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Le Touquet – Paris-Plage, France

Delgehyr N.,Institute Of Biologie Of Lecole Normal Superieure Ibens | Delgehyr N.,French Institute of Health and Medical Research | Delgehyr N.,French National Center for Scientific Research | Meunier A.,Institute Of Biologie Of Lecole Normal Superieure Ibens | And 17 more authors.
Methods in Cell Biology | Year: 2015

Primary and motile cilia differ in their structure, composition, and function. In the brain, primary cilia are immotile signalling organelles present on neural stem cells and neurons. Multiple motile cilia are found on the surface of ependymal cells in all brain ventricles, where they contribute to the flow of cerebrospinal fluid. During development, monociliated ependymal progenitor cells differentiate into multiciliated ependymal cells, thus providing a simple system for studying the transition between these two stages. In this chapter, we provide protocols for immunofluorescence staining of developing ependymal cells in vivo, on whole mounts of lateral ventricle walls, and in vitro, on cultured ependymal cells. We also provide a list of markers we currently use to stain both types of cilia, including proteins at the ciliary membrane and tubulin posttranslational modifications of the axoneme. © 2015 Elsevier Inc.

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