Institute of Bioresources and Sustainable Development IBSD

Imphal, India

Institute of Bioresources and Sustainable Development IBSD

Imphal, India
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Sahoo D.,Institute of Bioresources and Sustainable Development IBSD | Ummalyma S.B.,Institute of Bioresources and Sustainable Development IBSD | Okram A.K.,Institute of Bioresources and Sustainable Development IBSD | Sukumaran R.K.,Indian National Institute for Interdisciplinary Science and Technology | George E.,Indian National Institute for Interdisciplinary Science and Technology
Bioresource Technology | Year: 2017

The aim of present study was to evaluate feasibility of using the Para grass as feedstock for production of bioethanol. Process involved the pretreatment with dilute acid or alkali and followed by enzymatic saccharification with commercial cellulase. Maximum sugar release of 696. mg/g was obtained from 10% biomass loading and 0.5% w/v of alkali whereas in the case of acid pretreatment maximum sugar of 660. mg/g was obtained from 20% biomass loading and 2% w/v acid loading. Results showed that Para grass utilization as a biorefinery feedstock can be a potential strategy to address the sustainable utilization of this invasive grass thereby keeping its population in check in the Loktak Lake. © 2017 Elsevier Ltd.

Ummalyma S.B.,Institute of Bioresources and Sustainable Development IBSD | Gnansounou E.,Ecole Polytechnique Federale de Lausanne | Sukumaran R.K.,Indian National Institute for Interdisciplinary Science and Technology | Sindhu R.,Indian National Institute for Interdisciplinary Science and Technology | And 2 more authors.
Bioresource Technology | Year: 2017

Microalgae based research has been extensively progressed for the production of value added products and biofuels. Potential application of microalgae for biofuel is recently gained more attention for possibilities of biodiesel and other high value metabolites. However, high cost of production of biomass associated with harvesting technologies is one of the major bottleneck for commercialization of algae based industrial product. Based on the operation economics, harvesting efficiency, technological possibilities, flocculation of algal biomass is a superior method for harvesting microalgae from the growth medium. In this article, latest trends of microalgal cell harvesting through flocculation are reviewed with emphasis on current progress and prospect in environmental friendly bio-based flocculation approach. Bio-flocculation based microalgae harvesting technologies is a promising strategy for low cost microalgal biomass production for various applications. © 2017 Elsevier Ltd.

Biswas D.,Sri Krupa Institute of Pharmaceutical Science | Yoganandam G.P.,Government of Puducherry | Dey A.,Institute of Bioresources and Sustainable Development IBSD | Deb L.,Institute of Bioresources and Sustainable Development IBSD
Indian Journal of Pharmaceutical Sciences | Year: 2013

To rationalize scientifically the traditional claim on use of Wedelia biflora (Linn.) D. C. for the treatment of wounds and infections, the present study was designed to evaluate the antimicrobial and wound healing activity of ethanol extract of leaves of W. biflora. In in vitro assays the test extract was subjected to antimicrobial activity by agar well-diffusion method and minimum inhibitory concentration method in different microbial strains. Wound healing activity of the test extract was studied by excision wound model and incision wound model in Wistar albino rats. In excision wound model, 97.90% wound healing was recorded in 10% w/w extract treated group on 16 th days of postsurgery, whereas only 58.50% was observed in control group. In incision model, higher breaking strength, high hydroxyl proline content and histopathological study in extract treated groups revealed higher collagen redeposition than the control group. The agar well-diffusion evaluation and minimum inhibitory concentration established antimicrobial efficacy of ethanol extracts of W. biflora. These observations established the traditional claim and therapeutic activity of W. biflora and it could be a potent wound healing candidate for use in future.

Lokesh D.,Institute of Bioresources and Sustainable Development IBSD | Amitsankar D.,R K Pharmacy College
Journal of Ethnopharmacology | Year: 2012

Ethnopharmacological relevance: The present investigation was aimed to justify the pharmacological basis in traditional use of Clerodendrum colebrookianum as antihypertensive agent in north-east India. Materials and Methods: The aqueous extract (AECc), its aqueous, n-butanol (nBFCc), Ethyl-acetate (EtFCc) and Chloroform fractions of C. colebrookianum leaves were evaluated for antihypertensive potential by using fructose-induced hypertension model in rats and in isolated frog heart. The ex-vivo muscarinic action in isolated rat ileum, in-vitro assay for Rho-kinase (ROCK -II), phosphodiesterase-5 (PDE-5) and angiotension converting enzyme (ACE) were also carried out to establish the mechanism of action of samples. The total phenolic and flavonoied contents in test samples were estimated to establish phyto-pharmacological relationship. Results: The 100 μg/mL test samples were showed calcium antagonism in rat ileum and at 50 μg/mL and 75 μg/mL doses exhibited ROCK-II and PDE-5 inhibition respectively where, EtFCc was caused maximum 68.62% (ROCK-II) and 52.28% (PDE-5) inhibition, but none of the sample was exhibit effect in ACE at 100 μg/mL. The test samples also showed negative inotropic and chronotropic effect on isolated frog heart and significant (P<0.001) reduction in systolic blood pressure and heart rate in hypertensive rats compared to control. The total phenolic content maximum 80 μg gallic acid equivalents in nBFCc and flavonoids content maximum 69.57 μg Quercetin equivalent in AECc were estimated. Conclusions: These observations established the traditional claim and thus C. colebrookianum could be a potent antihypertensive agent for use in future. The antihypertensive effect mediated by cholinergic action and following ROCK - II, PDE-5 inhibition of C. colebrookianum. © 2012 Elsevier Ireland Ltd.

Lokesh D.,Institute of Bioresources and Sustainable Development IBSD | Amitsankar D.,Rk Pharmacy College
Pharmacognosy Journal | Year: 2012

North-East india is in two biodiversity hotspots (Indo-Burma & Himalayan) in world map and the indigenous medicinal plants grown in the North - East India are useful folk medicines used by the people of this region. Prunus persica (L) Batsch and Clerodendrum colebrookianum Walp widely distributed in North-East India are popular folk medicine for the treatment of hypertension. The present investigation deals with the qualitative and quantitative microscopic evaluation of the leaf material and establishment of its quality parameters, including physicochemical and phytochemical evaluation. Chief characters of transverse section include primary and secondary vascular bundles surrounded by single layer endidermal cells in both the plants. In microscopic characters it is found that the anisocytic stomata surrounded by three subsidiary cells of which one markedly smaller than other in lower epidermal, but no stomata was found in upper epidermal of Prunus persica and the anomocytic stomata surrounded by varying number of cells in both upper and lower epidermis. The diagnostic characters of powders viz. sclerencnymatous fibres, sptocrophide or calcium oxalate crystals, starch grains, xylem vessels were found in Prunus persica and trichome, xylem vessel, phloem fiber, lignified fiber, epidermal cell in Clerodendrum colebrookianum. Various physicochemical parameters were also established. Phytochemical screening revealed the presence of many therapeutically important classes of phytoconstituents such as reducing sugar, proteins, steroid, cardiac-glycoside, flavonoids, tannins and saponins. The present investigation was also aimed at justifying the antioxidant potential of the leaves extracts of the plant that may contribute antihypertensive activity of plant.

Suthar S.K.,Jaypee University of Information Technology | Sharma N.,BAHRA University | Lee H.B.,Cancer Research Initiative Foundation | Nongalleima K.,Institute of Bioresources and Sustainable Development IBSD | Sharma M.,Jaypee University of Information Technology
Current Topics in Medicinal Chemistry | Year: 2014

The activation of transcription factors nuclear factor-kappa B (NF-κB) and cyclooxygenase-2 (COX-2) is critical in cancer; they act synergistically in promoting tumor growth, survival, and resistance to chemotherapy. Thus, combined targeting of NF-κB and COX-2 present an opportunity for synergistic anticancer efficacy. The ester prodrugs of pentacyclic triterpenoids reduced lantadene A (3), B (4), and its congener 22β-hydroxyoleanonic acid (5) with various non steroidal anti-inflammatory drugs (NSAIDs) present a novel approach. The ester prodrugs of 3 and 4 with diclofenac showed promising dual inhibition of NF-κB and COX-2. The lead prodrugs 14 and 15 exhibited inhibition of inhibitor of nuclear factor-kappa B kinaseβ (IKKβ) in the single-digit micromolar range and at the same time, prodrugs 14 and 15 showed marked cytotoxicity against A549 lung cancer cell line with IC50s 0.15 and 0.42 μM, respectively. The prodrugs 14 and 15 exhibited stability in the acidic pH and were hydrolyzed readily in the human blood plasma to release the active parent moieties. Thus, we have synthesized novel hybrid compounds to target both NF-κB and COX-2 via a prodrug approach, leading to promising anticancer candidates. © 2014 Bentham Science Publishers.

Mohanty P.,National Research Center for Orchids | Das J.,Institute of Bioresources and Sustainable Development IBSD
Plant Growth Regulation | Year: 2013

Artificial seeds were obtained through encapsulation of protocorm-like bodies (PLBs) of Dendrobium densiflorum in calcium alginate beads. This paper demonstrates the alginate-encapsulation and conversion (complete plantlet regeneration) from PLBs, the effect of storage conditions (at different temperature; 4, 8, 16 °C, RT and duration; 15, 30, 45, 60, 75, 90 days) on viability of encapsulated plant materials as well as the assessment of genetic fidelity of the regenerants. Individual PLBs were encapsulated in calcium alginate beads for mass propagation, short-term storage and germplasm sharing. The superior gel matrix for encapsulation was obtained using 3 % sodium alginate and 100 mM calcium chloride (CaCl2·2H2O). The highest percentage of conversion (100 %) of encapsulated PLBs (capsules) was obtained on MS2 medium (MS medium + 2 mg/l BAP). Capsules were successfully stored till 60 days at 8 °C with conversion frequency of 95.5 %. Plantlets regenerated from encapsulated beads were acclimatized successfully with 95 % survival rate. A total of 40 primers were screened, out of which 10 primers successfully generated 39 scorable bands, ranging from 0.2 to 1.3 kb amplicons. The uniform RAPD banding profile among the plantlets derived from encapsulated PLBs following 60 days of storage confirmed genetic fidelity. © 2013 Springer Science+Business Media Dordrecht.

Aggarwal B.B.,University of Houston | Deb L.,University of Houston | Deb L.,Institute of Bioresources and Sustainable Development IBSD | Prasad S.,University of Houston
Molecules | Year: 2015

Curcumin (diferuloylmethane), a golden pigment from turmeric, has been linked with antioxidant, anti-inflammatory, anticancer, antiviral, antibacterial, and antidiabetic properties. Most of the these activities have been assigned to methoxy, hydroxyl, α,β-unsaturated carbonyl moiety or to diketone groups present in curcumin. One of the major metabolites of curcumin is tetrahydrocurcumin (THC), which lacks α,β-unsaturated carbonyl moiety and is white in color. Whether THC is superior to curcumin on a molecular level is unclear and thus is the focus of this review. Various studies suggest that curcumin is a more potent antioxidant than THC; curcumin (but not THC) can bind and inhibit numerous targets including DNA (cytosine-5)-methyltransferase-1, heme oxygenase-1, Nrf2, β-catenin, cyclooxygenase-2, NF-kappaB, inducible nitric oxide synthase, nitric oxide, amyloid plaques, reactive oxygen species, vascular endothelial growth factor, cyclin D1, glutathione, P300/CBP, 5-lipoxygenase, cytosolic phospholipase A2, prostaglandin E2, inhibitor of NF-kappaB kinase-1, -2, P38MAPK, p-Tau, tumor necrosis factor-α, forkhead box O3a, CRAC; curcumin can inhibit tumor cell growth and suppress cellular entry of viruses such as influenza A virus and hepatitis C virus much more effectively than THC; curcumin affects membrane mobility; and curcumin is also more effective than THC in suppressing phorbol-ester-induced tumor promotion. Other studies, however, suggest that THC is superior to curcumin for induction of GSH peroxidase, glutathione-S-transferase, NADPH: quinone reductase, and quenching of free radicals. Most studies have indicated that THC exhibits higher antioxidant activity, but curcumin exhibits both pro-oxidant and antioxidant properties.

Romi W.,Institute of Bioresources and Sustainable Development IBSD | Romi W.,Gauhati University | Keisam S.,Institute of Bioresources and Sustainable Development IBSD | Keisam S.,Gauhati University | And 2 more authors.
BMC Microbiology | Year: 2014

Background: Meyerozyma guilliermondii (anamorph Candida guilliermondii) and Meyerozyma caribbica (anamorph Candida fermentati) are closely related species of the genetically heterogenous M. guilliermondii complex. Conventional phenotypic methods frequently misidentify the species within this complex and also with other species of the Saccharomycotina CTG clade. Even the long-established sequencing of large subunit (LSU) rRNA gene remains ambiguous. We also faced similar problem during identification of yeast isolates of M. guilliermondii complex from indigenous bamboo shoot fermentation in North East India. There is a need for development of reliable and accurate identification methods for these closely related species because of their increasing importance as emerging infectious yeasts and associated biotechnological attributes. Results: We targeted the highly variable internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) and identified seven restriction enzymes through in silico analysis for differentiating M. guilliermondii from M. caribbica. Fifty five isolates of M. guilliermondii complex which could not be delineated into species-specific taxonomic ranks by API 20 C AUX and LSU rRNA gene D1/D2 sequencing were subjected to ITS-restriction fragment length polymorphism (ITS-RFLP) analysis. TaqI ITS-RFLP distinctly differentiated the isolates into M. guilliermondii (47 isolates) and M. caribbica (08 isolates) with reproducible species-specific patterns similar to the in silico prediction. The reliability of this method was validated by ITS1-5.8S-ITS2 sequencing, mitochondrial DNA RFLP and electrophoretic karyotyping. Conclusions: We herein described a reliable ITS-RFLP method for distinct differentiation of frequently misidentified M. guilliermondii from M. caribbica. Even though in silico analysis differentiated other closely related species of M. guilliermondii complex from the above two species, it is yet to be confirmed by in vitro analysis using reference strains. This method can be used as a reliable tool for rapid and accurate identification of closely related species of M. guilliermondii complex and for differentiating emerging infectious yeasts of the Saccharomycotina CTG clade. © 2014Romi et al.; licensee BioMed Central Ltd. ©2014 Romi et al.; licensee BioMed Central Ltd.

Jeyaram K.,Institute of Bioresources and Sustainable Development IBSD | Romi W.,Institute of Bioresources and Sustainable Development IBSD | Singh T.A.,Institute of Bioresources and Sustainable Development IBSD | Devi A.R.,Institute of Bioresources and Sustainable Development IBSD | Devi S.S.,Manipur University
International Journal of Food Microbiology | Year: 2010

Soidon is a non-salted acidic fermented food prepared from the succulent bamboo shoot tip of Schizostachyum capitatum Munro by using a traditional liquid starter called "soidon mahi" in Manipur state of India. In this study, 163 bacterial isolates associated with this starter samples were identified and their population distribution was investigated by amplified ribosomal DNA restriction analysis (ARDRA), 16S rDNA sequencing and randomly amplified polymorphic DNA (RAPD) analysis. This acidic starter (pH 4.5±0.15) was dominated by a characteristic association of Bacillus and lactic acid bacteria (LAB) together. The population distribution of dominant species were Bacillus subtilis 29.3%, Bacillus cereus 35.7%, Bacillus pumilus 2.6%, Lactobacillus brevis 9.6%, Lactobacillus plantarum 5.1%, Carnobacterium sp. 11.9%, Enterococcus faecium 1.2% and Pseudomonas fluorescens 4.6%. Alarming population load (106-107cfu/ml) of B. cereus in 87% of starter samples studied should raise concern regarding biosafety of soidon consumption. PCR amplification of 16S-23S rDNA intergenic transcribed spacer (ITS) region and ITS-RFLP profiles revealed a high diversity with eight subgroups in B. subtilis, five subgroups in B. cereus and three subgroups in L. brevis isolates. The most abundant B. subtilis subgroup IB.1 distributed in most of the samples showed very less clonal variability during RAPD analysis. The molecular methods used in this study identified the dominant strains of Bacillus and LAB distributed in most of the starter samples. These dominant strains of B. subtilis, L. brevis and L. plantarum would allow for developing a defined starter culture for the production of quality soidon. © 2010 Elsevier B.V.

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