Institute of Aquaculture of Torre la Sal IATS CSIC

Aldeadávila de la Ribera, Spain

Institute of Aquaculture of Torre la Sal IATS CSIC

Aldeadávila de la Ribera, Spain
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Nacher-Mestre J.,Jaume I University | Nacher-Mestre J.,Institute of Aquaculture of Torre la Sal IATS CSIC | Nacher-Mestre J.,Institute of Aquaculture Torre la Sal | Ibanez M.,Jaume I University | And 8 more authors.
Chemosphere | Year: 2016

There is an on-going trend for developing more sustainable salmon feed in which traditionally applied marine feed ingredients are replaced with alternatives. Processed animal products (PAPs) have been re-authorized as novel high quality protein ingredients in 2013. These PAPs may harbor undesirable substances such as pharmaceuticals and metabolites which are not previously associated with salmon farming, but might cause a potential risk for feed and food safety. To control these contaminants, an analytical strategy based on a generic extraction followed by ultra-high performance liquid chromatography coupled to high resolution mass spectrometry (UHPLC-HRMS) using quadrupole time-of-flight mass analyzer (QTOF MS) was applied for wide scope screening. Quality control samples, consisting of PAP commodities spiked at 0.02, 0.1 and 0.2 mg/kg with 150 analytes, were injected in every sample batch to verify the overall method performance. The methodology was applied to 19 commercially available PAP samples from six different types of matrices from the EU animal rendering industry. This strategy allows assessing possible emergent risk exposition of the salmon farming industry to 1005 undesirables, including pharmaceuticals, several dyes and relevant metabolites. © 2016 Elsevier Ltd.


Rasines I.,Spanish Oceanographic Institute | Gomez M.,Spanish Oceanographic Institute | Martin I.,Spanish Oceanographic Institute | Rodriguez C.,Spanish Oceanographic Institute | And 2 more authors.
Aquaculture | Year: 2012

One method to overcome the difficulties involved in reproducing first generation Senegalese sole that are born in captivity (F1) is to obtain gametes by stripping and then using them for artificial fertilization. The aim of this paper was to determine an appropriate protocol for hormonal induction of ovulation and artificial fertilization. To induce ovulation, two different GnRHa administration methods, weekly injections versus a single GnRHa implant, were compared. The number of spawns per female and the relative fecundity were significantly higher in females that received the hormonal therapy through repeated injection (6.3±0.8 spawns per female and 574.9±67.2×10 3eggskg -1 BW) than those treated with a single implant (2.7±0.6 spawns per female and 134.6±40.9×10 3eggskg -1 BW). The lapsed time to ovulation and the changes in fertilization and hatching rates of eggs that were retained in the ovarian cavity after ovulation were determined after GnRHa treatment. At 16°C, ovulation was detected at 41-44h post-treatment in 70% of the females. The highest fertilization and hatching rates were obtained during the first 3h after ovulation, and they significantly decreased thereafter. The results suggested that the GnRHa injection was the most appropriate administration method for the induction of ovulation. Ovulated eggs inside the female were viable for a short time, and therefore it is important to determine the time of ovulation after hormonal treatment. © 2011 .


Rasines I.,Spanish Oceanographic Institute | Gomez M.,Spanish Oceanographic Institute | Martin I.,Spanish Oceanographic Institute | Rodriguez C.,Spanish Oceanographic Institute | And 2 more authors.
Aquaculture | Year: 2013

To develop a protocol for the hormonal induction of ovulation and artificial fertilisation in Senegalese sole, it is necessary to determine whether the time of day of the GnRHa injection influences the lapsed time to ovulation or the egg quality. Hormonal treatments were administered at 06:00, 12:00 and 19:00. h. The time of ovulation was 42.5. ±0.6. h following hormonal treatment, and there were no significant differences between groups. However, it was noted that the time of ovulation was the most homogeneous in Groups 6 and 12, and most females in these groups ovulated at 41 or 44. h following hormonal treatment. Fertilisation rates were lower in fish injected at 19:00. h than in those injected at 06:00 or 12:00. h, and the largest number of larvae per kg of each female was obtained when the treatment was administered at 06:00. These results suggest that hormonal treatments should be applied in the early morning to promote the maximum number of good quality eggs. © 2013.


Oliveira C.,University of Murcia | Duncan N.J.,IRTA - Institute of Agricultural-Alimentary Research and Technology | Pousao-Ferreira P.,IPIMAR | Mananos E.,Institute of Aquaculture of Torre La Sal IATS CSIC | Sanchez-Vazquez F.J.,University of Murcia
Aquaculture | Year: 2010

The effect of the moon light cycle on plasma melatonin rhythms was examined in Senegal sole (Solea senegalensis) exposed to natural outdoor or artificial indoor lighting conditions. Furthermore, in a second experiment, the effect of the lunar cycle on vitellogenin and sex steroids (Testosterone, T; Estradiol, E2; 11-ketotestosterone, 11kt) was studied using mature individuals during reproductive season. In the first experiment, during full moon, plasma melatonin peaked at night in covered tanks (deprived of night illumination) from both outdoor (133.2±12.8pgml-1) and indoor (190.6±41.5pgml-1) groups. However, for fish in the open tanks, exposed to approximately 0.3lx of illumination, nocturnal plasma melatonin was significantly reduced (p<0.05), approaching to mid-light (ML) values, (79.6±7.1 and 81.8±14.0pgml-1, for outdoor and indoor groups, respectively). During new moon a similar pattern was observed in outdoor group: fish in the covered tank showed higher melatonin values than those in the open tank, which were exposed to the near undetectable night illumination. In the second experiment, plasma sex steroid concentrations were significantly higher during the full moon compared to the new moon. In the case of females, E2 concentration reduced from 2.4±0.6 to 0.4±0.1ngml-1 between full and new moon samplings, while T decreased from 0.3±0.0 to 0.2±0.0ngml-1. Vitellogenin, however, did not show such differences between moon phases. In males, 11kt exhibited a plasma concentration of 14.3±2.1ngml-1 during full moon and 4.7±0.7ngml-1 during new moon while T values were 2.6±0.4 and 1.0±0.1ngml-1 for full and new moon, respectively. In conclusion, these findings pointed out the high sensitivity to moon light of the Senegal sole, which could be using the melatonin signalling to synchronize their reproduction rhythms to the lunar cycle. © 2010 Elsevier B.V.

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