Boor P.,RWTH Aachen |
Boor P.,Institute of Molecular Biomedicine |
Floege J.,RWTH Aachen
American Journal of Transplantation | Year: 2015
Renal tubulointerstitial fibrosis is the final common pathway of progressive renal diseases. In allografts, it is assessed with tubular atrophy as interstitial fibrosis/tubular atrophy (IF/TA). IF/TA occurs in about 40% of kidney allografts at 3-6 months after transplantation, increasing to 65% at 2 years. The origin of renal fibrosis in the allograft is complex and includes donor-related factors, in particular in case of expanded criteria donors, ischemia-reperfusion injury, immune-mediated damage, recurrence of underlying diseases, hypertensive damage, nephrotoxicity of immunosuppressants, recurrent graft infections, postrenal obstruction, etc. Based largely on studies in the non-transplant setting, there is a large body of literature on the role of different cell types, be it intrinsic to the kidney or bone marrow derived, in mediating renal fibrosis, and the number of mediator systems contributing to fibrotic changes is growing steadily. Here we review the most important cellular processes and mediators involved in the progress of renal fibrosis, with a focus on the allograft situation, and discuss some of the challenges in translating experimental insights into clinical trials, in particular fibrosis biomarkers or imaging modalities. The authors summarize key mechanisms of disease, therapeutic targets, and clinical issues in renal allograft fibrosis. © Copyright 2015 The American Society of Transplantation and the American Society of Transplant Surgeons.
Maria B.,General Military Hospital Ruzomberok |
Maria S.,Clinic of Infectology and Travel Medicine |
Roman G.,Institute of Molecular BioMedicine
Epidemiologie, Mikrobiologie, Imunologie | Year: 2012
Lyme borreliosis is a contemporary public health problem. That's why we devoted our prospective and retrospective epidemiological study to the incidence of anthropozoonosis in the regions of Liptovský Mikuláš and Ružomberok. We created a group of 476 patients from the medical documentation of infectious units. These patients were treated for Lyme borreliosis from 1989 to 2010, 280 patients in the county of Liptovský Mikuláš, and 196 patients in the county of Ružomberok. Morbidity in different years was compared to national data. The number of ill patients (n = 476) compared to data about reported cases from The Regional Public Health Office in Liptovský Mikuláš (n = 221) confirmed the lack of a warning service. The actual incidence of Lyme disease in the Liptov region is twice higher. The average morbidity was 16.24 people per 100,000 of the population for the last 22 years. The highest morbidity from 476 treated patients (men 37.52%, women 62.48%) was in the group aged from 45 to 54 years with an average annual morbidity 21.18/100,000. The study also confirmed the higher incidence of Lyme disease in the female population. © Česká lékařská společnost Jana Evangelisty Purkyně, Praha 2012.
Repiska G.,Institute of Molecular Biomedicine |
Repiska G.,Comenius University |
Sedlackova T.,Institute of Molecular Biomedicine |
Szemes T.,Comenius University |
And 2 more authors.
Fetal Diagnosis and Therapy | Year: 2015
Background: The accuracy and reliability of detection of free fetal DNA in plasma of pregnant women can be significantly improved by increasing the overall DNA concentration following the isolation from maternal plasma. The aim of our study was to compare DNA concentration methods on samples with free fetal DNA. Materials and Methods: DNA isolated from plasma samples of pregnant women carrying a male fetus were concentrated by 3 different methods: vacuum concentration, centrifugal filters and spin columns. Their performance was evaluated using PCR-based Y-chromosomal short tandem repeat (Y-STR) genotyping of the fetus. Results: A statistically significant difference was found between the 3 tested methods (F = 15.57, p < 0.0001). Using vacuum concentration 85.3% of paternally inherited Y-STR alleles were correctly identified. A significantly smaller proportion of alleles was correctly identified in samples concentrated by centrifugal filters and spin columns - 75.9 and 66.5%, respectively. Discussion: The highest proportion of paternally inherited Y-STR alleles was found in samples concentrated with the use of vacuum concentration. This concentration procedure does not require further handling of the sample either, which is an advantage because it avoids potential sample contamination. On the other hand, when automation is considered, vacuum concentration is less suitable because of an uneven and unpredictable sample evaporation rate. © 2014 S. Karger AG, Basel.