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Hosamani M.,Indian Veterinary Research Institute | Basagoudanavar S.H.,Indian Veterinary Research Institute | Tamil Selvan R.P.,Indian Veterinary Research Institute | Das V.,Indian Veterinary Research Institute | And 4 more authors.
Archives of Virology | Year: 2015

Reliable diagnostic tests that are able to distinguish infected from vaccinated animals are a critical component of regional control programs for foot-and-mouth disease (FMD) in the affected countries. Non-structural protein (NSP) serology based on the 3ABC protein has been widely used for this purpose, and several kits are commercially available worldwide. This report presents the development of a 3ABC-antigen-based indirect ELISA, employing a peroxidase-conjugated protein G secondary antibody that can detect antibodies from multiple species. Recombinant 3ABC protein was expressed in insect cells and purified using affinity column chromatography. Using this protein, an indirect ELISA was developed and validated for the detection of NSP antibodies in serum samples collected from animals with different status of FMD. Diagnostic sensitivity and specificity were found to be 95.8 (95 % CI: 92.8–97.8) and 97.45 % (95 % CI: 94.8–99.0), respectively. The in-house ELISA compared well with the commercially available prioCHECK FMDV NS-FMD kit, with a high agreement between the tests, as determined by the kappa coefficient, which was 0.87. The in-house ELISA showed higher sensitivity for detecting vaccinated and subsequently infected animals, when compared to the reference test. Both of the tests were able to detect NSP antibodies as early as 7–8 days after experimental infection. © 2015, Springer-Verlag Wien.

Patil S.S.,Project Directorate on Animal Disease Monitoring and Surveillance | Hemadri D.,Project Directorate on Animal Disease Monitoring and Surveillance | Sreekala K.,Project Directorate on Animal Disease Monitoring and Surveillance | Veeresh H.,Project Directorate on Animal Disease Monitoring and Surveillance | And 3 more authors.
Indian Journal of Animal Sciences | Year: 2012

Two bovine herpesvirus-1 (BoHV-1) isolates previously recovered from 2 animals showing different clinical symptoms, i.e. respiratory disease and balanoposthitis, were partially nucleotide sequenced at the gB gene. Aligned nucleotide sequences revealed high degree of identity among all the alphaherpesviruses compared in the study. The nucleotide sequence identity among the Indian isolates varied from 99-99.5% and so was their identity with reference Cooper strain. The phylogenetic analysis indicated that both the isolates belonged to subtype 1.1 despite being isolated from animals with different clinical manifestations.

Dhanush Krishna B.,KVAFSU | Rao S.,KVAFSU | Satyanarayana M.L.,KVAFSU | Byregowda S.M.,KVAFSU | Byregowda S.M.,Institute of Animal Health and Veterinary Biologicals
Indian Journal of Animal Research | Year: 2015

The aim of the study was to compare the effects of Eugenia jambolana (EJ) extract and glibenclamide on streptozotocin (STZ) induced hepatotoxicity in rats. Wistar rats were divided into four groups: untreated control (UC), untreated diabetic (UD), EJ (200 mg/kg) treated diabetic (ED) and glibenclamide (0.6 mg/kg) treated diabetic (GD) orally for 45 days. There was various evidence of hepatotoxicity, including significant increase (P<0.001) in serum transaminases activity and histopathological findings in UD rats. In ED group, cellular injury was minimal and the hepatocytes maintained a better morphology when compared to UD rats. The substantially elevated serum transaminases were restored towards normal by the extracts. Findings indicate that EJ extract may have a preventive effect on STZ induced hepatotoxicity and also has potential hepatoprotective activity when compared to GD rats. © 2014, Indian Journal of Animal Research. All Rights Reserved.

Chandrakala G.K.,Institute of Animal Health and Veterinary Biologicals
Pakistan Journal of Pharmaceutical Sciences | Year: 2010

The leaves of Morus alba Linn. (Family: Moraceae) commonly known as mulberry are mainly used as food for the silkworms and they are sometimes eaten as vegetable or used as cattle fodder in different parts of the world. The effect of Morus alba on the immune system was evaluated by using different experimental models such as carbon clearance test, cyclophosphamide induced neutropenia, neutrophil adhesion test, effect on serum immunoglobulins, mice lethality test and indirect haemagglutination test. Methanolic extract of Morus alba was administered orally at low dose and high dose of 100 mg/kg and 1 g/kg respectively and Ocimum sanctum (100 mg/kg, po) was used as standard drug. Morus alba extract in both doses increased the levels of serum immunoglobulins and prevented the mortality induced by bovine Pasteurella multocida in mice. It also increased the circulating antibody titre in indirect haemagglunation test. On the other hand, it showed significant increase in the phagocytic index in carbon clearance assay, a significant protection against cyclophosphamide induced neutropenia and increased the adhesion of neutrophils in the neutrophil adhesion test. Hence, it was concluded that Morus alba increases both humoral immunity and cell mediated immunity.

Begam H.,Jadavpur University | Mandal S.,Jadavpur University | Chanda A.,Jadavpur University | Mukherjee J.,Institute of Animal Health and Veterinary Biologicals | Nandi S.K.,West Bengal University of Animal and Fishery Sciences
Transactions of the Indian Ceramic Society | Year: 2014

Addition of dopants in biomaterial has emerged as a vital regulator of bone formation and bone regeneration due to their significant role in the biological processes. We report the effect of zinc ion doping on various properties of biphasic calcium phosphate (BCP) dense body in vitro and in vivo. Zinc (<3 wt%) was successfully incorporated into both calcium hydroxyapatite (HAp) and β-tricalcium phosphate (TCP) via simple wet chemical route. BCP pellets were prepared by mixing HAp and TCP in a definite proportion, compacted and then sintered. The sintered pellets were checked with XRD, FTIR in addition to hardness and fracture toughness. A systematic in vivo study was conducted to evaluate healing of surgically created defects on the femur of rabbit after implantation of doped and undoped dense BCP for 4 months. Characterization of these sintered dense bodies revealed that zinc dopant had marked influence on the physical properties of the dense bodies as well as their mechanical properties. Fluorochrome labelling, histological analysis and radiology were performed post operation after two and four months to study angiogenesis, bone formation and osseous ingrowth. The findings suggested that addition of Zn dopants to the BCP ceramics demonstrated excellent bone formation and that may pave the new insight in bone tissue engineering. © 2014 The Indian Ceramic Society.

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