Lin Y.Q.,Southwest University for Nationalities |
Wang G.S.,Southwest University for Nationalities |
Feng J.,Southwest University for Nationalities |
Huang J.Q.,Southwest University for Nationalities |
And 5 more authors.
Livestock Science | Year: 2011
The objective of the present study was to reveal the differences between yak (Bos grunniens) and cattle in energy metabolic characteristics and gene expressions in skeletal muscles. Activities of lactate dehydrogenase (LDH), malate dehydrogenase (MDH) and α-hydroxyacyl-CoA dehydrogenase (HOAD), which are involved in metabolism of carbohydrate and fatty acid respectively, were measured in longissimus dorsi and biceps femoris. Yak contained higher total LDH activity and higher proportion of LDH5 in longissimus dorsi than cattle (P<0.05), indicating its more anaerobic potential characteristics in carbohydrate metabolism. However, yak contained higher activities of MDH and HOAD (P<0.05) in biceps femoris than cattle, exhibiting high oxidative capacity under hypoxic environment. Semi-quantitative RT-PCR analysis of mRNA abundance showed that yak and cattle contained a similar level of adipocyte fatty acid-binding protein (A-FABP) in both longissimus dorsi and biceps femoris, while myosin heavy chain I (MyHC I) in longissimus dorsi and biceps femoris, and peroxisome proliferator-activated receptor coactivator 1α (PGC-1) level in biceps femoris were significant lower in yak compared with cattle (P<0.01 and P<0.05 respectively), indicating the anaerobic characteristic of yak skeletal muscles. In conclusion, our experiment showed that yak skeletal muscles exhibit different energy metabolic property and gene expressions compared to cattle. © 2010 Elsevier B.V.
Gao J.,Southwest University for Nationalities |
Jiang Z.-R.,Institute of Animal Science and Veterinary Medicine of Ganzi Prefecture |
Liu X.,Institute of Animal Science and Veterinary Medicine of Ganzi Prefecture |
Zhao Y.-H.,Institute of Animal Science and Veterinary Medicine of Ganzi Prefecture |
And 5 more authors.
Canadian Journal of Animal Science | Year: 2014
The objective of this study was to compare the MUC1 variable number tandem repeat polymorphisms with adjacent distribution regions in three yak (Bos grunniens) breeds/populations. A total of 215 yaks of three yak breeds/ populations (Maiwa yak breed, Jiulong yak breed and Changtai yak population) were surveyed by the polymorphisms of Mucin 1 gene (MUC1). Six MUC1 alleles (B, C, D, F, G and H) forming 16 genotypes were identified. Cloning and sequencing of these alleles demonstrated that they differed in the numbers of variable number tandem repeats (VNTR) units ranging from 14 to 20, and allele H (14 VNTR units) was a new allele in yaks and observed only in Maiwa yak with a very low frequency. Cluster analysis based on MUC1 polymorphisms suggested that Changtai yak population has a closer association with Maiwa yak breed than with the Jiulong yak breed, and may represent an independent yak breed/ population. These results provided useful genetic information on the three yak breeds/populations and could be used in yak breeding practice.
PubMed | Southwest University for Nationalities and Institute of Animal Science and Veterinary Medicine of Ganzi Prefecture
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015
The objective of this study was to compare 12 bp-duplication polymorphisms in exon 4 of the -casein gene among 3 breeds/populations of yak (Bos grunniens). Genomic DNA was extracted from yak blood or muscle samples (N = 211) and a partial sequence of exon 4 of -casein gene was amplified by polymerase chain reaction. A polyacrylamide gel electrophoresis assay of the products (169 bp) revealed 2 variants. These variants differed in a 12-bp duplication of the nucleotide sequence corresponding to amino acids 147-150 (Glu-Ala-Ser-Pro) or 148-151 (Ala-Ser-Pro-Glu). The genotype frequency and gene frequency of the 2 -casein variants differed among the 3 yak breeds/populations. The long form of the -casein gene was the predominant allele, and the Jiulong yak showed the highest frequency of the short form variant of the -casein gene. In addition, 2 nucleotide differences resulting in amino acid substitutions were also identified in yaks. These results are significant for designing a breeding strategy to improve the genetic makeup of yak herds.