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Moscow, United States

Medvedev A.E.,Institute of Biomedical Chemistry | Buneeva O.A.,Institute of Biomedical Chemistry | Kopylov A.T.,Institute of Biomedical Chemistry | Gnedenko O.V.,Institute of Biomedical Chemistry | And 5 more authors.
International Journal of Molecular Sciences | Year: 2015

The amyloid-β peptide is considered as a key player in the development and progression of Alzheimer’s disease (AD). Although good evidence exists that amyloid-β accumulates inside cells, intracellular brain amyloid-binding proteins remain poorly characterized. Proteomic profiling of rat brain homogenates, performed in this study, resulted in identification of 89 individual intracellular amyloid-binding proteins, and approximately 25% of them were proteins that we had previously identified as specifically binding to isatin, an endogenous neuroprotector molecule. A significant proportion of the amyloid-binding proteins (more than 30%) are differentially expressed or altered/oxidatively modified in AD patients. Incubation of brain homogenates with 70 μM hydrogen peroxide significantly influenced the profile of amyloid-β binding proteins and 0.1 mM isatin decreased the number of identified amyloid-β binding proteins both in control and hydrogen peroxide treated brain homogenates. The effects of hydrogen peroxide and isatin have been confirmed in optical biosensor experiments with purified glyceraldehyde-3-phosphate dehydrogenase, one of the known crucial amyloid-β binding proteins (also identified in this study). Data obtained suggest that isatin protects crucial intracellular protein targets against amyloid binding, and possibly favors intracellular degradation of this protein via preventing formation of amyloid-β oligomers described in the literature for some isatin derivatives. © 2014 by the authors; licensee MDPI, Basel, Switzerland.

Sanzhakov M.A.,Institute of Biomedical Chemistry | Ignatov D.V.,Institute of Biomedical Chemistry | Prozorovskii V.N.,Institute of Biomedical Chemistry | Druzhilovskaia O.S.,Institute of Biomedical Chemistry | And 2 more authors.
Biomedit͡sinskai͡a khimii͡a | Year: 2014

One of the main ways to increase the effectiveness of well-known medical formulations well-established in clinical medicine - development of delivery systems using new technological approaches and nanomaterials. Currently, much attention is given to targeted delivery systems. At the same time drug carrier has in addition to medication the so-called vector/address with a high affinity for binding to specific receptors on cells/tissue target. In this paper it is described the method for producing of address conjugates to over-expressed receptors on the tumor cells. As address fragment it was folic acid and as a linker was dodecylamine, causing inclusion the conjugate into lipid nanoparticles.Abstract available from the publisher.

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