Institute of Animal Biology

L'viv, Ukraine

Institute of Animal Biology

L'viv, Ukraine
Time filter
Source Type

Klymyshin D.O.,Ivan Franko National University of Lviv | Klymyshin D.O.,Lviv National Medical University | Klymyshin D.O.,Institute of Animal Biology | Honchar M.A.,Institute of Animal Biology | And 2 more authors.
Microbiology (Russian Federation) | Year: 2014

Streptomyces echinatus Lv 22 (=DSM 40730) produces an anthracycline antibiotic aranciamycin. Development of DNA transfer using conjugation from Escherichia coli into this strain is described. Various replicative plasmids (pKC1139, pKC1218E, pSOK1O1, pCHZ101) as well as actinophage (φC31- and VWB-based vectors pSET152 and pVWB, respectively, were transferred from E. coli ET12567 (pUB307) into the S. echinatus at a frequency ranging from 2.4 × 10-3 to 1.6 × 10-4. The transconjugants did not differ from wild type in their ability to produce aranciamycin and morphological features. There is one attB site for pSET152 and pVWB integrative plasmids in the S. echinatus chromosome. Developed DNA transfer system was used for expression of heterologous regulatory genes in S. echinatus cells. Expression of relA gene of ppGpp synthetase increased antibiotic production in S. echinatus. The absA2 gene of S. ghanaensis appears to play a negative role in the control of aranciamycin biosynthesis. Additional copies of absA2 leads to inhibition of aranciamycin's production. absB and afsS had no effect on aranciamycin biosynthesis as well as on the morphological features of S. echinatus. Obtained results indicate efficiency of the developed system for gene cloning in S. echinatus. © 2014 Pleiades Publishing, Ltd.

Klymyshin D.O.,Lviv National Medical University | Klymyshin D.O.,Institute of Animal Biology | Klymyshin D.O.,Ivan Franko National University of Lviv | Stephanyshyn O.M.,Institute of Animal Biology | Fedorenko V.O.,Lviv National Medical University
Cytology and Genetics | Year: 2016

Stringent response is a pleiotropic physiological response of cells caused by deficiency of aminoacetylated tRNAs and, correspondingly, by the arrest of protein synthesis. This response can experimentally be induced by amino acid deficiency in a culture medium and limitation of the aminoacylation capacity of tRNA molecules even in the presence of the respective amino acids in the cell. Many traits of this response indicate its dependence on the accumulation of ppGpp molecules. There are links between the growth rate of actinomycetes and the biosynthesis of secondary metabolites by the bacteria. In particular, it has been established that the introduction of additional relA gene copies of the ppGpp synthetase can affect the production of antibiotics in streptomycetes. The survey presents the authors’ own experimental data obtained in the studies on the effect of heterologous relA gene expression in Streptomyces nogalater, the nogalamycin producer. © 2016, Allerton Press, Inc.

Syrvatka V.J.,Institute of Animal Biology | Slyvchuk Y.I.,Institute of Animal Biology | Rozgoni I.I.,Institute of Animal Biology | Gevkan I.I.,Institute of Animal Biology | Overchuk M.O.,Ukrainian Academy of Sciences
Progress in Biomedical Optics and Imaging - Proceedings of SPIE | Year: 2014

Modern routine enzyme immunoassays for detection and quantification of biomolecules have several disadvantages such as high cost, insufficient sensitivity, complexity and long-term execution. The surface plasmon resonance of silver nanoparticles gives reasons of creating new in the basis of simple, highly sensitive and low cost colorimetric assays that can be applied to the detection of small molecules, DNA, proteins and pollutants. The main aim of the study was the improving of enzyme immunoassay for detection and quantification of the target molecules using silver nanoparticles. For this purpose we developed method for synthesis of silver nanoparticles with hyaluronic acid and studied possibility of use these nanoparticles in direct determination of target molecules concentration (in particular proteins) and for improving of enzyme immunoassay. As model we used conventional enzyme immunoassays for determination of progesterone and estradiol concentration. We obtained the possibility to produce silver nanoparticles with hyaluronan homogeneous in size between 10 and 12 nm, soluble and stable in water during long term of storage using modified procedure of silver nanoparticles synthesis. New method allows to obtain silver nanoparticles with strong optical properties at the higher concentrations -60-90 μg/ml with the peak of absorbance at the wavelength 400 nm. Therefore surface plasmon resonance of silver nanoparticles with hyaluronan and ultraviolet-visible spectroscopy provide an opportunity for rapid determination of target molecules concentration (especial protein). We used silver nanoparticles as enzyme carriers and signal enhancers. Our preliminary data show that silver nanoparticles increased absorbance of samples that allows improving upper limit of determination of estradiol and progesterone concentration. © 2014 SPIE.

Simonova M.I.,Institute of Animal Biology | Boretska N.I.,Institute of Animal Biology | Kaminska M.V.,Institute of Animal Biology | Nechay H.I.,Institute of Animal Biology | And 2 more authors.
Ukrain'skyi Biokhimichnyi Zhurnal | Year: 2010

Supplementation of rats' diet with β-carotene or biomass of carotene producing yeast Phaffia rhodozyma caused a decrease of aminotransferases in the blood serum as well as a decrease of lipid peroxidation products and protein carbonil groups in the liver, brain and myocardium tissues of animals treated with tetrachloromethane. When compared to the control group the activity of superoxide dismutase, catalase and glutathione peroxidase in the liver of carotene fed rats were respectively 1.6, 2.2, and 1.5-fold higher. Thus, these supplements to standard diet slow down development of tetrachlorometane mediated oxidative stress in rats.

Loading Institute of Animal Biology collaborators
Loading Institute of Animal Biology collaborators