Institute of Animal Reproduction and Food Research PAN

Olsztyn, Poland

Institute of Animal Reproduction and Food Research PAN

Olsztyn, Poland

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Frias J.,Institute Fermentaciones Industriales CSIC | Gulewicz P.,University of Technology and Life Sciences in Bydgoszcz | Martinez-Villaluenga C.,Institute Fermentaciones Industriales CSIC | Penas E.,Institute Fermentaciones Industriales CSIC | And 5 more authors.
Journal of Agricultural and Food Chemistry | Year: 2010

The selenium supply in almost all European countries is below the recommended daily intake, and different strategies are followed to fortify foods. In the present work, the influence of germination of garden cress (Lepidium sativum cv. Ogrodowa) in different selenium solutions (Na 2SeO3 and Na2SeO4) on Se uptake, total antioxidant capacity, glucosinolates, protein, and amino acids was studied. Cytotoxicity in HL-60 human leukemic cell line was also assessed. The addition of selenite (Na2SeO3) or selenate (Na 2SeO4) led to a significant increment in Se uptake in garden cress sprouts, and the highest Se content was observed at 8 mg/L in both inorganic Se solutions (36-38 ,μg/g of dm). The Se-enriched sprouts presented a large total antioxidant capacity (142-157 μmol of Trolox/g of dm), total glucosinolate content (99-124 μg/g of dm), protein (36-37% dm), and total essential amino acid content (40-41 g/100 g of protein), and no cytotoxicity on HL-60 human leukemic cells was observed. Garden cress sprouts obtained with selenite solution at 8 mg/L presented the best nutritional qualities and might provide a substantial proportion of Se in European diets. Bearing in mind the high nutritional value of sprouts, these may serve for the production of functional foods. ©2010 American Chemical Society.


Martinez-Villaluenga C.,Institute Fermentaciones Industriales CSIC | Penas E.,Institute Fermentaciones Industriales CSIC | Ciska E.,Institute of Animal Reproduction and Food Research PAN | Piskula M.K.,Institute of Animal Reproduction and Food Research PAN | And 3 more authors.
Food Chemistry | Year: 2010

Optimisation of the germination process of different cultivars of broccoli (Brassica oleracea var. italica cv. Lucky, cv. Tiburon and cv. Belstar) and radish (Raphanus sativus cv. Rebel and cv. Bolide) seeds in relation to the content of glucosinolates (GLS), vitamin C and total antioxidant capacity was carried out in order to maximise the health-promoting properties of Brassica sprouts. The content of total and individual GLS varied between species, among cultivars, and germination time. Glucoraphanin in broccoli and glucoraphenin in radish were the predominant GLS in raw seeds (61-77 and 63-129 μmol/g DM, respectively) and, although their content decreased during germination, they were maintained in rather large proportions in sprouts. Vitamin C was not detected in raw seeds and its content increased sharply in broccoli and radish sprouts (162-350 and 84-113 mg/100 g DM, respectively). Raw brassica seeds are an excellent source of antioxidant capacity (64-90 and 103-162 μmol Trolox/g DM in broccoli and radish, respectively) and germination led to a sharp increase. Germination of broccoli cv. Belstar and radish cv. Rebel for 4 days provided the largest glucoraphanin and glucoraphenin content, respectively, and also brought about large amounts of vitamin C and antioxidant capacity. © 2009 Elsevier Ltd. All rights reserved.

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