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Chongqing, China

Luo Y.-Q.,CAEP - China Academy of Engineering Physics | Huang L.-X.,CAEP - China Academy of Engineering Physics | Yang J.-J.,Institute of Burn Research | Shen Z.-X.,CAEP - China Academy of Engineering Physics | And 2 more authors.
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2012

Thermal injuries are a serious medical problem in the China. The accurate determination of burn degree is difficult for scatheless diagnose and a precondition of treating burn wounds. Multi-spectral photographic analysis is expected to play an important role in determining burn wound degree, the Liquid Crystal Tunable Filter has a capability of selecting the observing wavelength instaneously with high spectral resolution and excellent imaging quality in visible and near-infrared spectrum band. Taking advantage of this filter, we have developed a LCTF imaging spectrometer prototype instrument at visible wavelength bands for burn wound diagnose. In this paper, spectral analysis experiments were first performed on KUNMING mice and burn injury patients to find the characteristic reflective spectral curves at 400nm-1800nm, the imaging spectrometer prototype instrument using LCTFs which are sensitive to radiation in 420nm-750nmwavelength bands was built based on spectral analysis results. The spectral imaging experiments on burn injury patients have verified the excellent properties of the prototype instrument, including high quality spectral images with spectral resolution of less than 7nm and continuous selection of the output wavelength. The burn areas of patients were marked with different colors which represents as different burn degree and the spectral imaging system has thus been proven to have the ability to classify the burn areas through comparing their reflective spectral curves with characteristic spectrum of the different burn degree in spectral database in the future. Finally, the application of the LCTF imaging spectrometer to burn wound diagnose are summarized based on the results of spectral imaging experiments on burn injuries. © 2012 SPIE.

Song H.-P.,Institute of Burn Research | Zhang L.,Chongqing Medical University | Dang Y.-M.,Institute of Burn Research | Yan H.,Institute of Burn Research | And 2 more authors.
Clinical and Experimental Pharmacology and Physiology | Year: 2010

1. After a severe burn, a marked decrease in myocardial blood flow results in ischaemic and hypoxic injury, which subsequently leads to apoptosis or necrosis. Phosphatidylinositol 3-kinase (PI3-K)/Akt is an important intracellular signal transduction molecule that regulates cell proliferation, differentiation, glucose metabolism and migration. However, the function and mechanisms of the PI3-K-Akt pathway in cardiomyocyte apoptosis after a burn remain unclear. 2. In the present study, an in vivo rat model of burn injury and an in vitro hypoxic model using rat cardiomyocytes were established. In burned rats, the expression of PI3-K and phosphorylated (p-) Akt expression increased, as did myocardial apoptosis. Inhibition of the PI3-K-Akt pathway with 1.4 mg/kg LY294002 caused a significant increase in the myocardial apoptotic index compared with hypoxia alone in the in vivo model. 3. Cardiomyocytes cultured under hypoxic conditions exhibited increased apoptosis, decreased cell viability, enhanced caspase 3 activity, a decreased mitochondrial membrane potential, increased cytoplasmic calcium transients and increased p53 and Bax mRNA expression. Pretreatment with 50 μmol/L LY294002 significantly enhanced all these negative indicators compared with hypoxia alone. In contrast, pretreatment of cells with 200 ng/mL insulin-like growth factor-1, an activator of PI3-K-Akt, significantly ameliorated the effects of hypoxia, although control levels were not reached. 4. These findings indicate that activation of the PI3-K-Akt pathway induced by ischaemia and hypoxia after a severe burn can protect cardiomyocytes from apoptosis. This anti-apoptotic effect is most likely mediated via the mitochondria and changes in p53 and Bax gene expression, intracellular [Ca2+] and caspase 3 activity. © 2010 Blackwell Publishing Asia Pty Ltd.

Jiang X.,Institute of Burn Research | Zhang J.,Institute of Burn Research | Huang Y.,Institute of Burn Research
Cell Adhesion and Migration | Year: 2015

Tetraspanins are a superfamily of small transmembrane proteins that are expressed in almost all eukaryotic cells. Through interacting with one another and with other membrane and intracellular proteins, tetraspanins regulate a wide range of proteins such as integrins, cell surface receptors, and signaling molecules, and thereby engage in diverse cellular processes ranging from cell adhesion and migration to proliferation and differentiation. In particular, tetraspanins modulate the function of proteins involved in all determining factors of cell migration including cell–cell adhesion, cell–ECM adhesion, cytoskeletal protrusion/contraction, and proteolytic ECM remodeling. We herein provide a brief overview of collective in vitro and in vivo studies of tetraspanins to illustrate their regulatory functions in the migration and trafficking of cancer cells, vascular endothelial cells, skin cells (keratinocytes and fibroblasts), and leukocytes. We also discuss the involvement of tetraspanins in various pathologic and remedial processes that rely on cell migration and their potential value as targets for therapeutic intervention. © 2015 Taylor & Francis Group, LLC.

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