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Nantes, France

Denis M.-G.,Institute Of Biologie
Revue de Pneumologie Clinique | Year: 2011

Screening of EGFR mutations in the 28 molecular biology centers that responded to a questionnaire obtained a mutation rate of 13% during the first semester of 2010. 87% of tested tumors were adenocarcinomas, but 79% of the centers test also other histological types. The main EGFR mutations sites (exons 19 and 21) were tested in all the centers. The most used technique is sequencing, whereas it is considered as the low sensitive test by most of the centers. Therefore many platforms are in the process of improving their technique and for some of them to choose alternative targeted techniques, with also stronger intra- and inter-center quality controls. © 2011 Elsevier Masson SAS.

Victorri-Vigneau C.,University of Nantes | Victorri-Vigneau C.,Institute Of Biologie | Feuillet F.,University of Nantes | Wainstein L.,University of Nantes | And 3 more authors.
European Journal of Clinical Pharmacology | Year: 2013

Purpose: Zolpidem and zopiclone are two widely used non-benzodiazepine hypnotics whose usage seems to be associated to pharmacodependence. However, to our knowledge, there has as yet been no published epidemiological study which has compared their abuse or dependence potential. We used a pharmacoepidemiological approach to identify and characterise zolpidem and zopiclone users in real life situations. Methods: Regular users of zolpidem or zopiclone were identified in the database of a French regional health insurance organisation. A latent class analysis (LCA) was used to identify different subgroups of users of these two hypnotics. Results: The study cohort comprised 25,168 patients who regularly used zolpidem and 21,860 who regularly used zopiclone. The results of the latent class analysis, which enables subgroups with similar patterns of response to be identified, revealed four clinical subtypes of users of zolpidem: non-problematic users, users with associations with hypnotics/anxiolytics or with associated mental disorders, and problematic users. Only three subgroups were identified for zopiclone, and LCA did not discriminate a special class of problematic users for this drug. Conclusion: Our analysis indicates that there is a subclass of zolpidem user suggestive of abuse; this was not the case for zopiclone. This methodology is very interesting because it allows analysis of databases and determination of a specific signature of drugs potentially leading to abuse or dependence. © 2013 Springer-Verlag Berlin Heidelberg.

Munshi N.C.,Harvard University | Munshi N.C.,Dana-Farber Cancer Institute | Avet-Loiseau H.,University of Nantes | Avet-Loiseau H.,French Institute of Health and Medical Research | Avet-Loiseau H.,Institute Of Biologie
Clinical Cancer Research | Year: 2011

Multiple myeloma (MM) is a complex disease that is driven by numerous genetic and epigenetic alterations. Comprehensive oncogenomic analysis indicates the presence of many highly recurrent and highly focal amplifications and/or deletions in the MM genome. Integrated oncogenomic analyses of human MM have identified candidates resident within regions of amplification and/or deletions that are predicted to be involved in MM pathogenesis and progression. The biological behavior and clinical outcome in MM are dependent on these molecular determinants, which are also attractive therapeutic targets. The data obtained from extensive analysis of patient samples, with annotated clinical outcomes, are providing insights into molecular mechanisms of disease behavior, helping to develop sensitive prognostic models, identifying novel therapeutic targets, providing the framework for the development of molecularly based therapies, and, eventually, will help in developing individualized therapy to improve outcomes, with reduced toxicity. ©2011 AACR.

Barrett R.D.H.,University of British Columbia | Paccard A.,Institute Of Biologie | Healy T.M.,University of British Columbia | Bergek S.,Institute of Coastal Research | And 3 more authors.
Proceedings of the Royal Society B: Biological Sciences | Year: 2011

Climate change is predicted to lead to increased average temperatures and greater intensity and frequency of high and low temperature extremes, but the evolutionary consequences for biological communities are not well understood. Studies of adaptive evolution of temperature tolerance have typically involved correlative analyses of natural populations or artificial selection experiments in the laboratory. Field experiments are required to provide estimates of the timing and strength of natural selection, enhance understanding of the genetics of adaptation and yield insights into the mechanisms driving evolutionary change. Here, we report the experimental evolution of cold tolerance in natural populations of threespine stickleback fish (Gasterosteus aculeatus). We show that freshwater sticklebacks are able to tolerate lower minimum temperatures than marine sticklebacks and that this difference is heritable. We transplanted marine sticklebacks to freshwater ponds and measured the rate of evolution after three generations in this environment. Cold tolerance evolved at a rate of 0.63 haldanes to a value 2.5°C lower than that of the ancestral population, matching values found in wild freshwater populations. Our results suggest that cold tolerance is under strong selection and that marine sticklebacks carry sufficient genetic variation to adapt to changes in temperature over remarkably short time scales. © 2011 The Royal Society.

Villarreal-Silva M.,National Polytechnic Institute of Mexico | Suarez-Sanchez R.,National Polytechnic Institute of Mexico | Rodriguez-Munoz R.,National Polytechnic Institute of Mexico | Mornet D.,Institute Of Biologie | Cisneros B.,National Polytechnic Institute of Mexico
Neurochemical Research | Year: 2010

We have adopted the PC12 cell line as in vitro cell model for studying Dp71 function in neuronal cells. These cells express a cytoplasmic (Dp71f) and a nuclear (Dp71d) isoform of Dp71 as well as various dystrophin-associated proteins (DAPs). In this study, we revealed by confocal microscopy analysis and Western blotting evaluation of cell fractions the presence of different DAPs (β-dystroglycan, β-dystrobrevin, ε-sarcoglycan and γ1-syntrophin) in the nucleus of PC12 cells. Furthermore, we established by immunoprecipitation assays that Dp71d and the DAPs form a dystrophin-associated protein complex (DAPC) in the nucleus. Interestingly, depletion of Dp71 by antisense treatment (antisense-Dp71 cells) provoked a drastic reduction of nuclear DAPs, which indicates that Dp71d is critical for DAPs stability within the nucleus. Although Up71, the utrophin gene product homologous to Dp71, exhibited increased expression in the antisense-Dp71 cells, its scarce nuclear levels makes unlikely that could compensate for Dp71 nuclear deficiency. © 2009 Springer Science+Business Media, LLC.

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