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Lee B.H.,Kyung Hee University | Nam T.G.,Kyung Hee University | Park W.J.,Gangneung - Wonju National University | Kang H.,Kyung Hee University | And 4 more authors.
Food Science and Biotechnology | Year: 2015

Chrysanthemum indicum Linné, belonging to the family Compositae, is a perennial herb that is widespread in Korea. C. indicum Linné flowers have been traditionally used as an oriental medicine for treatment of infectious diseases. The chemical compositions, antioxidant capacity, and neuroprotective effects of volatile components in essential oils from C. indicum Linné flowers were investigated. GC-MS analysis of essential oils revealed the 15 major components of 1,8-cineole, o-cymene, camphor, pinocarvone, chrysanthenyl acetate, bornyl acetate, trans-caryophyllene, terpinen-4-ol, umbellulone, trans-pinocarveol, cis-verbenol, borneol, α-terpineol, caryophyllene oxide, and thymol. Thymol had the highest antioxidant capacity. Essential oils and thymol dose-dependently decreased intracellular oxidative stress, exhibited anticholinesterase activities, and increased the cell viability of neuronal PC-12 cells. Essential oils and thymol from C. indicum Linné flowers can be sources of natural antioxidants and functional foods. © 2015, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht. Source


Eum D.-Y.,Research Institute for Natural science | Byun J.-Y.,Research Institute for Natural science | Yoon C.-H.,Research Institute for Natural science | Seo W.-D.,NICS | And 7 more authors.
Anti-Cancer Drugs | Year: 2011

A combined treatment with conventional chemotherapies can enhance the effectiveness of chemotherapeutic agents against cancers. Here, we have shown that the naturally occurring triterpenoids synergistically enhance the response of cervical cancer cells to taxol. Of the triterpenoid compounds, pristimerin enhanced the anticancer effect of taxol with the highest efficiency by combination. Pristimerin synergizes with taxol to inhibit clonogenic survival and tumor growth in nude mice, and to enhance cell death in cervical cancer cells. A combined treatment with taxol and pristimerin induced cervical cancer cell death by increasing intracellular reactive oxygen species levels, upregulation of death receptor death receptor 5 (DR5), activation of Bax, and dissipation of mitochondrial membrane potential. Treatment with N-acetyl-L-cysteine, a thiol-containing antioxidant completely blocked combined treatment-induced Bax translocation as well as DR5 upregulation. Moreover, inhibition of Jun N-terminal kinase/c-Jun pathway attenuated cell death by blocking DR5 upregulation and Bax activation. These results indicate that the triterpenoid, pristimerin, synergistically enhances taxol response of cervical cancer cells through DR5 expression and Bax activation. Furthermore, the reactive oxygen species-dependent activation of the Jun N-terminal kinase/c-Jun pathway is required for the DR5 upregulation and Bax activation. The molecular mechanism revealed by this study may aid in the design of future combination cancer therapies against cells with intrinsically reduced sensitivity to taxol. © 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins. Source


Dey S.R.,Gyeongsang National University | Deb G.K.,Gyeongsang National University | Ha A.N.,Gyeongsang National University | Lee J.I.,Gyeongsang National University | And 4 more authors.
Theriogenology | Year: 2012

The present study examined the effect of coculturing cumulus oocyte complexes (COCs) and denuded oocytes (DOs) during in vitro maturation (IVM) on nuclear and cytoplasmic maturation, zona pellucida (ZP) hardening, the pattern of fertilization and glutathione peroxidase 1 (GPX1) gene expression in the oocyte. Furthermore, the rate of embryonic development and the quality of blastocysts were examined for both COCs and DOs. Three IVM conditions were studied: 1) the coculture of 12 COCs and 60 DOs, 2) COC control with 12 COCs, and 3) DO control with 60 DOs. The IVM was performed in a 120-μl droplet of TCM199-based IVM medium. Following IVM, in vitro fertilization (IVF) and in vitro culture (IVC) were conducted separately for the COCs and DOs (DO coculture) from the IVM coculture group. Coculturing COCs and DOs increased the percentage of oocytes reaching the blastocyst stage and the total number of cells per blastocyst in both the COC coculture (44.4 ± 8.6 vs 26.7 ± 9.7%, P < 0.01, and 137.9 ± 24.9 vs 121.7 ± 21.1, P < 0.05) and the DO coculture (20.5 ± 5.0 vs 11.1 ± 2.5%, P < 0.01, and 121.9 ± 27.5 vs 112.3 ± 33.2, P < 0.05) compared to their respective control groups. The synergistic effects of coculturing were detected as increased nuclear and cytoplasmic maturation, the prevention of ZP hardening, increased monospermic fertilization and increased expression of GPX1 in the oocytes in response to endogenous oocyte-secreted factors. In conclusion, coculturing COCs and DOs may be an effective culture system for both intact COCs and immature DOs. © 2012 Elsevier Inc. Source


Fakruzzaman M.,Gyeongsang National University | Bang J.-I.,Gyeongsang National University | Lee K.-L.,Gyeongsang National University | Kim S.-S.,Gyeongsang National University | And 8 more authors.
Animal Reproduction Science | Year: 2013

The aim of this study was to investigate the developmental rate, lipid and mitochondrial distribution and gene expression in oocyte-derived embryos selected on the basis of brilliant cresyl blue (BCB) staining. Lipid content and mitochondrial distribution in Day 8 blastocysts were evaluated by fluorescence intensity, while gene expression was analyzed by real-time PCR. The proportion of blastocysts (30.9%) was greater (P< 0.05) in BCB+ than in BCB- oocytes (13%) but not different (P> 0.05) from the control group (28.2%). Total cell number was also greater in BCB+ (155.1 ± 36.2) than in BCB- (116.6 ± 40.5) and control (127.5 ± 35.7) blastocysts. Furthermore, the apoptotic cell number was less in BCB+ (3.7 ± 4.4) than in BCB- blastocysts (8.7 ± 8.7) but not different from the control group (5.9 ± 3.9). BCB+ embryos contained more mitochondria compared to BCB- embryos (P< 0.05). There was no significant difference in intercellular lipid accumulation in embryos from all groups. Interferon-τ (IFNτ), transforming growth factor β1 (TGFB1) and secreted seminal-vesicle Ly-6 protein 1 (SSLP1) gene expression was greater in BCB+ than in BCB- blastocysts. By contrast, Bcl2-associated X protein (BAX) and heterogeneous nuclear ribonucleoprotein A2/B1 (HNRNPA2B1) gene expression was greater in BCB- than in BCB+ and control embryos. In conclusion, oocyte-derived embryos selected on the basis of BCB staining showed differences in developmental rate, quality, mitochondrial content and target gene expression compared to control embryos. © 2013 Elsevier B.V. Source


Cho S.-J.,Gyeongsang National University | Lee Y.S.,Gyeongsang National University | Lee J.-I.,Gyeongsang National University | Bang J.-I.,Gyeongsang National University | And 4 more authors.
Cellular Reprogramming | Year: 2010

The production of transgenic animals is highly desirable for biotechnology and basic research. We investigated the reproductive ability of a red fluorescence protein (RFP) transgenic cloned male cat (RFP TG cat) by natural mating with a domestic female cat. The RFP expression levels were examined in early embryogenesis, tissues from 45-day-old two fetuses, and four RFP TG cat offspring. The RFP gene was detected in tissue samples from one dead kitten, including several organs and the skin. Also, under a fluorescent light source, we were able to directly detect the RFP expression of in in vitro-produced blastocysts derived with sperm from the RFP TG cat. These results indicate that the RFP TG cat exhibits normal reproductive fertility, stable germ-line transmission of the RFP transgene, and characteristic RFP expression in its offspring. We isolated feline neural progenitor cells from a 45-day-old fetus derived from the natural mating of the RFP TG cat with a domestic female cat. Isolated brain and retinal progenitor cells were successfully passaged at least four times postisolation (day 23), and showed a high RFP expression level. This method of producing genetically modified cloned cats will be important for generating biomedical models of human diseases. © 2010 Mary Ann Liebert, Inc. Source

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