Institute of Applied Biotechnology

Taizhou, China

Institute of Applied Biotechnology

Taizhou, China
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Zhang X.,University of Shanghai for Science and Technology | Li H.,University of Shanghai for Science and Technology | Hou F.,University of Shanghai for Science and Technology | Yang Y.,University of Shanghai for Science and Technology | And 4 more authors.
Applied Surface Science | Year: 2017

In this work, metal-organic frameworks (MOFs) Mn-MIL-100 were first prepared, which were next used as templates to obtain the irregular porous Mn2O3 cubes through calcination with air at different temperature. The catalysts were characterized by N2 adsorption-desorption, X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscopy (TEM), H2-temperature program reduction (H2-TPR) and X-ray photoelectron spectroscopic (XPS). The catalytic activity for CO oxidation over Mn2O3 catalysts was investigated. It was found that calcination temperature had a strong effect on the structure and catalytic activity of Mn2O3 catalyst. Mn2O3 catalyst obtained by calcined at 700 °C (Mn2O3-700) showed a smaller specific surface area, but displayed a high catalytic activity and excellent stability with a complete CO conversion temperature (T98) of 240 °C, which was attributed to the unique structure, a high quantity of surface active oxygen species, smaller particle size, oxygen vacancies and good low temperature reduction behavior. The effect of water vapor on catalytic activity was also examined. The introduction of water vapor to the feedstock induced a positive effect on CO oxidation over Mn2O3-700 catalyst. Furthermore, no obvious drop is observed in activity over catalysts even in the presence of water vapor during 48 h. © 2017 Elsevier B.V.


Schoellhorn M.,Boehringer Ingelheim | Fischer S.,STAGE-Co | Wagner A.,Institute of Applied Biotechnology | Handrick R.,Institute of Applied Biotechnology | Otte K.,Institute of Applied Biotechnology
Biotechnology Progress | Year: 2017

In recent years, the number of complex but clinically effective biologicals such as multi-specific antibody formats and fusion proteins has increased dramatically. However, compared to classical monoclonal antibodies (mAbs), these rather artificially designed therapeutic proteins have never undergone millions of years of evolution and thus often turn out to be difficult-to-express using mammalian expression systems such as Chinese hamster ovary (CHO) cells. To provide access to these sophisticated but effective drugs, host cell engineering of CHO production cell lines represents a promising approach to overcome low production yields. MicroRNAs (miRNAs) have recently gained much attention as next-generation cell engineering tools. However, only very little is known about the capability of miRNAs to specifically increase production of difficult-to-express proteins. In a previous study we identified miR-143 amongst others to improve protein production in CHO cells. Thus, the aim of the present study was to examine if miR-143 might be suitable to improve production of low yield protein candidates. Both transient and stable overexpression of miR-143 significantly improved protein production without negatively affecting cell growth and viability of different recombinant CHO cells. In addition, mitogen-activated protein kinase 7 (MAPK7) was identified as a putative target gene of miR-143-3p in CHO cells. Finally, siRNA-mediated knock-down of MAPK7 could be demonstrated to phenocopy pro-productive effects of miR-143. In summary, our data suggest that miR-143 might represent a novel genetic element to enhance production of difficult-to-express proteins in CHO cells which may be partly mediated by down-regulation of MAPK7. © 2017 American Institute of Chemical Engineers.


Chen Z.,Taizhou University | Yang B.,Taizhou University | Hao Z.,Institute of Applied Biotechnology | Zhu J.,Taizhou University | And 2 more authors.
Journal of Plant Growth Regulation | Year: 2017

Lead (Pb) is a widespread ecosystem pollutant and affects food security and public health. Hydrogen sulfide (H2S) plays prominent roles in mediating a variety of responses to stresses. The effects of sodium hydrosulfide (NaHS), a fast releaser of H2S, on cauliflower (Brassica oleracea L. var botrytis L. cv. Xiahua 60 d) seed germination and seedling growth under lead acetate stress were investigated in the present study. Pb (0.25 and 0.5 mM) stresses markedly inhibited seed germination and seedling growth, whereas the inhibition was effectively mitigated by NaHS application. Germination percentage, root length, shoot length, and fresh weight of single seedling significantly increased. In addition, NaHS elevated endogenous H2S contents and reduced malonyldialdehyde, superoxide anion ((Formula presented.)), and hydrogen peroxide (H2O2) production, thereby preventing oxidative damage from Pb or Pb and antioxidant enzyme inhibitor (diethyldithiocarbamate or 3-amino-1,2,4-triazole) dual stresses. The protective roles of NaHS were equivalent to the ROS scavengers, 4,5-dihydroxy-1,3-benzene disulfonic acid? and N,N′-Dimethylthiourea. Moreover, NaHS elevated non-protein thiols and total glutathione levels to chelate Pb or scavenge ROS directly. Our results demonstrated the strong protective and antioxidant roles of H2S. © 2017 Springer Science+Business Media New York


Zhang X.,University of Shanghai for Science and Technology | Hou F.,University of Shanghai for Science and Technology | Yang Y.,University of Shanghai for Science and Technology | Wang Y.,Institute of Applied Biotechnology | And 2 more authors.
Applied Surface Science | Year: 2017

The paper presents a novel and facile method for preparing cauliflowerlike CeO2 through direct decomposition of cerium based metal-organic framework (MOF) Ce-BTC (BTC = 1,3,5-benzenetricarboxylic acid) straw in air. Several analytical tools such as Scanning electron microscopy (SEM), X-ray diffraction (XRD), Thermogravimetric (TG), N2 adsorption-desorption, Temperature programmed reduction (TPR), Raman, X-ray photoelectron spectroscopic (XPS) and Photoluminescence (PL) have been used to characterize Ce-BTC and CeO2. The Ce-BTC calcined at 500 °C (CeO2-500) maintains the morphology of its template “Ce-BTC” and forms a special cauliflower-like structure. XRD patterns showed that the catalyst has a perfect CeO2 crystal structure and has a smaller particle size. The prepared CeO2 cauliflowers exhibit excellent catalytic activities, long-term stability, and cycling stability for CO oxidation. The improved catalytic activities could be attributed to porous nanorods of CeO2 cauliflowers, which provide more active sites and oxygen vacancy for CO oxidation. © 2017 Elsevier B.V.


Zhang Y.,Jiangnan University | Luan H.,Institute of Agricultural Science in Jiangsu Coastal Areas | Wei Z.,Jiangnan University | Hao Z.,Institute of Applied Biotechnology | And 2 more authors.
Annals of Microbiology | Year: 2016

In the coming decades, exploitation of plant growth promoting bacteria (PGPB) will be a major trend in sustainable agriculture. Except for rhizobacteria, the bacteria inhabiting the non-rhizosphere could be considered the supplementary microbial source to exploit PGPB. Rare earth tailings soil (RETS) has caused a serious threat to sustainable agriculture due to excessive aluminum (Al) toxicity under acidic condition (pH < 5.5). The efficient restoration strategy is to combine phytoremediation and PGPB. The aim of this work was to screen honey-associated Bacillus strains as PGPB to improve barley (Hordeum vulgare L.) growth in RETS. Nine Bacillus strains from honey were initially selected with higher tolerance to acid (pH 4.0) and Al stress (1.5 mM). Only six strains among these nine strains could produce ACC deaminase. The nine strains were all efficient in dissolving phosphate, producing organic acid, siderophore, and IAA. The nine strains could promote barley seedlings growth mainly via production of organic acid, ACC deaminase. However, this growth promotion was weakened slightly by siderophore and phosphate solubilization under acidic condition. On the whole, inoculation with the nine strains obviously enhanced barley seedlings growth in RETS. Thus, the nine honey-associated Bacillus strains could be exploited as PGPB to enhance barley growth in RETS. © 2015, Springer-Verlag Berlin Heidelberg and the University of Milan.


Papavasileiou A.,Aristotle University of Thessaloniki | Madesis P.B.,Institute of Applied Biotechnology | Karaoglanidis G.S.,Aristotle University of Thessaloniki
Phytopathology | Year: 2016

Brown rot is a devastating disease of stone fruit caused by Monilinia spp. Among these species, Monilinia fructicola is a quarantine pathogen in Europe but has recently been detected in several European countries. Identification of brown rot agents relies on morphological differences or use of molecular methods requiring fungal isolation. The current study was initiated to develop and validate a high-resolution melting (HRM) method for the identification of the Monilinia spp. and for the detection of M. fructicola among other brown rot pathogens. Based on the sequence of the cytb intron from M. laxa, M. fructicola, M. fructigena, M. mumecola, M. linhartiana, and M. yunnanensis isolates originating from several countries, a pair of universal primers for species identification and a pair of primers specific to M. fructicola were designed. The specificity of the primers was verified to ensure against cross-reaction with other fungal species. The melting curve analysis using the universal primers generated six different HRM curve profiles, each one specific for each species. Ôhe HRM analysis primers specific to M. fructicola amplified a 120-bp region with a distinct melt profile corresponding to the presence of M. fructicola, regardless of the presence of other species. HRM analysis can be a useful tool for rapid identification and differentiation of the six Monilinia spp. using a single primer pair. This novel assay has the potential for simultaneous identification and differentiation of the closely related Monilinia spp. as well as for the differentiation of M. fructicola from other common pathogens or saprophytes that may occur on the diseased fruit. © 2016 The American Phytopathological Society.


Zhang Y.,Jiangnan University | Li X.,Jiangnan University | Li X.,Nanchang University | Hao Z.,Institute of Applied Biotechnology | And 3 more authors.
PLoS ONE | Year: 2016

For the more efficient detoxification of phenolic compounds, a promising avenue would be to develop a multi-enzyme biocatalyst comprising peroxidase, laccase and other oxidases. However, the development of this multi-enzyme biocatalyst is limited by the vulnerability of fungal laccases and peroxidases to hydrogen peroxide (H2 O2 )-induced inactivation. Therefore, H2 O2 -resistant peroxidase and laccase should be exploited. In this study, H2 O2 -stable CotA and YjqC were isolated from the outer coat of Bacillus altitudinis SYBC hb4 spores. In addition to the thermal and alkali stability of catalytic activity, CotA also exhibited a much higher H2 O2 tolerance than fungal laccases from Trametes versicolor and Trametes trogii. YjqC is a sporulation-related manganese (Mn) catalase with striking peroxidase activity for sinapic acid (SA) and sinapine (SNP). In contrast to the typical heme-containing peroxidases, the peroxidase activity of YjqC was also highly resistant to inhibition by H2 O2 and heat. CotA could also catalyze the oxidation of SA and SNP. CotA had a much higher affinity for SA than B. subtilis CotA. CotA and YjqC rendered from B. altitudinis spores had promising laccase and peroxidase activities for SA and SNP. Specifically, the B. altitudinis spores could be regarded as a multi-enzyme biocatalyst composed of CotA and YjqC. The B. altitudinis spores were efficient for catalyzing the degradation of SA and SNP in rapeseed meal. Moreover, efficiency of the spore-catalyzed degradation of SA and SNP was greatly improved by the presence of 15 mM H2 O2 . This effect was largely attributed to synergistic biocatalysis of the H2 O2 -resistant CotA and YjqC toward SA and SNP. © 2016 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


PubMed | Institute of Applied Biotechnology, Huanghuai University, University of Georgia and Jiangnan University
Type: Journal Article | Journal: International journal of molecular sciences | Year: 2016

Two CHI genes from Chitinolyticbacter meiyuanensis SYBC-H1 encoding chitinases were identified and their protein 3D structures were predicted. According to the amino acid sequence alignment, CHI1 gene encoding 166 aa had a structural domain similar to the GH18 type II chitinase, and CHI2 gene encoding 383 aa had the same catalytic domain as the glycoside hydrolase family 19 chitinase. In this study, CHI2 chitinase were expressed in Escherichia coli BL21 cells, and this protein was purified by ammonium sulfate precipitation, DEAE-cellulose, and Sephadex G-100 chromatography. Optimal activity of CHI2 chitinase occurred at a temperature of 40 C and a pH of 6.5. The presence of metal ions Fe(3+), Fe(2+), and Zn(2+) inhibited CHI2 chitinase activity, while Na and K promoted its activity. Furthermore, the presence of EGTA, EDTA, and -mercaptoethanol significantly increased the stability of CHI2 chitinase. The CHI2 chitinase was active with p-NP-GlcNAc, with the Km and Vm values of 23.0 mol/L and 9.1 mM/min at a temperature of 37 C, respectively. Additionally, the CHI2 chitinase was characterized as an N-acetyl glucosaminidase based on the hydrolysate from chitin. Overall, our results demonstrated CHI2 chitinase with remarkable biochemical properties is suitable for bioconversion of chitin waste.


Zhang X.,University of Shanghai for Science and Technology | Wang Y.,Institute of Applied Biotechnology | Hou F.,University of Shanghai for Science and Technology | Li H.,University of Shanghai for Science and Technology | And 3 more authors.
Applied Surface Science | Year: 2016

Flower-like Ag/ZnO samples were successfully fabricated via a simple and cost efficient method without surfactants. The morphologies, structural and optical properties of Ag/ZnO samples with various Ag content were investigated. The samples were systematically characterized by X-ray powder diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), N2 adsorption-desorption isotherm, diffuse reflectance spectroscopy (DRS), X-ray photoelectron spectroscopy (XPS), and photoluminescence spectroscopy (PL). It was found that ZnO was wurtzite phase and metallic Ag particles were wrapped by ZnO nanosheets. Compared with pure metallic Ag, the binding energy of Ag 3d for the Ag/ZnO samples distinctly shifted to the lower binding energy, which was attributed to the interaction between ZnO and Ag. With the increase of Ag content, surface plasmon absorption band of Ag/ZnO samples was obviously widened; meanwhile, PL intensity was decreased. The photocatalytic performance of Ag/ZnO samples were carried out by the degradation of methylene blue (MB) solution under visible light irradiation. The deposition of a certain amount of Ag was beneficial to the improvement of photocatalytic activity. The degradation rate of the Ag/ZnO sample with Ag/Zn ratio 1/20 was greater than fourfold times faster than that of ZnO. It was suggested that photoexcited electrons transferred from Ag to ZnO due to surface plasmon resonance (SPR), which could effectively reduce the recombination of electron-hole pairs and prolong lifetime of the electron-holes pairs, promoting the degradation efficiency. The deposition of a large amount of Ag was unfavorable for the formation of flower-like Ag/ZnO samples, and caused the decrease of specific surface area and the aggregation of Ag nanoparticles, leading to the reduction of photocatalytic performance. © 2016 Elsevier B.V.


Yang Y.,University of Shanghai for Science and Technology | Li H.,University of Shanghai for Science and Technology | Hou F.,University of Shanghai for Science and Technology | Hu J.,University of Shanghai for Science and Technology | And 2 more authors.
Materials Letters | Year: 2016

Flower-like ZnO/Ag nanocomposites were successfully synthesized via a simple, green and cost efficient method. In this method, the flower-like precursor was first prepared and subsequently calcined at 500 °C at 2 h. The XRD results confirmed the presence of pure wurtzite ZnO structure and metal Ag in flower-like precursor. The SEM and TEM results revealed that the obtained nanocomposites well inherited the morphology of the precursor and Ag particles deposited on the inside of the nanocomposites. The UV-vis diffuse reflection and PL spectra showed that ZnO/Ag nanocomposites exhibited tunable optical properties. Noteworthily, ZnO/Ag nanocomposites showed more efficient photocatalytic activity in the degradation of MB than that of pure ZnO under visible light irradiation. © 2016 Elsevier B.V.

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