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Fonvielle M.,University Pierre and Marie Curie | Fonvielle M.,University of Paris Descartes | Li De La Sierra-Gallay I.,Institute Of Biochimie Et Of Biophysique Moleculaire Et Cellulaire | Li De La Sierra-Gallay I.,French National Center for Scientific Research | And 20 more authors.
Angewandte Chemie - International Edition | Year: 2013

To gain insight into the catalytic mechanism of non-ribosomal amino acid transferases, peptidyl-RNA conjugates were synthesized for co-crystallization with FemXWv of Weissella viridescens, which transfers L-Ala from Ala-tRNAAla to the peptidoglycan precursor UDP-MurNAc-pentapeptide. The structure of the resulting complex and mutational studies revealed the mechanism by which FemXWv binds its substrates for substrate-assisted catalysis and stabilization of the tetrahedral intermediate. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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