cancer and Genetics | Ge Z.,Capital Medical University | Sanders A.J.,Institute of Cancer and Genetics | Ye L.,Institute of Cancer and Genetics | And 2 more authors.
Oncology Reports | Year: 2013

Death receptor-3 (DR3) plays controversial roles in cancer. Currently, DR3 is known to be a functional receptor of vascular endothelial growth inhibitor (VEGI). The role of DR3 in breast cancer remains unclear. The present study investigated DR3 expression in a clinical cohort of breast cancer patients and its role in breast cancer cells in vitro. The expression of DR3 was examined in a breast cancer cohort using quantitative PCR (Q-PCR) and immunohistochemistry (IHC) in comparison to the patients' data. In vitro function of DR3 was examined through the targeting of this molecule in MCF7 and MDA-MB-231 breast cancer cells using ribozyme transgene technology. Decreased DR3 expression was noted in breast cancer tissues compared to normal tissues and decreased expression of DR3 was generally associated with a poorer prognosis as well as a significantly shorter long-term survival (p=0.038). Targeting of DR3 in vitro in breast cancer cell lines resulted in impaired migratory rates compared to respective control cells. Collectively, these data suggest a complex role for DR3 in breast cancer development and progression. © 2013 Spandidos Publications Ltd. All rights reserved. Source

Du P.,University of Cardiff | Du P.,Institute of Cancer and Genetics | Du P.,Capital Medical University | Ye L.,University of Cardiff | And 6 more authors.
Journal of Experimental Therapeutics and Oncology | Year: 2012

Background: Metastasis suppressor 1 (MTSS1), a cytoskeletal associated protein, has been indicated in certain types of human cancers, but its role in kidney cancer remains unknown. We have investigated the expression of MTSS1 in normal and malignant human kidney tissues and its molecular interaction within kidney cancer cells. Materials and Methods: The expression of MTSS1 in human kidney tissues and kidney cancer cell lines was assessed at both the mRNA and protein levels using RTPCR and immunohistochemistry, respectively. Full-length MTSS1 cDNA expression vector was used to generate MTSS1 over-expressing cells. Effect of MTSS1 overexpression on cellular functions, was examined in kidney cancer cells of MTSS1 being over-expressed using a variety of in vitro assays. Involvement of Sonic Hedgehog (SHH) pathway was tested by using Shh small inhibitors. Results: Epithelial cells at proximal tubules of kidney tissues were stained positively for MTSS1, while the staining was weak or absent from cells at corpuscles and cancer cells of tumour tissues. Similarly, in kidney cancer cell lines, CAKI-2 and UMRC-2, expressed very low level of MTSS1. Over-expression of MTSS1 reduced the growth, invasion, adhesion and migration of kidney cell lines in vitro. Shh inhibitors diminished the inhibitory effect of MTSS1 on cell migration. Conclusion: MTSS1 expression is reduced in human kidney cancer cells. MTSS1 levels are inversely correlated with the growth, invasion, adhesion and migration of kidney cancer cells in vitro. MTSS1 suppresses migration of kidney cancer cells via SHH pathway, and is a putative tumour suppressor of kidney cancer. © 2012 Old City Publishing, Inc. Source

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