Institute of Biology Bucharest of Romanian Academy

Bucharest, Romania

Institute of Biology Bucharest of Romanian Academy

Bucharest, Romania
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Stancu M.M.,Institute of Biology Bucharest of Romanian Academy
Brazilian Journal of Microbiology | Year: 2015

Recently, there has been a lot of interest in the utilization of rhodococci in the bioremediation of petroleum contaminated environments. This study investigates the response of Rhodococcus erythropolis IBBPo1 cells to 1% organic solvents (alkanes, aromatics). A combination of microbiology, biochemical, and molecular approaches were used to examine cell adaptation mechanisms likely to be pursued by this strain after 1% organic solvent exposure. R. erythropolis IBBPo1 was found to utilize 1% alkanes (cyclohexane, n-hexane, n-decane) and aromatics (toluene, styrene, ethylbenzene) as the sole carbon source. Modifications in cell viability, cell morphology, membrane permeability, lipid profile, carotenoid pigments profile and 16S rRNA gene were revealed in R. erythropolis IBBPo1 cells grown 1 and 24 h on minimal medium in the presence of 1% alkanes (cyclohexane, n-hexane, n-decane) and aromatics (toluene, styrene, ethylbenzene). Due to its environmental origin and its metabolic potential, R. erythropolis IBBPo1 is an excellent candidate for the bioremediation of soils contaminated with crude oils and other toxic compounds. Moreover, the carotenoid pigments produced by this nonpathogenic Gram-positive bacterium have a variety of other potential applications. © 2015, Sociedade Brasileira de Microbiologia.


Stancu M.M.,Institute of Biology Bucharest of Romanian Academy
Waste and Biomass Valorization | Year: 2017

The goal of this study was to isolate and characterize a new bacterium capable of tolerating high concentrations of toxic organic solvents. The production of several extracellular secondary metabolites by Pseudomonas aeruginosa IBBPo16 cells was investigated using a combination of cultural, biochemical and molecular methods. A gram-negative bacterium, P. aeruginosa strain IBBPo16 (GenBank KT315654) was isolated from Poeni oily sludge by enrichment cultures method. Based on 16S rRNA gene sequence, isolated bacterium exhibited 100% similarity with other P. aeruginosa strains from nucleotide database. P. aeruginosa IBBPo16 was able to tolerate 100% cyclohexane, n-hexane, n-decane, styrene, 40% ethylbenzene, and 5% toluene. Toluene and ethylbenzene were more toxic for this bacterium, as compared with cyclohexane, n-hexane, n-decane, and styrene. In the genomic DNA extracted from P. aeruginosa IBBPo16 cells alkane hydroxylase (alkB1), xylene monooxygenase (xylM), naphthalene dioxygenase (ndoM), hydrophobic/amphiphilic efflux 1 (HAE1), and rhamnosyltransferase 1 (rhlAB) genes were detected. P. aeruginosa IBBPo16 produced some extracellular secondary metabolites, such as rhamnolipid surfactant, pyocyanin, pyorubin and pyoverdin pigments, which are well recognized for their numerous potential applications. © 2017 Springer Science+Business Media Dordrecht


Manole A.,Institute of Biology Bucharest of Romanian Academy | Banciu C.,Institute of Biology Bucharest of Romanian Academy
Romanian Biotechnological Letters | Year: 2015

Ruscus aculeatus L. is an endangered plant species with medicinal and ornamental value, protected under the Habitat Directive. Micropropagation opens new directions for its ex situ conservation, as well as to generate large scale material for natural population reinforcement or for commercial purposes. In this respect we have established a long term in vitro culture to preserve ex situ its valuable germplasm and, as well as potential resource of plant material. To optimize the multiplication of the preserved material, various formulation of basal MS (Murashige and Skoog) culture medium were tested. From all tested combinations, a content of 5.37 μM NAA auxin (1-aphthylacetic acid) with 22.20 μM cytokinin BAP (6-benzylaminopurine) gave the best rate of shoot regeneration. The efficiency of the multiplication rate reached an average of 24 shoots per explants and culturing a single explant could produce more than 240 000 of shoots following 3 subcultures, within only 24 weeks. © 2015 University of Bucharest.


Stefanut S.,Institute of Biology Bucharest of Romanian Academy | Goia I.,Babes - Bolyai University
Nova Hedwigia | Year: 2012

This paper presents a checklist of the bryophytes of Romania and a Bryophyte Red List of Romania. 979 bryophyte species are recorded for Romania, of these, 4 are hornworts, 217 liverworts and 758 mosses. The conservation status of bryophytes was assessed in accordance with the IUCN guidelines 2011, version 9.0. The Red list includes 374 threatened species, 157 are "Critically Endangered", 113 "Endangered" and 104 "Vulnerable" species. © 2012 J. Cramer in Gebr. Borntraeger Verlagsbuchhandlung, Stuttgart, Germany.


Marilena Stancu M.,Institute of Biology Bucharest of Romanian Academy
Environmental Forensics | Year: 2015

Rhodococcus erythropolis IBBPo5 (KM405337) and Lysinibacillus xylanilyticus IBBPo7 (KM405338) showed good tolerance to 5% (v/v) organic solvents (alkanes, aromatics). However, 5% (v/v) alkanes (i.e., cyclohexane, n-hexane, n-decane) were less toxic for R. erythropolis IBBPo5 and L. xylanilyticus IBBPo7 cells, compared with 5% (v/v) aromatics (i.e., toluene, o-xylene, ethylbenzene). The high organic solvent tolerance of these two Gram-positive bacteria could be due to the presence in their genome of some catabolic (alkB1, todM, xylM) and trehalose-6-phosphate synthase (otsA1) genes. R. erythropolis IBBPo5 possesses three catabolic genes (i.e., alkB1, todM, xylM), while L. xylanilyticus IBBPo7 possesses only one catabolic gene (i.e., alkB1). Numerous adaptations involved in organic solvents tolerance were depicted in R. erythropolis IBBPo5 and L. xylanilyticus IBBPo7 cells exposed to 5% (v/v) alkanes and aromatics (i.e., changes in the cell growth rate, membrane permeability, cell morphology, biosurfactant emulsification index, carotenoids profile, DNA fingerprinting). Therefore, microbiological, biochemical, and DNA fingerprinting studies of the bacteria isolated from polluted environments could provide valuable information that may complement or supplement other forensic investigations. © 2015, Copyright © Taylor & Francis Group, LLC.


Stancu M.M.,Institute of Biology Bucharest of Romanian Academy
Journal of Environmental Sciences (China) | Year: 2014

A new Gram-positive bacterium, Rhodococcus erythropolis IBBPo1 (KF059972.1) was isolated from a crude oil-contaminated soil sample by enrichment culture method. R. erythropolis IBBPo1 was able to tolerate a wide range of toxic compounds, such as antibiotics (800-1000μg/mL), synthetic surfactants (50-200μg/mL), and organic solvents (40%-100%). R. erythropolis IBBPo1 showed good tolerance to both alkanes (cyclohexane, n-hexane, n-decane) and aromatics (toluene, styrene, ethylbenzene) with logPOW (logarithm of the partition coefficient of the solvent in octanol-water mixture) values between 2.64 and 5.98. However, alkanes were less toxic for R. erythropolis IBBPo1 cells, compared with aromatics. The high organic solvent tolerance of R. erythropolis IBBPo1 could be due to the presence in their large genome of some catabolic (alkB, alkB1, todC1, todM, xylM), transporter (HAE1) and trehalose-6-phosphate synthase (otsA1, KF059973.1) genes. Numerous and complex physiological cellular responses and adaptations involved in organic solvent tolerance were revealed in R. erythropolis IBBPo1 cells exposed 1 and 24hr to 1% organic solvents. R. erythropolis IBBPo1 cells adapt to 1% organic solvents by changing surface hydrophobicity, morphology and their metabolic fingerprinting. Considerable modifications in otsA1 gene sequence were also observed in cells exposed to organic solvents (except ethylbenzene). © 2014.


Mirancea N.,Institute of Biology Bucharest of Romanian Academy
Romanian Journal of Morphology and Embryology | Year: 2016

Here we review on the infrastructure, relationships and putative role of a new cell phenotype termed telocyte. Ultrastructural and immunochemical characteristics of telocytes, their identification in different tissues, homo- and heterocellular telocyte’s contacts and considerations concerning their putative role in normal as well as pathological conditions are largely presented. A special section of the paper is devoted to our opinion/hypothesis concerning the possibility that, to some extent, endoneurial fibroblast dendritic phenotype (existed in the peripheral nerve endoneurium) can be assimilated to the telocyte or, at least to the telocyte-like. In this respect, we report about a body of evidence that endoneurial fibroblasts dendritic cells share some infrastructural characteristics with telocyte phenotype. Telocytes involvement in pathology, tissue engineering and regenerative medicine is also debated. © 2016 Editura Academiei Romane. All rights reserved.


Stancu M.M.,Institute of Biology Bucharest of Romanian Academy
Water, Air, and Soil Pollution | Year: 2015

Shewanella putrefaciens IBBPo6 (KM405339) showed good tolerance to 5 % organic solvents. The growth was higher when S. putrefaciens IBBPo6 cells were exposed to n-decane, as compared with the growth of cells exposed to toluene, o-xylene, ethylbenzene, cyclohexane, or n-hexane. Thus, n-decane was less toxic for S. putrefaciens IBBPo6 cells, while toluene, o-xylene, ethylbenzene, cyclohexane, and n-hexane were more toxic for this bacterium. The release of nucleic acids was higher when S. putrefaciens IBBPo6 cells were exposed to toluene, o-xylene, ethylbenzene, cyclohexane, or n-hexane, as compared with the release of nucleic acids from control cells and n-decane exposed cells. The cell surface hydrophobicity increased when S. putrefaciens IBBPo6 cells were exposed to n-decane, while in the presence of toluene, o-xylene, ethylbenzene, cyclohexane, and n-hexane, a decrease in the cell surface hydrophobicity was acquired. The exposure of S. putrefaciens IBBPo6 cells to 5 % organic solvents had induced biofilms formation, and their structure differs according to the nature of the hydrophobic substrate. Two secondary metabolites (i.e., biosurfactants, carotenoids) were produced by S. putrefaciens IBBPo6 control cells, as well as by the cells exposed to 5 % organic solvents. S. putrefaciens IBBPo6 possesses alkB1 and alkM1 catabolic genes and HAE1 transporter gene. A homologue of otsA1 gene was also detected in this bacterium. Some differences between the polymerase chain reaction (PCR) patterns of S. putrefaciens IBBPo6 control cells and cells exposed to 5 % organic solvents were observed. Distinct repetitive sequence-based PCR (rep-PCR), random amplification of DNA fragments (RAPD), and amplified ribosomal DNA restriction analysis (ARDRA) patterns were also acquired in S. putrefaciens IBBPo6 cells exposed to 5 % organic solvents, compared with the control cells. © 2015 Springer International Publishing Switzerland.


Stancu M.M.,Institute of Biology Bucharest of Romanian Academy
Water, Air, and Soil Pollution | Year: 2012

Constanta harbor has been contaminated for decades with petroleum and petroleum products, which contain different toxic organic solvents. A novel solvent-tolerant bacterium, Vibrio alginolyticus IBBCt2 was isolated from a seawater sample (Constanta harbor). Alkanes (i.e., n-hexane, n-decane, cyclohexane) with logarithm of partition coefficient in n-octanol and water (log P OW )∈>∈3.35, were less toxic for V. alginolyticus strain IBBCt2, compared with aromatics (i.e., toluene, m-xylene, ethylbenzene) with log P OW∈ <∈3.17. The high organic solvent resistance of V. alginolyticus IBBCt2 could be due to the presence of some catabolic (alkB, alkB/alkB1, todC1, xylM, C23DO) and transporter (HAE1, acrAB) genes. The adaptation mechanisms, underlying cyclohexane, n-hexane, n-decane, toluene, m-xylene, and ethylbenzene resistance in V. alginolyticus IBBCt2 showed a complex response of cells 60 min after solvent shock (i.e., modifications of the cell viability, changes in the membrane's lipid and protein profile, modifications of the genomic fingerprinting). Exposure of V. alginolyticus IBBCt2 cells to salt stress decreases the organic solvents tolerance of this bacterium. © 2012 Springer Science+Business Media B.V.


Stancu M.M.,Institute of Biology Bucharest of Romanian Academy
Indian Journal of Biotechnology | Year: 2011

Alkanes (n-hexane, n-heptane) with logarithm of partition coefficient between n-octanol and water (log POW) 3.86 to 4.39 were less toxic to Aeromonas hydrophila IBBPo8 and Pseudomonas aeruginosa IBB Po10 as compared to aromatics (toluene, styrene, xylene isomers, ethylbenzene & propylbenzene) with log POW 2.64 to 3.69. The toxicity of 0.5% (v/v) second phase of organic solvents to these bacteria could be predictable on the basis of the solvents' log POW. The tolerance, viability, adhesion and β-galactosidase activity of A. hydrophila IBB Po8 and P. aeruginosa IBB Po10 cells in the presence of 0.5% (v/v) organic solvents varied significantly. The results indicated that A. hydrophila IBB Po8 was more susceptible to organic solvents than P. aeruginosa IBB Po10, whereas both the bacterial strains harbour plasmids. A. hydrophila IBB Po8 did not posses hydrophobe/amphiphile efflux 1 (HAE1) transporter genes, while P. aeruginosa IBB Po10 did posses these genes. The adaptation mechanisms (modification of cell hydrophobicity, induction of β-galactosidase activity and changes in the membrane's lipid and protein content) of bacterial cells, underlying solvent tolerance, in A. hydrophila IBB Po8 and P. aeruginosa IBB Po10 showed a complex response to the presence of 0.5% (v/v) organic solvents in the culture medium. Bacterial strains able to survive in the presence of organic solvents could be used in two-phase biotransformation systems with whole cells for adequate bioremediation of heavily contaminated sites and could be a source for new solvent-stable enzymes with different applications.

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