Institute of Biotechnology and Allied science

Sīkar, India

Institute of Biotechnology and Allied science

Sīkar, India
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Singh R.,Institute of Biotechnology and Allied science
Journal of Chemical and Pharmaceutical Research | Year: 2011

Each year, the chickpea suffer's from significant losses in both quantity and quality. These losses are observed particularly in stocks, which are principally due to attacks by weevils. The most devastating is the Chinese weevil (Callosobruchus chinensis). In the context of biological control as an alternative to chemical control, three plants were tested for their possible role organic insecticide, namely Thymus vulgarus, Santolina chamaecyparissus and Anagyris foetida. The leaf powders and oils of these plants that was extracted by hydro distillation were tested for their effect on the biology of C. chinensis such as fertility, longevity and sex ratio. The results showed that oils and powders of three different plants tested possess insecticidal activity against C. chinensis. By comparing these oils, thyme oil at a dose of 10 μl exhibited great biocidal power against insects as we experienced 100% mortality after only hours of exposure to treatment, hard tan l 'oil santolina also had 100% mortality but at a dose of 20 μl. Regarding the powder, that of Santoline when compared to other powders at a dose of 1 g significantly reduces longevity (2.9 days), fecundity (3.06) and fertility (3.33). It even has an ovicidal effect. For 10 repetitions, we have recorded a single outbreak. This allows us to move forward so that the oils and powders tested plants can be used as bio insecticide for control of C. chinensis populations of pests of stored foodstuffs.


Rathore M.,Institute of Biotechnology and Allied science | Sharma N.,Indian Agricultural Research Institute
Natural Products Journal | Year: 2011

Since most of the pathogenic microbes have developed resistance against chemical drug derivatives therefore need of the hour is to formulate plant based natural medicines. These formulations with no or least toxicity can prove better alternative of synthetic drugs. Ethnopharmacologists, botanists, microbiologists, and natural-products chemists are combing the earth for phytochemicals and "leads" which could be developed for the treatment of infectious diseases. While, 25 to 50% of current pharmaceuticals are derived from plants, none are used as antimicrobials. Traditional healers have long used plants to prevent or cure infectious conditions; western medicine is trying to duplicate their successes. Plants are rich in a wide variety of secondary metabolites, such as tannins, terpenoids, alkaloids, and flavonoids, which have been found in vitro to have antimicrobial properties. The present review mainly discusses exploitation of plant secondary compounds as antimicrobial agents. Mode of action of some agents was also addressed. Since many of these compounds are currently available as unregulated botanical preparations and their use by the public is increasing rapidly, clinicians need to consider the consequences of patients self-medicating with these preparations. © 2011 Bentham Science Publishers.


Malhotra A.,Institute of Biotechnology and Allied science | Sharma M.,Institute of Biotechnology and Allied science | Sharma D.,Jaipur Dental College
International Journal of Pharma and Bio Sciences | Year: 2012

Honey is a natural sweetener, but it is not just a sweetener it's a nature's gift to mankind. Natural honey has various ingredients in it, that contribute to its incredible properties. It's antimicrobial properties have attracted researchers towards itself and now we can find many research papers published on this topic. Honey is normally used in our daily life for treatment of hearing loss, bad breath, fatigue, weight loss, pimples, influenza, ingestion, heart diseases, toothache, hair loss, bladder infections, infertility etc. Honey is used as a mixture with many natural products such as lemon, clover, milk, cinnamon and water for treatment of various ailments and other health disorders. Beside this, honey is now used in various industries that exploit nature's wonderful gift (honey). Commercially honey is used as moisturizer, hair conditioner, laxative, aphrodisiac, rooting hormone, cleansers etc. Honey mixed with ground almonds makes an excellent facial cleansing scrub.


Sharma N.,Institute of Biotechnology and Allied science | Rathore M.,Institute of Biotechnology and Allied science | Sharma M.,Institute of Biotechnology and Allied science
Reviews in Environmental Science and Biotechnology | Year: 2013

Today pectinases are upcoming industrially important bacterial enzymes. It can be produced by a variety of microorganisms. These enzymes act on pectin, which is the major component of middle lamella in plant cell wall. Pectinolytic enzymes are classified according to their mode of attack on the galacturonan part of the pectin molecules such as protopectinases, esterase's and depolymerases. As we know that microbial enzymes work depends up on the type of enzymes application, temperature, concentration, and pH and so on, therefore, pectinase enzyme also differentiated according to their physical and chemical factors too. The biochemical structures of pectinases include members of all the major classes and the structure-function relationship, studies of a few available complexes of pectinases with substrate/analogs could be considered as prototypes for related family member and the molecular characterization of pectinolytic enzymes is also well documented. Furthermore, it provides a bird's eye view of the possible application of these enzymes in commercial sector. © 2012 Springer Science+Business Media B.V.

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