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Thessaloníki, Greece

Esteban R.,Institute of Agrobiotechnology | Barrutia O.,University of the Basque Country | Artetxe U.,University of the Basque Country | Fernandez-Marin B.,University of the Basque Country | And 2 more authors.
New Phytologist

Photosynthetic pigment composition has been a major study target in plant ecophysiology during the last three decades. Although more than 2000 papers have been published, a comprehensive evaluation of the responses of photosynthetic pigment composition to environmental conditions is not yet available. After an extensive survey, we compiled data from 525 papers including 809 species (subkingdom Viridiplantae) in which pigment composition was described. A meta-analysis was then conducted to assess the ranges of photosynthetic pigment content. Calculated frequency distributions of pigments were compared with those expected from the theoretical pigment composition. Responses to environmental factors were also analysed. The results revealed that lutein and xanthophyll cycle pigments (VAZ) were highly responsive to the environment, emphasizing the high phenotypic plasticity of VAZ, whereas neoxanthin was very stable. The present meta-analysis supports the existence of relatively narrow limits for pigment ratios and also supports the presence of a pool of free 'unbound' VAZ. Results from this study provide highly reliable ranges of photosynthetic pigment contents as a framework for future research on plant pigments. © 2014 New Phytologist Trust. Source

Papaefthimiou D.,Institute of Agrobiotechnology | Aligizaki K.,Aristotle University of Thessaloniki | Nikolaidis G.,Aristotle University of Thessaloniki
Harmful Algae

The clarification of the identity of the prominent Dinophysis morphotype in Greek coastal waters, characterized until now as D. cf. acuminata, has become a major challenge since the first DSP outbreak in the year 2000. So far, definite identification of the morphotype at the species level has been a taunting task, since both morphological and common molecular tools have proved fairly inefficient towards this direction. Recently, the mitochondrial gene region encoding for the cytochrome c oxidase subunit I (cox1) has been opted for its efficiency to discriminate at the species level within the Dinophysis acuminata complex. Using both ribosomal and mitochondrial genetic sequence data, we have shown that the species D. cf. acuminata described from Greek coastal waters is actually more related to D. ovum. The implications involved with present molecular tools in deciphering taxonomic relationships among species belonging to the genus Dinophysis are also discussed. © 2010 Elsevier B.V. Source

Bosmali I.,Technological Educational Institute of Larissa | Ganopoulos I.,Aristotle University of Thessaloniki | Ganopoulos I.,Institute of Agrobiotechnology | Madesis P.,Institute of Agrobiotechnology | And 2 more authors.
Food Research International

Lentil (Lens culinaris) is an important legume crop worldwide, consumed as dried seeds. Correct identification of lentil varieties is important in order to ensure food quality, safety, authenticity and health for consumers as well as high price from elite varieties for farmers and industry. Recently, DNA-based methods like the molecular markers microsatellites (SSRs) for nuclear DNA or the DNA barcoding which uses chloroplast or nuclear DNA have been developed for plant species or variety identification, for genotyping and for identification of their ingredients in the final food products. Here we have integrated High Resolution Melting (HRM) analysis, coupled with five SSR markers in parallel with rpoC1 chloroplast DNA barcode targeting region, in order to facilitate the identification of Protected Geographical Indication (PGI) lentil variety 'Eglouvi'. The five SSR loci used were informative and generated a unique melting curve profile of microsatellites for each of the ten varieties tested. SSRs enabled the distinction and identification of the "Eglouvi" lentil PGI variety and furthermore they allowed the traceability of "Eglouvi" and the identification of lentil varieties admixtures of 50%. In addition, the application of the Barcode DNA High Resolution Melting (Bar-HRM) method on the species specific plant DNA barcoding region rpoC1, allowed not only the identification of adulterations but also the quantification of the most common lentil admixture. Bar-HRM detected Vicia sativa adulterants in Lens esculentum pure seed mix as low as 1:100. Hence, these assays provided flexible, cost-effective, and closed-tube SSR-HRM and Bar-HRM genotyping methods, well suited to identify adulterants in variety and species level and to food forensic uses in food products. © 2011 Elsevier Ltd. Source

Madesis P.,Institute of Agrobiotechnology | Ganopoulos I.,Aristotle University of Thessaloniki | Anagnostis A.,Institute of Agrobiotechnology | Tsaftaris A.,Aristotle University of Thessaloniki | Tsaftaris A.,Institute of Agrobiotechnology
Food Control

The Leguminoseae family includes several economically important genera like legumes. Legumes are the second most important agricultural family after cereals in terms of harvested area and production. Legumes are key elements in the Mediterranean diet and as winter annuals with nitrogen fixing capacity, they are extremely important for sustainable agriculture worldwide. We report here the application of the Bar-HRM (Barcode DNA High Resolution Melting) analysis method with the universal plant DNA barcoding region trnL which allowed the identification, adulteration and quantification of major Greek and Mediterranean in general bean species. Bar-HRM detected Lupinus spp. adulterants in Glycine max flour as low as 1:100. Moreover, the method was coupled with the rapid Phire PCR kit that does not require prior DNA purification. This makes the method a very fast and effective tool for barcoding Legumes and particularly for the crops examined not only for their authenticity but for quantitative detection of purity of their seeds or their processed food and feed products. © 2011 Elsevier Ltd. Source

Papaefthimiou D.,Institute of Agrobiotechnology | Papaefthimiou D.,Aristotle University of Thessaloniki | Kapazoglou A.,Institute of Agrobiotechnology | Tsaftaris A.S.,Institute of Agrobiotechnology | Tsaftaris A.S.,Aristotle University of Thessaloniki
Physiologia Plantarum

A number of genes are involved in the vernalization pathway, such as VRN1, VRN2 and VRN3/FT1, whose function has been studied in barley and wheat. However, the function of the flowering and vernalization integrator SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) has not been well studied in Triticeae, and particularly in barley. Herein, we cloned and characterized two barley SOC1-like homologs, HvSOC1-like1 and HvSOC1-like2. Primary sequence analysis of the predicted HvSOC1-like1 and HvSOC1-like2 proteins showed that they are members of the type II MADS-box protein family. Phylogenetic analysis placed the predicted proteins with other SOC1 and SOC1-like proteins from different species neighboring those from other cereal plant species. Primary and secondary structures of the predicted proteins are conserved to each other and more distant to the recently identified barley ODDSOC1 proteins. Genomic organization of HvSOC1-like1 is very similar to the Arabidopsis and Brachypodium SOC1 genes and localized in highly syntenic chromosomal regions. Regulatory cis-acting elements detected in the HvSOC1-like1 promoter include the CArG-box, implicated in the regulation of SOC1 expression in Arabidopsis. Both HvSOC1-like1 and HvSOCI-like2 are expressed in vegetative and reproductive tissues and at different stages of seed development. Both are upregulated in a particular seed developmental stage suggesting their possible implication in seed development. Furthermore, HvSOC1-like1 was induced in two winter barley cultivars after vernalization treatment pointing to its probable involvement in the vernalization process. The study of the SOC1 genes reported here opens the way for a better understanding of both the vernalization process and seed development and germination in this important cereal crop. © 2012 Physiologia Plantarum. Source

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