Ruiz-Herrera A.,Autonomous University of Barcelona |
Ruiz-Herrera A.,Institute Of Biotecnologia I Biomedicina |
Farre M.,Autonomous University of Barcelona |
Ponsa M.,Autonomous University of Barcelona |
Robinson T.J.,Stellenbosch University
Chromosome Research | Year: 2010
Monobrachial homology resulting from Robertsonian (Rb) fusions is thought to contribute to chromosomal speciation through underdominance. Given the karyotypic diversity characterizing wild house mouse populations [Mus musculus domesticus, (MMU)], variation that results almost exclusively from Rb fusions (diploid numbers range from 22 to 40) and possibly whole arm reciprocal translocations (WARTs), this organism represents an excellent model for testing hypotheses of chromosomal evolution. Previous studies of chromosome size and recombination rates have failed to explain the bias for certain chromosomes to be involved more frequently than others in these rearrangements. Here, we show that the pericentromeric region of one such chromosome, MMU19, which is infrequently encountered as a fusion partner in wild populations, is significantly enriched for housekeeping genes when compared to other chromosomes in the genome. These data suggest that there is selection against breakpoints in the pericentromeric region and provide new insights into factors that constrain chromosomal reorganizations in house mice. Given the anticipated increase in vertebrate whole genome sequences, the examination of gene content and expression profiles of the pericentromeric regions of other mammalian lineages characterized by Rb fusions (i.e., other rodents, bats, and bovids, among others) is both achievable and crucial to developing broadly applicable models of chromosome evolution. © 2010 Springer Science+Business Media B.V.
Carne-Sanchez A.,Institute Catala Of Nanociencia I Nanotecnologia |
Bonnet C.S.,CNRS Center for Molecular Biophysics |
Imaz I.,Institute Catala Of Nanociencia I Nanotecnologia |
Lorenzo J.,Institute Of Biotecnologia I Biomedicina |
And 3 more authors.
Journal of the American Chemical Society | Year: 2013
The macrocyclic ligand DOTP is used to assemble a porous, heterometallic metal-organic framework (MOF). This MOF is miniaturizable down to the nanoscale to form stable colloids, is stable in physiological saline solution and cell culture media, and is not cytotoxic. It shows interesting relaxometric properties with r1 at high field (500 MHz) of 5 mM -1·s-1 and a maximum r1 = 15 mM -1·s-1 at 40 MHz, which remains constant over a wide pH range and increases with temperature. © 2013 American Chemical Society.
Lluch-Senar M.,Institute Of Biotecnologia I Biomedicina |
Lluch-Senar M.,Autonomous University of Barcelona |
Querol E.,Institute Of Biotecnologia I Biomedicina |
Querol E.,Autonomous University of Barcelona |
And 2 more authors.
Molecular Microbiology | Year: 2010
Mycoplasma genomes exhibit an impressively low amount of genes involved in cell division and some species even lack the ftsZ gene, which is found widespread in the microbial world and is considered essential for cell division by binary fission. We constructed a Mycoplasma genitalium ftsZ null mutant by gene replacement to investigate the role of this gene and the presence of alternative cell division mechanisms in this minimal bacterium. Our results demonstrate that ftsZ is non-essential for cell growth and reveal that, in the absence of the FtsZ protein, M. genitalium can manage feasible cell divisions and cytokinesis using the force generated by its motile machinery. This is an alternative mechanism, completely independent of the FtsZ protein, to perform cell division by binary fission in a microorganism. We also propose that the mycoplasma cytoskeleton, a complex network of proteins involved in many aspects of the biology of these microorganisms, may have taken over the function of many genes involved in cell division, allowing their loss in the regressive evolution of the streamlined mycoplasma genomes. © 2010 Blackwell Publishing Ltd.
Prat E.,Institute Of Biotecnologia I Biomedicina |
del Rey J.,Institute Of Biotecnologia I Biomedicina |
Ponsa I.,Institute Of Biotecnologia I Biomedicina |
Nadal M.,Hospital Duran i Reynals |
And 6 more authors.
Urology | Year: 2010
Objectives: To classify bladder tumors according to their genomic imbalances and evaluate their association with patient's outcome. Methods: Sixty-three superficially and minimally invasive bladder tumors were analyzed by conventional comparative genomic hybridization. Subtelomeric screening in 15 of these tumors was performed by multiplex ligation-dependent probe amplification. Results: Losses of 9q and 9p (32% and 25% of all cases, respectively) as well as gains of chromosomes Xq and Xp (28% and 25%, respectively) were the most frequent chromosome imbalances. Losses of 8p and gains in 1q and 8q were detected in >20% of cases. Tumors were classified into 3 groups according to their individualized pattern of gains and losses. The largest group was characterized by few chromosome imbalances, presenting 77% and 49% of the Ta and T1 tumors, respectively. Another group characterized by chromosomal gains, was composed of equal number of Ta and T1 tumors, with +1q and +17q gains being the most common imbalances. A minority group was characterized by chromosomal losses on 11q, 5q, and 6q. The multiplex ligation-dependent probe amplification study showed good correlation with comparative genomic hybridization results. With regard to the biological significance of this classification, a remarkable fact is that this minority group composed mainly of T1 tumors, showed a significant decrease in patient overall survival. Conclusions: Our data suggest that superficial carcinomas of the bladder can be subdivided into a larger number of subclasses than had previously been expected. Our results also demonstrate a decreased survival among patients whose tumors show more genomic losses than gains. Crown Copyright © 2010.