Institute Of Bacteriologie

Strasbourg, France

Institute Of Bacteriologie

Strasbourg, France
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Sina H.,University Abomey Calavi | Ahoyo T.A.,University Abomey Calavi | Moussaoui W.,Institute Of Bacteriologie | Keller D.,Institute Of Bacteriologie | And 6 more authors.
BMC Microbiology | Year: 2013

Background: Staphylococcus aureus is an opportunistic commensal bacterium that mostly colonizes the skin and soft tissues. The pathogenicity of S. aureus is due to both its ability to resist antibiotics, and the production of toxins. Here, we characterize a group of genes responsible for toxin production and antibiotic resistance of S. aureus strains isolated from skin, soft tissue, and bone related infections. Results: A total of 136 S. aureus strains were collected from five different types of infection: furuncles, pyomyositis, abscesses, Buruli ulcers, and osteomyelitis, from hospital admissions and out-patients in Benin. All strains were resistant to benzyl penicillin, while 25% were resistant to methicillin, and all showed sensitivity to vancomycin. Panton-Valentine leukocidin (PVL) was the most commonly produced virulence factor (70%), followed by staphylococcal enterotoxin B (44%). Exfoliative toxin B was produced by 1.3% of the strains, and was only found in isolates from Buruli ulcers. The tsst-1, sec, and seh genes were rarely detected (≤1%). Conclusions: This study provides new insight into the prevalence of toxin and antibiotic resistance genes in S. aureus strains responsible for skin, soft tissue, and bone infections. Our results showed that PVL was strongly associated with pyomyositis and osteomyelitis, and that there is a high prevalence of PVL-MRSA skin infections in Benin. © 2013 Sina et al.; licensee BioMed Central Ltd.

Sina H.,University Abomey Calavi | Baba-Moussa F.,University Abomey Calavi | Ahoyo T.A.,University Abomey Calavi | Mousse W.,University Abomey Calavi | And 5 more authors.
African Journal of Microbiology Research | Year: 2011

A wide range of clinical samples were screened for identification of Staphylococcus aureus, their antibiotic sensitivity profile and the production of different leucotoxin and epidermolysins was evaluated. Out of 2,040 biological samples (collected from pus, urine, sperms, genital, catheter and blood of hospitalized and extra-hospital patients) screened, 123 pure cultures of S. aureus colonies were isolated. 48.78% of S. aureus were resistant to methicillin (MRSA), while 78% of them were isolated from extra-hospital patients. The S. aureus isolated from urines, pus and blood produced Panton and Valentine leukocidin (PVL) toxin, while the leucotoxin lukE-lukD was exclusively encountered by S. aureus isolated from pus samples. None of the bacterial colony isolated produced epidermolysin toxins A and B. In addition, 3.25% of MRSA and 8.13% of methicillin sensitive S. aureus (MSSA) produced PVL respectively. Our results indicated high frequency rate of MRSA in extra-hospital screened samples isolated from various types of infection. This high resistance rate combined with toxin production increases the virulence of S. aureus colonies and put therefore at risk the life of the patients in developing countries where auto-medication is not controlled. There is the need to instruct the population in order to avoid further widening of MRSA territory. © 2011 Academic Journals.

Baba-Moussa L.,University Abomey Calavi | Sina H.,University Abomey Calavi | Scheftel J.-M.,Institute Of Bacteriologie | Moreau B.,Center Hospitalier Of Cayenne | And 4 more authors.
PLoS ONE | Year: 2011

Panton-Valentine Leucocidin (PVL), one of the β-barrel pore-forming staphylococcal leucotoxins, is known to be associated to furuncles and some severe community pneumonia. However, it is still uncertain how many other virulence factors are also associated to furuncles and what the risk factors of furuncles are in immuno-compromised status of patients, especially the HIV (+) patients. In this paper, we use antigen immunoprecipitation and multiplex PCR approach to determine the presence of 19 toxins, 8 adhesion factors and the PFGE profiles associated to furuncles in three independent patient study groups of S. aureus (SA) isolates collected from the Cayenne General Hospital (French Guiana). The patient groups were made of: 16 isolates from HIV (-) patients, 9 from HIV (+) patients suffering from furuncles, and 30 control isolates from patients with diverse secondary infected dermatitis. Our data reveals that the majority (96%) of SA strains isolated from HIV patient-derived furuncles significantly produced PVL (p&10 -7), whereas only 10% of SA strains produced this toxin in secondary infected dermatosis. A high prevalence of LukE-LukD-producing isolates (56 to 78%) was recorded in patient groups. Genes encoding clumping factor B, collagen- and laminin-binding proteins (clfB, cna, lbp, respectively) were markedly frequent (30 to 55%), without being associated to a specific group. Pulse field gel electrophoresis evidenced 24 overall pulsotypes, whereas the 25 PVL-producing isolates were distributed into 15 non clonal fingerprints. These pulsotypes were not specific PVL-producing isolates. PVL appears to be the major virulence factor associated to furuncles in Europe and in South America regardless of the immune status of the HIV patients. © 2011 Baba-Moussa et al.

Claudon P.,CNRS Strasbourg Institute of Chemistry | Violette A.,CNRS Strasbourg Institute of Chemistry | Lamour K.,CNRS Strasbourg Institute of Chemistry | Decossas M.,CNRS Strasbourg Institute of Chemistry | And 11 more authors.
Angewandte Chemie - International Edition | Year: 2010

"Chemical Equation Presented" Fraternal twins: Oligoureas and Y-peptides are isosteric, quasi-isostructural helical foldamers endowed with distinct blomolecular recognition properties. Combination of the two backbones to generate urea/amide hybrids (see picture) was found to give more potent yet less cytotoxic antimicrobial helical foldamers. © 2010 Wiley-VCH Vertag GmbH &. Co. KGaA.

Aslam R.,University of Strasbourg | Laventie B.-J.,Institute Of Bacteriologie | Laventie B.-J.,University of Basel | Marban C.,University of Strasbourg | And 7 more authors.
Journal of Proteome Research | Year: 2013

Staphylococcus aureus is responsible for severe bacterial infections in hospitals and healthcare facilities. It produces single and bicomponent toxins (leukotoxins and hemolysins) that hinder innate immune function. Leukotoxin subunits bind to leukocyte cell membrane thus inducing transmembrane pores and subsequently, cell lysis. Leukotoxin LukE/D is a member of the bicomponent toxin family, but to date, no study concerning its involvement in host-pathogen interactions has been reported. In the present study, we performed the proteomic analysis of the secretions recovered after activation of human neutrophils by leukotoxin LukE/D. The neutrophil secretions were purified by RP-HPLC and different fractions were analyzed by Edman sequencing, LC-MS/MS, immunoblotted for chromogranin-derived peptides and further analyzed for antimicrobial properties. Proteomic analysis revealed that neutrophil secretions constitute a large number of proteins related with immune boosting mechanisms, proteolytic degradation, inflammatory process and antioxidant reactions. © 2013 American Chemical Society.

Alessandrini A.,CNR Institute of Neuroscience | Alessandrini A.,University of Modena and Reggio Emilia | Viero G.,CNR Institute of Biophysics | Dalla Serra M.,CNR Institute of Biophysics | And 2 more authors.
Biochimica et Biophysica Acta - Biomembranes | Year: 2013

γ-Hemolysins are bicomponent β-barrel pore forming toxins produced by Staphylococcus aureus as water-soluble monomers, which assemble into oligomeric pores on the surface of lipid bilayers. Here, after investigating the oligomeric structure of γ-hemolysins on supported lipid bilayers (SLBs) by atomic force microscopy (AFM), we studied the effect produced by this toxin on the structure of SLBs. We found that oligomeric structures with different number of monomers can assemble on the lipid bilayer being the octameric form the stablest one. Moreover, in this membrane model we found that γ-hemolysins can form clusters of oligomers inducing a curvature in the lipid bilayer, which could probably enhance the aggressiveness of these toxins at high concentrations. © 2012 Elsevier B.V. All rights reserved.

Gaucher D.,Hopitaux Universitaires Of Strasbourg | Gaucher D.,Institute Of Bacteriologie | Chartier C.,Novartis | Cohen S.-Y.,Center Ophtalmologique Dimagerie Et Of Laser | And 5 more authors.
Journal Francais d'Ophtalmologie | Year: 2016

High myopia (HM), which affects 0.9 to 3.1% of the population,is a major cause of vision loss. The purpose of this investigation was to study and evaluatethe impact of their high myopia on the daily lives of patients and to better understand theirexpectations in order to better meet their needs.Materials and methods.-The survey was conducted between February 19 and March 21, 2014.To be eligible, patients had to have myopia of at least-8 diopters and be over 40 years of age,with or without myopic complications. Patients' degree of myopia was defined as the opticalprescription of their worse eye. The 123 patients included were interviewed by phone usinga questionnaire developed and validated by a scientific committee composed of experts andmembers of the patients' Association against myopic maculopathy (AMAM). The phone interview,semi-structured, lasted 20 minutes.Results.-On average, myopia was-11.7 diopters. Women accounted for 71% of the population,89% of patients were under 65 years and were mostly professionals (65%). Over half of thesubjects reported myopic complications; 5% of patients had choroidal neovascularization (CNV).Only 29% had been informed of the risk of CNV or maculopathy. HM was a handicap in sportsfor 64% of patients, in leisure activities for 51%, and in professional activities for more than aquarter (28%). Only 56% of HM patients reported living perfectly well with their condition.Conclusions.-This survey is the first study on the daily life of people with HM aiming to analyzetheir social and emotional environment. It shows that HM has a profound impact on the daily livesof patients and may affect social life and professional activity. Most myopic patients possessincomplete or unclear information about the nature and risk of myopic complications. Theyexpressed the desire to be better informed about their condition earlier, before the onset ofcomplications. © 2015 Elsevier Masson SAS. All rights reserved.

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