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Singh A.,N 8 | Singh U.P.,N 8 | Srivastava R.,Institute of Bioengineering and Biological science
Archives of Phytopathology and Plant Protection | Year: 2010

Mango (Mangifera indica L.) suffers from floral and vegetative malformation and crop production is seriously affected. The anti-fungal activity of ethanolic extract of malformed mango inflorescence was observed at different concentrations (1000, 2000, 3000, 4000 and 5000 ml/ml) against 10 fungi, viz., Ustilago cynodontis, Cercospora cajani, Sphaerotheca sp., Cercospora sp., Alternaria solani, Bipolaris sp., Helminthosporium sp., Curvularia sp., Fusarium udum and Alternaria cajani. Spore germination of most of the fungi was inhibited at 5000 mg/ml. Some of them were also susceptible at 3000 or 4000 mg/ml concentration. Analysis of phenolic acids by high performance liquid chromatography (HPLC) showed 18 peaks in the extract, but only four could be identified, viz., capachin, gallic, benzoic and cinnamic acids. Because of the high efficacy of ethanolic extract of malformed mango inflorescence, its use under field conditions to control some plant diseases has been suggested. © 2010 Taylor & Francis. Source


Singh U.P.,N 8 | Gohain L.,Banaras Hindu University | Singh A.,Institute of Bioengineering and Biological science | Maurya S.,Banaras Hindu University | Sahni S.,Banaras Hindu University
Archives of Phytopathology and Plant Protection | Year: 2010

High performance liquid chromatographic (HPLC) analysis of mycelia of Sclerotium rolfsii grown on neem cake, and Zephyarenthes citrina bulb incorporated media was carried out. Several phenoloic acids, e.g., gallic, tannic, caffeic, cinnamic, chlorogenic and O-coumeric acids, were found in considerable amounts in treated mycelial mat as compared to the control. The amount of phenloic acids increased with increased concentration of both the materials in mycelia of 7 and 14 day-old cultures. Due to anti-oxidant and several other properties of phenolic acids, the senescence of the fungus has been prolonged which may be one probable reason of sustaining the virulence of the pathogen. © 2010 Taylor & Francis. Source


Sriivastava R.,Institute of Bioengineering and Biological science | Shalini,Institute of Bioengineering and Biological science
Internet Journal of Microbiology | Year: 2010

Antifungal activity of different strains of Pseudomonas fluorescens were tested against some plant pathogens such as Alternaria cajani, Curvularia lunata, Fusarium sp., Bipolaris sp. and Helminthosporium sp. in in vitro. Different concentrations (1000, 2000, 3000, 4000 and 5000 μg/mL) of Pseudomonas fluorescens were used and maximum spore germination of fungus was inhibited at 4000 and 5000 μg/mL. The result indicated that all the strains of Pseudomonas fluorescens presented a most significant value against Alternaria cajani and Curvularia lunata. Out of the five strains studied, the best result was shown by A-5, which showed almost complete inhibition against pathogenic fungi such as Curvularialunata and Fusarium sp. at 4000 and 5000 μg/mL while strain L-5 was resistant against Fusarium sp. and Helminthosporium sp. at 5000 μg/mL. Among the fungus tested, bacterial strains C-03 and Pf4-1 were found to be more sensitive to Fusarium sp. and Helminthosporium sp. Copyright Internet Scientific Publications, LLC., 1996 to 2010. Source


Prasad D.,Govind Ballabh Pant University of Agriculture & Technology | Singh A.,Institute of Bioengineering and Biological science | Singh K.P.,Govind Ballabh Pant University of Agriculture & Technology | Bist S.,Hemwati Nandan Bahuguna Garhwal University | And 2 more authors.
Archives of Phytopathology and Plant Protection | Year: 2010

The present study was conducted in the hill station of G B Pant University of Agriculture and Technology, Ranichauri, Uttarakhand, which is known for its diversity in medicinal and aromatic plants. The plant geranium, also known as rose or lemon geranium is highly valued for its essential oil which is used in the cosmetic and preferring industries. The plant suffers due to stem and root rot both during summer and winter seasons. In the present study, geranium plants showed maximum stem rot incidence (60.55%) and plant mortality (17.20%) during the winter season, whereas in summer the crops showed maximum root rot and wilt complex incidence (38.33%) with plant mortality up to (12.22%). The average stem rot mortality was comparatively low (16-20%) in summer but increased to 18-26% in winter crop. Several phenolic acids such as as tannic, gallic, caffeic, ferulic and benzoic were detected by using high performance liquid chromatography (HPLC) in variable amounts in these plants. The disease intensity varied from 97.77 to 92.46% from season to season. The significance of the phenolic compounds is discussed in relation to disease prevalence. © 2010 Taylor & Francis. Source


Singh U.P.,Institute of Bioengineering and Biological science | Maurya S.,Banaras Hindu University | Singh A.,Institute of Bioengineering and Biological science | Singh M.,Banaras Hindu University
Archives of Phytopathology and Plant Protection | Year: 2010

Neem (Azardirachta indica) is regarded as 'village pharmacy' in India due to its enormous use in the Indian villages as a folk medicine. Its different parts have been explored scientifically, except 'Neem toddy', which is a white secretion that exudes from the angle formed between the two major branches of old neem trees. The exact age of the tree upon the secretion of this white fluid is not known, nor why such a secretion occurs. It is normal, though rare, for such secretions to occur in old trees. The taste of neem toddy is bitter. The white milky liquid secretion, the 'neem toddy', was collected from the tree and assayed for the presence of phenolic acids by high performance liquid chromatography (HPLC). The phenolic acids detected were caffeic, vanillic, o-coumaric, cinnamic and salicylic acids. It was further fractionated by ethyl acetate and this fraction was tested against some fungi for antifungal activity. It was observed to be highly antifungal in vitro. Field experiments also showed its high efficacy against balsam (Impatiens balsamina) powdery mildew (Erysiphe cichoracearum). © 2010 Taylor & Francis. Source

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