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He H.,Affiliated Hospital of Nantong UniversityNantong 226019Jiangsu China | Chen Y.-P.,Nantong Tongda Chemicals Safety Evaluation Center Conantong 226019Jiangsu China | Li X.-D.,Nantong Tongda Chemicals Safety Evaluation Center Conantong 226019Jiangsu China | Wang Y.-F.,Institute of Analytical Chemistry for Life Science | Hu Y.-L.,Nantong Tongda Chemicals Safety Evaluation Center Conantong 226019Jiangsu China
Journal of Biomedical Materials Research - Part B Applied Biomaterials | Year: 2016

Various types of wound dressings have been used to treat complex infections in diabetes mellitus. This study is the first to evaluate the healing effects using a two-stage dressing in infected diabetic wounds. A two-stage antibacterial hydrogel dressing (two-stage dressing) was established with two time phases, an antibacterial phase and a drug release phase. We established each phase by using a swelling and rate of drug release test. These results suggested that the antimicrobial phase is activated as soon as the two-stage dressing attaches to the skin. The drugs in the drug release layer of the dressing were released to a greater extent than expected 20-36 h after attachment to the skin, likely due to extensive water absorption. Histological analysis and measurement of vascular endothelial growth factor expression through in vivo testing suggested that the benefits of a two-stage dressing include rapid antibacterial properties, sustained drug release, and promotion of wound healing through cell proliferation as compared with the traditional composite antibacterial hydrogel dressing. Further in vivo tests confirmed that separation of the antibacterial and drug-releasing properties, along with biocompatibility and rapid wound closure rates made two-stage dressings suitable for healing of infected wounds. © 2015 Wiley Periodicals, Inc.

Yu C.,Institute of Analytical Chemistry for Life Science | Yu C.,Nantong University | Zhou X.,Nantong University | Gu H.,Nantong University
Electrochimica Acta | Year: 2010

This paper reports on the fabrication and characterization of hemoglobin (Hb)-colloidal silver nanoparticles (CSNs)-chitosan film on the glassy carbon electrode and its application on electrochemical biosensing. CSNs could greatly enhance the electron transfer reactivity of Hb as a bridge. In the phosphate buffer solution with pH value of 7.0, Hb showed a pair of well-defined redox peaks with the formal potential (E0′) of -0.325 V (vs. SCE). The immobilized Hb in the film maintained its biological activity, showing a surface-controlled process with the heterogeneous electron transfer rate constant (ks) of 1.83 s-1 and displayed the same features of a peroxidase in the electrocatalytic reduction of oxygen and hydrogen peroxide (H2O2). The linear range for the determination of H2O2 was from 0.75 μM to 0.216 mM with a detection limit of 0.5 μM (S/N = 3). Such a simple assemble method could offer a promising platform for further study on the direct electrochemistry of other redox proteins and the development of the third-generation electrochemical biosensors. © 2010 Elsevier Ltd All rights reserved.

Pan Z.-Q.,Institute of Analytical Chemistry for Life Science | Pan Z.-Q.,Nantong University | Shi C.-G.,Nantong University | Fan H.,Institute of Analytical Chemistry for Life Science | And 10 more authors.
Sensors and Actuators, B: Chemical | Year: 2012

This paper presents both electrochemical and electrochemiluminescence (ECL) detections based on the layer-by-layer (LBL) films fabricated with hemoglobin (Hb) and the optimal nanocomposite containing multiwall carbon nanotubes (MWCNTs) and CdS quantum dots (QDs). Hb and the MWCNT-CdS nanocomposite were electrostatically assembled on the chitosan (CS) modified glassy carbon electrode (GCE) forming with up to six bilayer films. Our results on cyclic voltammograms (CVs) revealed that the redox peak currents of Hb at the six-bilayer film ({MWCNT-CdS/Hb} 6) modified GCE were the largest. Hydrogen peroxide (H 2O 2) could be stably detected using the six bilayer films modified GCE with a wide linear range from 0.125 μM to 1.20 mM. Nitrite (NO 2 -) could also be determined through CdS QDs of the multilayer films based on stable ECL intensities with a linear range from 0.60 μM to 0.80 mM. © 2012 Elsevier B.V.

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