Institute of Applied Medicines and Research

Utter Pradesh, India

Institute of Applied Medicines and Research

Utter Pradesh, India
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Singh B.,Institute of Applied Medicines and Research | Singh R.,Institute of Applied Medicines and Research
Milchwissenschaft | Year: 2011

With the recent restriction of antibiotic use in food-producing animals the desire to develop effective human and animal-compatible, antibiotic alternatives have arisen. Recently, in vitro, natural proteins upon degradation by digestive enzymes or microbial enzymes have been shown to release antimicrobial peptides (AMPs), which exhibit unique mechanism to kill bacteria. Five strains of Lactobacillus helveticus were screened for antimicrobial activity exerted by the bovine skim milk whey supernatant. The cell-free extract of different strains of L. helveticus showed inhibition for both Gram positive and -negative pathogens. The agar well assay inhibition zone for Bacillus cereus, Streptococcus agalactiae and Staphylococcus aureus was 3.2, 3.0 and 2.4 cm, respectively. It showed more than 2.2 cm inhibition zones against Escherichia coli, Enterobacter faecium, Pseudomonas tragi, Serratia marcescens and Salmonella typhi. To achieve maximum antimicrobial activity, conditions were optimized. Purification of the desired peptide was carried out using gel filtration, ion exchange chromatography and HPLC In addition, these peptides also exhibited anti-oxidative and ACE inhibitory activity. These peptides may be beneficial for treatment against microbial infection and hypertension.

Ahmad V.,Institute of Applied Medicines and Research | Kumar L.,Institute of Applied Medicines and Research | Sajid Jamal Q.M.,Institute of Applied Medicines and Research | Dhama G.,Institute of Applied Medicines and Research | And 2 more authors.
International Journal of Pharmacy and Technology | Year: 2011

Plants are the oldest source of pharmacologically active compounds, and have provided humankind with many medically useful compounds for centuries. In this study a comparative evaluation of methanolic extract of leaves, stem of Eclipta alba and natural oils namely Clove oil, Olive oil, peppermint oil and eucalyptus were tested for antimicrobial activity by disc diffusion method against E. coli, E. cloacae, E. faecalis, P. vulgaris, S. aureus, K. pneumoniae, S. saprophyticus. It was observed that methnolic extract of leaf, stem and natural oils shown reliable Zone of inhibition (ZOI) against all tested microbes. The variable inhibition effect were observed with methanolic extract and natural oil with organisms to organism. Clove oil found most potent inhibitor among all tested agents. Maximum inhibitory effect of methanolic extract of leaves was found against E. cloacae and lowest against E. coli and S. aureus and methanolic extract of stem shown highest against S. saprophyticus and lowest against E. coli. The highest ZOI of clove oil was found against K. pneumoniae and lowest against E. coli Olive oil was found to be resistant against all microbes. Thus natural oils were found effective as positive control and were more effective as compared to methanolic extract of Eclipta alba.

Ojha S.K.,KIIT University | Mishra S.,KIIT University | Kumar S.,KIIT University | Mohanty S.S.,CG Brewery Nepal Pvt. Ltd. | And 3 more authors.
Journal of Environmental Biology | Year: 2015

With an objective to assess environmental management criteria of a vinasse treatment plant (VTP) and to evaluate the critical environmental parameters, a study was undertaken in a multi-product (packaged apple juice, distillery, brewery, packaged drinking water) brewery-cum-distillery unit. The facility with a volumetric loading rate of 11-15 kg COD, 3.6-4.5 h hydraulic retention time and 20 g l-1 VSS had a scientifically managed technically sound effluent treatment system. While the water quality parameters were found within the acceptable limits, there was 99.07% reduction in BOD5 from 43140.0 to 398.0 mg l- and 98.61% reduction in COD from 98003.0 to 1357.0 mg l-1. There was appreciable improvement in mixed liquor suspended solids (MLSS), mixed liquor volatile suspended solids (MLVSS) and sludge volume index (SVI). A striking feature was the integrated aerobic-anaerobic highly efficient Up-flow Anaerobic Sludge Blanket (UASB) treatment for biodegradation and energy production that reduced energy and space needs, producing utilisable end-products and net savings on the operational cost. The end-point waste management included terminal products such as fertile sludge, cattle feed supplement, recyclable water and biogas. Vast lagoons with combined aerobic-anaerobic approaches, biogasification unit, sludge recovery, remediated irrigable waterwere the notable attributes. © 2015 Triveni Enterprise, Lucknow (India).

Singh B.,Institute of Applied Medicines and Research | Chand R.,National Dairy Research Institute | Singh R.,Institute of Applied Medicines and Research
Milchwissenschaft | Year: 2011

Hypertension is the major risk factor for cardiovascular diseases, such as coronary heart disease, congestive heart failure and stroke. By lowering high blood pressure with antihypertensive treatment; the incidence and severity of these complications can be decreased. Several milk casein-derived peptides are able to lower blood pressure. Present research has been carried out to find out the effect of Lactobacillus helveticus NCDC 288 fermented bovine milk caseinderived peptides to inhibit Angiotensin-1 converting enzyme (ACE) activity. Bovine milk was fermented with Lb. helveticus NCDC 288. After incubation the curd was mixed properly and centrifuged. The peptides produced were separated with the help of Sephadex G-25 and HPLC using C-18 column. These peptides were tested in vitro for Angiotensin-1 converting enzyme (ACE) inhibitory activity and were found effective. The ACE inhibitory activity of HPLC fractions was assayed by the spectrometric method. The proposed method for ACE inhibitory activity here is based on color development by specific binding of 2,4,6-trinitro benzene sulfonate (TNBS) to the primary amine, and the fraction obtained from the 3 rd and 4 th peak of HPLC showed ACE inhibition 58 and 75%, respectively.

Kumar A.,Institute of Applied Medicines and Research | Tantry B.A.,Al Jouf University | Rahiman S.,Al Jouf University | Gupta U.,Institute of Applied Medicines and Research
Journal of Traditional Chinese Medicine | Year: 2011

Objective: To investigate antimicrobial potential of aqueous (infusions, decoctions) and methanlic extracts (1:2 and 1:5 concentrations) of Emblica officinalis (amla) against seven pathogenic bacteria namely Staphylococcs aureus, Staphylococcus saprophyticus, Escherichia coli, Enterococcus faecalis, Enterococcus cloacae, Proteus vulgaris and Klebsiella pneumoniae. Methods: The well diffusion technique was employed. The minimum inhibitory concentration (MIC) was determined using micro-broth dilution methods and phytochemical screening was done as per standard procedures. Results: Aqueous infusion extract of amla exhibited potent antimicrobial activity against E. cloacae followed by E. coli. Preliminary phytochemical analysis of E. officinalis aqueous extracts (infusions and decoctions) only showed presence of tannins, saponins, flavanoids, Terpenoids and phenols. MIC of aqueous extract of E. officinalis was most active against K. pneumoniae. Whereas MIC of methanol extract of E. officinalis shows maximum activity against E. coli. Conclusion: Emblica officinalis definitely possesses potent antimicrobial activities and this can serve as an important platform for the development of inexpensive, safe and effective medicines.

Sharma A.K.,P.A. College | Sharma A.K.,I F B Molecular Research Laboratory | Vats P.,Institute of Applied Medicines and Research | Malik A.,I F B Molecular Research Laboratory
Biochemical and Cellular Archives | Year: 2011

The antibacterial effect of silver nitrate has been evaluated to determine its effect on the biochemical profile and bacterial growth. The analysis of impact of silver salt on the bacterial growth curve showed that silver is more effective against E.coli than on S. typhi at a concentration of 0.001M while in case of S. typhi the growth was inhibited by the silver even at the minimum concentration of 0.0001M. Silver also showed its antibacterial effect by inhibiting the amino acid synthesis of bacterial cells. The MIC of silver nitrate was confirmed 0.001M against E. coli while for S. typhi the MIC was found 0.0001M. These results confirmed that silver is a potent antibacterial and can be used as an antibacterial agent for the treatment of bacterial infections.

Kumar A.,Institute of Applied Medicines and Research | Kumar S.,Institute of Applied Medicines and Research | Kumar V.,Institute of Applied Medicines and Research
Journal of Pure and Applied Microbiology | Year: 2011

The aim of this study was to isolate and identify the arsenic resistant bacteria. The experiment was conducted through January 2010 to May 2010 at Microbiology Laboratory of Institute of Applied Medicines and Research, Ghaziabad, Uttar Pradesh. The water samples were collected from different polluted water bodies. Twenty one arsenic resistant bacterial strains were isolated form polluted water. Arsenic resistant bacteria were isolated by growing on nutrient agar medium impregnated with high concentration (20 mg/mL) of As2O3 and As2S3 Four isolates were found more resistant and finally selected for their morphological and biochemical studies in details. All four isolates were able to tolerate very high concentration (> 0.2% or 20 mg/mL). The optimum temperature and pH for growth of all four strains were 30-37 °C and 7.0-8.0 respectively.

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