Institute of Biomedical Technology

Pátra, Greece

Institute of Biomedical Technology

Pátra, Greece
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Zippo A.G.,National Research Council Italy | Romanelli P.,Ab Medica | Martinez N.R.T.,A-D Technologies | Caramenti G.C.,Institute of Biomedical Technology | And 2 more authors.
Frontiers in Systems Neuroscience | Year: 2015

Artificial brain-machine interfaces (BMIs) represent a prospective step forward supporting or replacing faulty brain functions. So far, several obstacles, such as the energy supply, the portability and the biocompatibility, have been limiting their effective translation in advanced experimental or clinical applications. In this work, a novel 16 channel chronically implantable epicortical grid has been proposed. It provides wireless transmission of cortical recordings and stimulations, with induction current recharge. The grid has been chronically implanted in a non-human primate (Macaca fascicularis) and placed over the somato-motor cortex such that 13 electrodes recorded or stimulated the primary motor cortex and three the primary somatosensory cortex, in the deeply anaesthetized animal. Cortical sensory and motor recordings and stimulations have been performed within 3 months from the implant. In detail, by delivering motor cortex epicortical single spot stimulations (1–8 V, 1–10 Hz, 500 ms, biphasic waves), we analyzed the motor topographic precision, evidenced by tunable finger or arm movements of the anesthetized animal. The responses to light mechanical peripheral sensory stimuli (blocks of 100 stimuli, each single stimulus being <1 ms and interblock intervals of 1.5–4 s) have been analyzed. We found 150–250 ms delayed cortical responses from fast finger touches, often spread to nearby motor stations. We also evaluated the grid electrical stimulus interference with somatotopic natural tactile sensory processing showing no suppressing interference with sensory stimulus detection. In conclusion, we propose a chronically implantable epicortical grid which can accommodate most of current technological restrictions, representing an acceptable candidate for BMI experimental and clinical uses. © 2015 Zippo, Romanelli, Torres Martinez, Caramenti, Benabid and Biella.

Malataras P.G.,Institute of Biomedical Technology | Bliznakov Z.B.,University of Patras | Pallikarakis N.E.,University of Patras
IFMBE Proceedings | Year: 2015

The use of medical equipment management software tools has been introduced in the field of clinical engineering since the beginning of the 80’s. Nowadays the tremendous expansion of Internet has changed both the way these tools function and the capabilities they provide to the user. This work presents a medical equipment management software system which has been developed to operate as a web service. In addition, its main characteristics are presented in comparison with the previous client-server and standalone versions. Finally, the benefits of such an implementation are discussed. © Springer International Publishing Switzerland 2015.

Tsekouras K.,University of Patras | Papathanassopoulos F.,Institute of Biomedical Technology | Kounetas K.,University of Patras | Pappous G.,University of Patras
International Journal of Production Economics | Year: 2010

In the last decade, a significant amount of financial resources has been devoted by the Greek Government and the European Union to provide the intensive care units (ICU) of the Greek Public Health Care System with high-tech medical equipment in order to improve their productive efficiency. Using a unique data set, we employ the DEA bootstrap of Simar and Wilson (2007) approach to estimate the efficiency of each ICU and to explore the impact of these investments on their efficiency. Our results indicate that, although the technical efficiency is benefited from the embodiment of new medical technology, the scale efficiency remains unaffected. The role of the asymmetric information, of the ICUs' proximity to pools of knowledge and of the composition of the medical personnel, seems to be the crucial factors for the improvement of their productive efficiency. © 2010 Elsevier B.V.All rights reserved.

Staff S.,Laboratory of Cancer Biology | Staff S.,University of Tampere | Tolonen T.,Fimlab Laboratories | Laasanen S.-L.,University of Tampere | And 4 more authors.
International Journal of Gynecological Pathology | Year: 2014

Mutations in BRCA1 and BRCA2 genes confer an increased lifetime risk for breast and ovarian cancer. Increased lifetime ovarian cancer risk among BRCA1/BRCA2 mutation carriers can be substantially decreased by risk-reducing salpingo-oophorectomy (RRSO), which also provides material for molecular research on early pathogenesis of serous ovarian cancer. RRSO studies have suggested fallopian tube as a primary site of serous high-grade ovarian cancer. In this study, the nuclear expression levels of γ-H2AX and p53 using immunohistochemical (IHC) study was quantitatively assessed in ovarian and fallopian tube epithelium derived from RRSOs in 29 BRCA1 and BRCA2 mutation carriers and in 1 patient with a strong family history of breast and ovarian cancer but showing an unknown BRCA status. Both p53 and γ-H2AX nuclear staining levels were significantly higher in BRCA1/2 mutation-positive fallopian tube epithelium compared with the control fallopian tube epithelium (P<0.006 and P=0.011, respectively). Nuclear expression levels of p53 and γ-H2AX were similar between the BRCA1/2 mutation-positive ovarian epithelium and controls. Both γ-H2AX and p53 showed significantly higher nuclear expression levels in BRCA1/2 mutation-positive fallopian tube epithelium compared with BRCA1/2 mutation-positive ovarian epithelium (P<0.0001 and P<0.0001, respectively). BRCA1/2 mutation-positive fallopian tube epithelium showed a positive correlation between the γ-H2AX and p53 nuclear expression levels (Pearson r=0.508, P=0.003). Our results of quantitative nuclear p53 and γ-H2AX expression levels in ovarian and fallopian tube epithelium derived from RRSO in high-risk patients support the previously suggested role of fallopian tube epithelium serving as a possible site of initial serous ovarian carcinogenesis. © 2014 International Society of Gynecological Pathologists.

Parker B.C.,University of Houston | Annala M.J.,University of Houston | Annala M.J.,Tampere University of Technology | Cogdell D.E.,University of Houston | And 20 more authors.
Journal of Clinical Investigation | Year: 2013

Fusion genes are chromosomal aberrations that are found in many cancers and can be used as prognostic markers and drug targets in clinical practice. Fusions can lead to production of oncogenic fusion proteins or to enhanced expression of oncogenes. Several recent studies have reported that some fusion genes can escape microRNA regulation via 3'-untranslated region (3'-UTR) deletion. We performed whole transcriptome sequencing to identify fusion genes in glioma and discovered FGFR3-TACC3 fusions in 4 of 48 glioblastoma samples from patients both of mixed European and of Asian descent, but not in any of 43 low-grade glioma samples tested. The fusion, caused by tandem duplication on 4p16.3, led to the loss of the 3'-UTR of FGFR3, blocking gene regulation of miR-99a and enhancing expression of the fusion gene. The fusion gene was mutually exclusive with EGFR, PDGFR, or MET amplification. Using cultured glioblastoma cells and a mouse xenograft model, we found that fusion protein expression promoted cell proliferation and tumor progression, while WT FGFR3 protein was not tumorigenic, even under forced overexpression. These results demonstrated that the FGFR3-TACC3 gene fusion is expressed in human cancer and generates an oncogenic protein that promotes tumorigenesis in glioblastoma.

Teittinen K.J.,University of Tampere | Gronroos T.,University of Tampere | Parikka M.,Institute of Biomedical Technology | Ramet M.,Institute of Biomedical Technology | And 2 more authors.
Leukemia Research | Year: 2012

The zebrafish has proven to be a valuable vertebrate model in which to elucidate the molecular mechanisms of various diseases. A high degree of genetic and morphological similarity in hematopoiesis between the zebrafish and human indicates that zebrafish can provide valuable knowledge about the mechanisms behind pathogenesis of leukemia. To date, a small number of zebrafish leukemia models have been published and they have already provided some interesting information. However, the full potential of these models, especially the identification of contributing genetic factors and high-throughput drug screens, is yet to be fulfilled. Further transgenic or mutant animals are needed, especially for modeling high-risk leukemias, such as MLL rearranged infant leukemias. © 2012 Elsevier Ltd.

Pisu C.,Institute of Biomedical Technology | Pira L.,PharmaNess Scarl | Pani L.,Institute of Biomedical Technology
Behavioural Pharmacology | Year: 2010

There is growing evidence to show that atypical antipsychotic quetiapine might exert an anxiolytic effect in patients. Nevertheless, the mechanism underlying this effect has not yet been fully explored. Like other anxiolytic drugs, quetiapine exhibits partial agonistic activity toward serotonergic 1A (5HT1A) receptors. The involvement of the serotonin system in anxiety, particularly of 5HT1A receptors, has been widely documented. In this study we have investigated whether different doses of quetiapine (5, 10, and 30 mg/kg, oral gavage) administered to C57BL6/N mice could produce an anxiolytic effect in the Vogel conflict test, a classical model of anxiety, and whether or not the selective 5HT1A antagonist WAY100635 (0.1 mg/kg, subcutaneously) might prevent such an effect. Our results show that 10 mg/kg quetiapine exhibits an anxiolytic effect, that is, at least in part, 5HT 1A-mediated, because it is completely eliminated by WAY100635. © 2010 Wolters Kluwer Health | Lippincott Williams & Wilkins.

Sancini G.,University of Milan Bicocca | Farina F.,University of Milan Bicocca | Battaglia C.,University of Milan | Battaglia C.,Institute of Biomedical Technology | And 5 more authors.
PLoS ONE | Year: 2014

Oxidative stress, pulmonary and systemic inflammation, endothelial cell dysfunction, atherosclerosis and cardiac autonomic dysfunction have been linked to urban particulate matter exposure. The chemical composition of airborne pollutants in Milano is similar to those of other European cities though with a higher PM2.5 fraction. Milano winter fine particles (PM2.5win) are characterized by the presence of nitrate, organic carbon fraction, with high amount of polycyclic aromatic hydrocarbons and elements such as Pb, Al, Zn, V, Fe, Cr and others, with a negligible endotoxin presence. In BALB/c mice, we examined, at biochemical and transcriptomic levels, the adverse effects of repeated Milano PM2.5win exposure in lung and heart. We found that ET-1, Hsp70, Cyp1A1, Cyp1B1 and Hsp-70, HO-1, MPO respectively increased within lung and heart of PM2.5win-treated mice. The PM2.5win exposure had a strong impact on global gene expression of heart tissue (181 upregulated and 178 down-regulated genes) but a lesser impact on lung tissue (14 up-regulated genes and 43 downregulated genes). Focusing on modulated genes, in lung we found two- To three-fold changes of those genes related to polycyclic aromatic hydrocarbons exposure and calcium signalling. Within heart the most striking aspect is the twofold to threefold increase in collagen and laminin related genes as well as in genes involved in calcium signaling. The current study extends our previous findings, showing that repeated instillations of PM2.5win trigger systemic adverse effects. PM2.5win thus likely poses an acute threat primarily to susceptible people, such as the elderly and those with unrecognized coronary artery or structural heart disease. The study of genomic responses will improve understanding of disease mechanisms and enable future clinical testing of interventions against the toxic effects of air pollutant. © 2014 Sancini et al.

Celli J.,Leiden University | Dalgleish R.,University of Leicester | Vihinen M.,Institute of Biomedical Technology | Taschner P.E.,Leiden University | den Dunnen J.T.,Leiden University
Human Mutation | Year: 2012

Gene variant databases or Locus-Specific DataBases (LSDBs) are used to collect and display information on sequence variants on a gene-by-gene basis. Their most frequent use is in relation to DNA-based diagnostics, giving clinicians and scientists easy access to an up-to-date overview of all gene variants identified worldwide and whether they influence the function of the gene (" pathogenic or not"). While literature on gene variant databases is extensive, little has been published on the process of database curation itself. Based on our extensive experience as LSDB curators and our contributions to database curation courses, we discuss the subject of database curation. We describe the tasks involved, the steps to take, and the issues that might occur. Our overview is a first step toward establishing overall guidelines for database curation and ultimately covers one aspect of establishing quality-assured gene variant databases. © 2011 Wiley Periodicals, Inc.

Exarchos K.P.,University of Ioannina | Exarchos K.P.,Institute of Biomedical Technology | Exarchos T.P.,University of Ioannina | Exarchos T.P.,Institute of Biomedical Technology | And 6 more authors.
BMC Bioinformatics | Year: 2011

Background: In peptides and proteins, only a small percentile of peptide bonds adopts the cis configuration. Especially in the case of amide peptide bonds, the amount of cis conformations is quite limited thus hampering systematic studies, until recently. However, lately the emerging population of databases with more 3D structures of proteins has produced a considerable number of sequences containing non-proline cis formations (cis-nonPro).Results: In our work, we extract regular expression-type patterns that are descriptive of regions surrounding the cis-nonPro formations. For this purpose, three types of pattern discovery are performed: i) exact pattern discovery, ii) pattern discovery using a chemical equivalency set, and iii) pattern discovery using a structural equivalency set. Afterwards, using each pattern as predicate, we search the Eukaryotic Linear Motif (ELM) resource to identify potential functional implications of regions with cis-nonPro peptide bonds. The patterns extracted from each type of pattern discovery are further employed, in order to formulate a pattern-based classifier, which is used to discriminate between cis-nonPro and trans-nonPro formations.Conclusions: In terms of functional implications, we observe a significant association of cis-nonPro peptide bonds towards ligand/binding functionalities. As for the pattern-based classification scheme, the highest results were obtained using the structural equivalency set, which yielded 70% accuracy, 77% sensitivity and 63% specificity. © 2011 Exarchos et al; licensee BioMed Central Ltd.

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